Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析          下载免费PDF全文

武翠玲  宋英达  高慧芳  张廉芸  任晨霞 《盐湖研究》2020,28(1)

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性，属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高，α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高，且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸，并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示，α螺旋和β片层围成了蛋白酶的催化腔，EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体，且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性，并有助于其催化功能的发挥，为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。  相似文献   

海南省农村多维贫困及影响因素的空间分异     

张金萍  林丹  周向丽  余珍鑫  宋伟  程叶青 《地理科学进展》2020,39(6):1013-1023

贫困具有多维属性,根据不同社会群体和背景从多维视角定义贫困已成为贫困问题研究的共识。依据Alkire-Foster多维贫困框架,拓展精准扶贫的“两不愁,三保障”识别标准,建立了涵盖教育、健康、居住、生活和收入指标的海南省农户多维贫困评估指标体系,基于海南省70个乡镇、134个贫困村3924户入户调查数据,采用双重临界值法评估了农户及村域多维贫困状况,进而运用地理加权回归(Geographically Weighted Regression,GWR)模型,分析了村域多维贫困影响因素的空间分异。结果显示,调查农户多维贫困率达18.22%,多维贫困程度严重的村多维贫困发生率不一定高,“两不愁、三保障”及收入指标对多维贫困指数的贡献率低。中、西部连片贫困地区多维贫困主要表现为较差的资产状况、不清洁的炊事燃料、较高的家庭成员患病率和较低的家庭成员最高学历。GWR模型分析表明,作为多维贫困最重要的影响因素,户主性别、户主受教育水平、女性劳动力占比和抚养比4个变量估计系数的空间分异明显。总体上,女性户主和低学历户主为主的地区倾向于更易发生多维贫困,二者的影响分别表现为从东到西、从北到南有所增强。女性劳动力占比为负向影响,抚养比为正向影响,呈现出自北向南增强的趋势,体现了海南贫困地区劳动力弱、女性相对更勤劳等典型地域特征。  相似文献   

阿拉尔河悬浮物对铀的吸附影响研究          下载免费PDF全文

钟翼  许建新  韩积斌  徐凯 《盐湖研究》2020,28(1):112-122

为了解阿拉尔河悬浮物对铀的吸附特性,通过静态吸附实验,研究了吸附时间、pH值、温度和铀初始浓度等因素对模拟含铀水中U(VI)去除率的影响,并从热力学和动力学方面对吸附过程进行了分析。结果表明,在T=25℃,溶液初始pH=7,接触时间为16 h时,悬浮物对铀的平衡吸附率最佳,为95.48%。随着铀初始浓度的增加,吸附量增加,但吸附率随之下降,升高温度有利于铀的吸附。铀在悬浮物上的吸附过程符合Langmuir等温吸附方程,说明悬浮物对铀为单分子层吸附,且化学吸附占主导地位。吸附动力学过程可用准二级吸附动力学模型描述,表明吸附主要受动力学控制,由两个以上步骤共同控制。FTIR和EDS分析结果表明,吸附过程中铀主要与悬浮物表面活性基团螯合并以表面络合吸附为主。吸附前后的能谱对比分析表明,吸附过程中存在离子交换行为。因此,悬浮物对铀的吸附机理是以表面络合吸附和离子交换为主、物理吸附为辅的混合吸附过程。  相似文献   

