Alkaline phosphatase activity in ornithogenic soils in polar tundra     

MA DaWei  ZHU RenBin  DING Wei  SUN JianJun  LIU YaShu  SUN LiGuang 《极地研究(英文版)》2011,22(2):92-100

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Effects of heavy metal ions (Cu2+, Pb2+ and Cd2+) on DNA damage of the gills, hemocytes and hepatopancreas of marine crab, Charybdis japonica     

Luqing Pan  Na Liu  Hongxia Zhang  Jing Wang  Jingjing Miao 《中国海洋大学学报(英文版)》2011,10(2):177-184

There are rising concerns about the hazardous effects of heavy metals on the environment. In this study, comet assay and DNA alkaline unwinding assay were conducted on the tissues (gills, hepatopancreas, and hemocytes) of Charybdis japonica in order to illustrate genotoxicity of three heavy metal ions (Cu2+, Pb2+, and Cd2+) on the marine crabs C. japonica. The crabs were exposed to Cu2+ (10, 50, and 100 ?g.L?1), Pb2+ (50, 250, and 500 ?g L?1) and Cd2+ (5, 25, and 50 ?g L?1), and the tissues were sampled at days 0.5, 1, 3, 6, 9, and 15. DNA alkaline unwinding assay was used for testing the DNA single strand break in gills and hepatopancreas and comet assay was employed for testing the DNA damage in hemocytes. The results showed that the DNA damage (F-value) of gills in the crabs exposed to the three heavy metals was decreased gradually during the exposure periods and there was a dose-time response relationship in certain time, suggesting that the levels of DNA single strand break in all the experimental groups increased significantly compared to the controls. Changes of F-value in hepatopancreas of the crabs exposed to the three heavy metals were similar to those in gills except that the peak values were found in the 500 ?g L?1 Pb2+ treatment group at day 3 and the 50 ?g L?1 Cd2+ treatment group at day 9. The ranks of DNA damage in gills and hepatopancreas induced by the three heavy metal ions (50 ?g L?1, day 15) were Cd2+ >Pb2+ >Cu2+ and Pb2+ >Cu2+ >Cd2+. The levels of DNA damage in gills were higher than those in hepatopancreas in the same experimental group. It can be concluded that indices of DNA damage can be used as the potential biomarkers of heavy metal pollution in marine environment.  相似文献   

Petrochemistry of subvolcanic dike swarms associated with the Golden Revenue Au–Cu and the Stoddart Mo–Cu ± W mineralizations (Dawson Range, Yukon Territory, Canada) and implications for ore genesis     

Thierry Bineli Betsi  David Lentz 《Ore Geology Reviews》2011,39(3):134-163

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新疆东准噶尔老鸦泉富碱花岗岩型锡矿床地质及成矿流体   总被引：3，自引：2，他引：1  

王莉娟  王京彬  王玉往  龙灵利  唐萍芝 《岩石学报》2011,27(5):1483-1492

老鸦泉碱性花岗岩位于新疆北部东准噶尔地区。老鸦泉碱性花岗岩体及其内卡姆斯特、干梁子锡矿床的矿石和岩石的岩矿鉴定、稀土元素以及流体包裹体的系统研究表明,老鸦泉碱性花岗岩及其内的花岗斑岩及含矿石英岩、云英岩化锡矿体、石英脉锡矿体,实际上是富碱花岗质岩浆逐渐分异演化的同源和最终产物,锡成矿流体为中-高温、低盐度。碱性岩浆晚期分异的大量气水热液富锡、富硅、富碱、富含F、Cl、SO²₄离子及离子团,其氧逸度高、酸度高、温度高,这种热液引起花岗岩体的硅化、云英岩化等自变质作用,在该作用中随温度、压力的降低及CH₄等还原性气体及CO₂气体的逃逸,改变了成矿流体的氧化-还原环境,流体向相对还原及碱性条件转化,在新的氧化还原、酸碱度界面条件下,其携带的锡的络合物不稳定而分解,锡沉淀成矿。  相似文献   

辽西建昌碱性粗面岩的年代学及岩石成因研究   总被引：1，自引：0，他引：1  

冯光英  刘燊  钟宏  冯彩霞  齐有强  杨毓红  杨朝贵 《矿物学报》2011,31(3):380-390

义县组火山岩在辽话分布广泛,研究区碱性粗面岩位于建昌地区义县组火山岩的顶部,详细的年代学及地球化学研究表明,锆石u—Pb年龄为（120．3±1．5）Ma,具有较高的SiO2：和碱含量,ω（SiO2）=63．52％～65．94％,ω（Na20＋K2O）=9．63％一10．15％,ω（Na20）／ω（K20）为0．96～1．08,属于高钾碱性岩系列,同时具有较低的ω（MgO）（0．75％～1．05％）,稀土配分模式为轻稀土富集,重稀土亏损的右倾型模式（La／Yb）n=8．96～12．02）,较弱的正Eu异常（δEu=1．07—1．16）,相容元素Cr（1．46×10^6～4．15X10^6）、c0（19．8X10^6。～45．9X10^6）和Ni（0．100x10^6～1．55×10^6）含量低。微量元素蛛网图显示明显的Ba和Pb正异常,以及Sr、Ti和Y负异常,此外,zr和Hf显示一定的正异常。认为碱性岩形成于拉张的构造环境,且来源深度较大,是壳幔相互作用的结果,研究区火山岩是经过强烈橄榄石、斜方辉石和单斜辉石结晶分异的富集地幔岩浆上升底侵至下地壳,并经历同化混染和部分熔融,然后沿着深大断裂喷发至地表。  相似文献   

