三疣梭子蟹(Portunus trituberculatus)胞内氯离子通道蛋白基因克隆及其表达分析     

王渝  吕建建  刘萍  高保全  李健  陈萍 《海洋与湖沼》2014,45(6):1359-1366

本研究克隆了三疣梭子蟹(Portunus trituberculatus)胞内氯离子通道蛋白基因,命名为Pt CLIC。该基因c DNA全长2000bp,5’和3’非编码区(UTR)长分别为76bp和1162bp,开放阅读框长762bp,推测编码253个氨基酸,预测分子量为29.2k Da,理论等电点为5.93。生物信息学分析表明,Pt CLIC基因中未发现跨膜结构域,属于不稳定蛋白;同源性分析表明,Pt CLIC基因编码的氨基酸序列与蚤状溞(Daphnia pulex)CLIC基因的同源性高达83%;系统进化分析表明,三疣梭子蟹与拟穴青蟹首先聚为一支;实时荧光定量RT-PCR分析表明,Pt CLIC基因在选取的所有组织中均有表达,在肝胰腺中的相对表达量最高,且显著高于其它组织(P0.05)。低盐度胁迫显著改变了Pt CLIC基因在三疣梭子蟹鳃和肝胰腺中的表达模式,整体呈先上调后下调的趋势,并发现该基因在低盐耐受和低盐敏感家系中的表达规律差异显著。研究结果表明Pt CLIC基因在三疣梭子蟹渗透压调节中发挥重要作用,可辅助三疣梭子蟹耐低盐品系的选育。  相似文献   

合浦珠母贝贝壳珍珠层EDTA可溶性基质蛋白对无定形碳酸钙稳定性的影响     

林慧娟  方东  向量  谢莉萍  王洪钟  张贵友  麻彩萍  张荣庆 《海洋科学》2014,38(5):1-7

通过红外光谱、扫描电镜、能谱等方法探讨了合浦珠母贝贝壳珍珠层EDTA可溶性基质蛋白(ESM)对无定形碳酸钙(ACC)晶型转化的影响。试验结果显示,合浦珠母贝贝壳珍珠层ESM在镁离子存在的条件下能够显著抑制ACC的转化,并减少最终诱导形成的方解石中的镁离子含量。同时,珍珠层ESM具有调节方解石晶体晶貌的作用。  相似文献   

微波消解—冷原子荧光光谱法测定植物样品中的汞     

陈海杰  于兆水  邢夏 《物探与化探》2014,(2):393-395,401

为了简化测量植物中汞含量的方法,提高灵敏度及结果的可信度,实验采用微波消解植物样品的办法,使样品经氯化亚锡还原,再用冷原子荧光测定出植物样品中汞的含量。确定了消解样品的方法和微波消解样品的条件,优化了仪器的最佳工作参数。方法检出限0.30×10-9(稀释因子100倍),精密度(n=12)在2.09%~6.05%,加标回收率94.1%~105.5%,方法经生物国家一级标准物质验证方法准确可靠。  相似文献   

Experiment and Pitzer Model Prediction for the Solid + Liquid Equilibria of the Ternary System LiCl–CaCl2–H2O     

MENG Lingzong  LI Dan  DENG Tianlong  GUO Yafei 《《地质学报》英文版》2014,88(Z1):356-358

正1 Introduction Lithium resources are widely distributed in the oilfield brine from the Nanyishan district in the Qaidam Basin(Fan et al.,2007).The investigation of the thermodynamics and phase diagram of the brine system is valuable in providing the theoretic foundation and scientific guidance in the comprehensive exploitation of the mixture salts effectively.Comprehensive  相似文献   

273.15 到 308.15 K下二元体系（LiCl-H2O 和 MgCl2-H2O）和三元体系（LiCl-MgCl2-H2O）的体积性质研究          下载免费PDF全文

戈海文  杨红军  王敏 《盐湖研究》2022,30(4)

用高精度振动管在 273.15 至 308.15 K 的温度范围内以 5 K 的间隔对两个二元系统（LiCl-H2O 和 MgCl2-H2O）和三元系统（LiCl-MgCl2-H2O）的密度进行了实验测定密度计。基于 Vogel-Fulcher-Tamman (VFT) 方程,对 LiCl(aq) 和 MgCl2(aq) 密度与温度和摩尔浓度的相关方程的系数进行了参数化。 Young的理想混合规则被成功地应用于基于相关的二元解性质关联三元系统的密度。根据二元和三元体系的体积特性数据,根据 Pitzer 离子相互作用理论,得到 Pitzer 单盐参数和混合离子相互作用参数。在本工作中确定了三元体系在恒定离子强度下的混合体积 (ΔVm)。  相似文献   

三元体系CaCl₂?SrCl₂?H₂O的相平衡及其在钙锶分离中的应用          下载免费PDF全文

韩海军  吉祥  陈烽雄  李家乐  黄军榜 《盐湖研究》2022,30(4)