产复合酶菌株Pseudomonas sp.NJ197产酶发酵条件的研究   总被引：2，自引：0，他引：2  

张金伟  曾润颖 《台湾海峡》2005,24(4):520-525

从南极普利兹湾深海900m的沉积物中筛选到一株同时产多种低温复合酶的菌 株NJ197,作者对其进行碳源、氮源、无机盐、起始pH值、培养时间、接种量等发酵条 件的优化实验．实验结果证明,在以0．5％可溶性淀粉为碳源,以0．5％豆饼粉为氮 源,无机盐:0．424％NH4H2PO4、0．075％K2HPO4、0．02％MgSO4、0．5％CaCO3,起始 pH值8．5,接种36h种龄的种子10％,20℃、250r／min的条件下在旋转摇床中培养 72h,产复合酶菌株产脂肪酶和淀粉酶活性最高．最佳的生理条件下复合酶中的碱性 脂肪酶和淀粉酶的产量分别提高到22．4U／cm3和30．9U／cm3,该产复合酶菌株可以 作为诱变育种、基因工程菌改造的出发菌株,在洗涤和制革工业中有良好的应用前 景．  相似文献   

氯化物型盐碱地池塘底泥中水溶性盐的分布特征     

张美昭  张兆琪  郑伟刚  董双林 《海洋湖沼通报》2001,(4):50-55

1997－1999年夏天，对位于黄河下游的氯化物型盐碱地池塘底泥中的水溶性盐进行了分析。结果表明：底泥中的含盐量，SO^2-4,Ca^2 ,Mg^2 含量是随着塘龄的增加而升高，而其余离子基本不随塘龄的增加而变化。各离子的垂直分布为“泥－土”混合层的含量较低，“水－泥”交界层含量较高。  相似文献   

Organic matter origin and distribution in suspended particulate materials and surficial sediments from the western Adriatic Sea (Italy)   总被引：5，自引：2，他引：5  

T. Tesi  S. Miserocchi  M.A. Goi  L. Langone  A. Boldrin  M. Turchetto 《Estuarine, Coastal and Shelf Science》2007,73(3-4):431-446

In this study, organic carbon (OC), total nitrogen (TN), stable carbon isotopic (δ¹³C_OC) and CuO reaction product compositions were used to identify the sources of organic matter (OM) and to quantify the relative importance of allochthonous and autochthonous contributions to the western Adriatic Sea, Italy. Suspended particulate material (195 samples) and surficial sediments (0–1 cm, 70 samples) from shallow cross-shelf transects were collected in February and May 2003, respectively. Vertical water column profiles were acquired along the same transects. Data include depth, potential temperature, salinity, density and chlorophyll fluorimetry.Along the western Adriatic shelf in the near-shore region, the phytoplankton growth was influenced by dynamics of the buoyant plumes from the Po and Appennine rivers. A small amount of very fine terrigenous material remained suspended within the coastal current and was exported southward along the shelf to the slope. High variability in the bulk composition was detected in the Po prodelta surficial sediments, whereas the western Adriatic shelf, although a larger area, exhibited a narrower range of values.A significant decoupling was observed between suspended particles in the water column and surficial deposits. The organic material collected in the water column was compositionally heterogeneous, with contributions from marine phytoplankton, riverine–estuarine phytoplankton and soil-derived OM. Frequent physical reworking of surficial sediments likely leads to the efficient oxidation of marine OC, resulting in the observed accumulation and preservation of refractory soil-derived OC delivered by the Po and Appennine rivers.  相似文献   

长牡蛎碱性磷酸酶的分离纯化及部分性质研究   总被引：5，自引：1，他引：5  

李兴暖  韩雅莉  肖湘  邓瑞鹏  周小朋  管云雁  黄长江 《台湾海峡》2003,22(4):482-486

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.  相似文献   

碱厂废水光谱特性实验研究     

张绪琴  吴隆业  张世魁  吴曙初  李武 《中国海洋大学学报(自然科学版)》2001,31(4):600-604

该文报道碱厂废水随浓度变化的光谱实验。分析碱厂废水的光谱特征 ,并与相同浓度的海水悬浮泥沙水体的光谱进行比较 ;确定该废水特征波长为 50 1.3nm。根据光谱实验结果 ,结合当前已有的水色传感器的资料 ,选择两个卫星传感器波段 :Sea WIFS第四频道 (497.2～ 52 1.6 nm)和TM第二频道 (518.9～ 6 0 0 .2 nm) ;利用数字对 (R,c) ,建立了反射率与该污染水体浓度间的相关模式。为遥感技术大面积、快速地监测碱厂废水污染水体提供理论依据。  相似文献