采用等温溶解平衡法研究了三元体系CaCl₂?SrCl₂?H₂O温度从323.15 K到353.15 K范围内的相平衡。基于该三元体系多温下的相平衡数据,设计开展了实验室模拟南翼山油田卤水中氯化钙和氯化锶在温度323.15 K下的分离实验。实验室模拟钙锶分离实验结果表明,在323.15 K下时分离一次固相产品中的钙锶摩尔比可以从25.46:1降至1.73:1,SrCl₂的回收率为52.47 %,所得一次固相产品在323.15 K下溶解并再次蒸发。钙和锶的摩尔比从1.73:1降至1.1:1,SrCl₂的回收率为78.67 %。  相似文献   

钢筋混凝土结构电渗阻锈技术研究   总被引：5，自引：0，他引：5  

唐军务  李森林  蔡伟成  胡明  朱雅仙 《海洋工程》2008,26(3)

通过测量电渗阻锈过程中阻锈剂渗入量、脱盐率以及钢筋极化电阻,研究了混凝土技术参数和电渗电量对电渗阻锈效果的影响.试验结果表明,电渗阻锈技术能在脱除盐污染混凝土中氯离子的同时,将有效阻锈基团快速迁移到钢筋表面,使已腐蚀的钢筋快速恢复钝化而达到防腐的目的.  相似文献   

Peat porewater chloride concentration profiles in the Everglades during wet/dry cycles from January 1996 to June 1998: field measurements and theoretical analysis     

Michael M. Reddy  Micaela B. Reddy  Kenneth L. Kipp  Aaron Burman  Paul Schuster  Peter S. Rawlik Jr. 《水文研究》2008,22(11):1713-1724

Water quality is a key aspect of the Everglades Restoration Project, the largest water reclamation and ecosystem management project proposed in the United States. Movement of nutrients and contaminants to and from Everglades peat porewater could have important consequences for Everglades water quality and ecosystem restoration activities. In a study of Everglades porewater, we observed complex, seasonally variable peat porewater chloride concentration profiles at several locations. Analyses and interpretation of these changing peat porewater chloride concentration profiles identifies processes controlling conservative solute movement at the peat–surface water interface, that is, solutes whose transport is minimally affected by chemical and biological reactions. We examine, with an advection–diffusion model, how alternating wet and dry climatic conditions in the Florida Everglades mediate movement of chloride between peat porewater and marsh surface water. Changing surface water–chloride concentrations alter gradients at the interface between peat and overlying water and hence alter chloride flux across that interface. Surface water chloride concentrations at two frequently monitored sites vary with marsh water depth, and a transfer function was developed to describe daily marsh surface water chloride concentration as a function of marsh water depth. Model results demonstrate that porewater chloride concentrations are driven by changing surface water chloride concentrations, and a sensitivity analysis suggests that inclusion of advective transport in the model improves the agreement between the calculated and the observed chloride concentration profiles. Published in 2007 by John Wiley & Sons, Ltd.  相似文献   

硒(Ⅳ)-氯化四苯胂盐酸盐络合物的吸附波及其应用     

魏显有 《岩矿测试》1991,(1)

本文研究了在HCl介质中,Se(Ⅳ)-氯化四苯胂络合物的极谱行为。在0.05mol/LHCl溶液中,该络合物于-0.58V(vs.SCE)处出现灵敏的吸附波。Se的检测下限可达0.0001μg/ml。本文拟定的方法应用于天然水中痕量Se的直接测定,结果良好。  相似文献   

Petrological constraints on the formation conditions and retrograde P–T path of the Kotsu eclogite unit, central Shikoku     

M. Matsumoto  S. Wallis  M. Aoya  M. Enami  J. Kawano  Y. Seto  N. Shimobayashi 《Journal of Metamorphic Geology》2003,21(4):363-376

A largely undocumented region of eclogite associated with a thick blueschist unit occurs in the Kotsu area of the Sanbagawa belt. The composition of coexisting garnet and omphacite suggests that the Kotsu eclogite formed at peak temperatures of around 600 °C synchronous with a penetrative deformation (D1). There are local significant differences in oxygen fugacity of the eclogite reflected in mineral chemistries. The peak pressure is constrained to lie between 14 and 25 kbar by microstructural evidence for the stability of paragonite throughout the history recorded by the eclogite, and the composition of omphacite in associated eclogite facies pelitic schist. Application of garnet‐phengite‐omphacite geobarometry gives metamorphic pressures around 20 kbar. Retrograde metamorphism associated with penetrative deformation (D2) is in the greenschist facies. The composition of syn‐D2 amphibole in hematite‐bearing basic schist and the nature of the calcium carbonate phase suggest that the retrograde P–T path was not associated with a significant increase or decrease in the ratio of P–T conditions following the peak of metamorphism. This P–T path contrasts with the open clockwise path derived from eclogite of the Besshi area. The development of distinct P–T paths in different parts of the Sanbagawa belt shows the shape of the P–T path is not primarily controlled by tectonic setting, but by internal factors such as geometry of metamorphic units and exhumation rates.  相似文献