全球粮食安全格局的时空演化     

蔡建明  马恩朴  林静  廖柳文  韩燕 《地理学报(英文版)》2020,30(2):179-196

Food security is the primary prerequisite for achieving other Millennium Development Goals(MDGs).Given that the MDG of“halving the proportion of hungers by 2015”was not realized as scheduled,it will be more pressing and challenging to reach the goal of zero hunger by 2030.So there is high urgency to find the pattern and mechanism of global food security from the perspective of spatio-temporal evolution.In this paper,based on the analysis of database by using a multi-index evaluation method and radar map area model,the global food security level for 172 countries from 2000 to 2014 were assessed;and then spatial autocorrelation analysis was conducted to depict the spatial patterns and changing characteristics of global food security;then,multi-nonlinear regression methods were employed to identify the factors affecting the food security patterns.The results show:1)The global food security pattern can be summarized as“high-high aggregation,low-low aggregation”.The most secure countries are mainly distributed in Western Europe,North America,Oceania and parts of East Asia.The least secure countries are mainly distributed in sub-Saharan Africa,South Asia and West Asia,and parts of Southeast Asia.2)Europe and sub-Saharan Africa are hot and cold spots of the global food security pattern respectively,while in non-aggregation areas,Haiti,North Korea,Tajikistan and Afghanistan have long-historical food insecurity problems.3)The pattern of global food security is generally stable,but the internal fluctuations in the extremely insecure groups were significant.The countries with the highest food insecurity are also the countries with the most fluctuated levels of food security.4)The annual average temperature,per capita GDP,proportion of people accessible to clean water,political stability and non-violence levels are the main factors influencing the global food security pattern.Research shows that the status of global food security has improved since the year 2000,yet there are still many challenges such as unstable global food security and acute regional food security issues.It will be difficult to understand these differences from a single factor,especially the annual average temperature and annual precipitation.The abnormal performance of the above factors indicates that appropriate natural conditions alone do not absolutely guarantee food security,while the levels of agricultural development,the purchasing power of residents,regional accessibility,as well as political and economic stability have more direct influence.  相似文献   

Theory and measurement model of administrative region potential from a perspective of administrative division adjustment: Taking Chongqing city as a case study     

Wang  Kaiyong  Wang  Fuyuan 《地理学报(英文版)》2020,30(8):1341-1362

Journal of Geographical Sciences - There is a lack of basic theory and method to examine the effect of administrative division (AD) adjustment on the regional development. Based on the theory and...  相似文献   

Faecal indicator bacteria in New Zealand freshwater fish: a pilot study     

Sarah Coxon  Jon S Harding  Brent Gilpin 《新西兰海洋与淡水研究杂志》2019,53(3):470-479

Recent studies have suggested that poikilothermic animals, such as fish, may represent a previously overlooked source of the faecal indicator bacteria (FIB) used for the assessment of water quality. However, quantitative studies of FIB in poikilotherms are scarce. We investigated the presence of FIB in the faeces of five freshwater fish species. E. coli and enterococci were detected in 71 and 76% of faecal samples, respectively. Concentrations were highly variable, with maximum concentrations (2.1?×?10⁴ E. coli and 1.3?×?10⁶ enterococci per gram of faecal material) up to four orders of magnitude higher than present in the water column. FIB were not detected in faecal samples from marine fish. Our findings suggest that FIB ingested from the environment may be capable of replication within the fish gut, making fish a potential source and transport mechanism for FIB in New Zealand freshwaters. This may have implications for water quality monitoring.  相似文献   

基于回归模型的热带气旋条件下的海表降温参数化方案设计     

韦骏  刘欣  蒋国庆 《海洋学报(英文版)》2018,37(1):1-10

Combining a linear regression and a temperature budget formula, a multivariate regression model is proposed to parameterize and estimate sea surface temperature(SST) cooling induced by tropical cyclones(TCs). Three major dynamic and thermodynamic processes governing the TC-induced SST cooling(SSTC), vertical mixing, upwelling and heat flux, are parameterized empirically using a combination of multiple atmospheric and oceanic variables:sea surface height(SSH), wind speed, wind curl, TC translation speed and surface net heat flux. The regression model fits reasonably well with 10-year statistical observations/reanalysis data obtained from 100 selected TCs in the northwestern Pacific during 2001–2010, with an averaged fitting error of 0.07 and a mean absolute error of 0.72°C between diagnostic and observed SST cooling. The results reveal that the vertical mixing is overall the pre dominant process producing ocean SST cooling, accounting for 55% of the total cooling. The upwelling accounts for 18% of the total cooling and its maximum occurs near the TC center, associated with TC-induced Ekman pumping. The surface heat flux accounts for 26% of the total cooling, and its contribution increases towards the tropics and the continental shelf. The ocean thermal structures, represented by the SSH in the regression model,plays an important role in modulating the SST cooling pattern. The concept of the regression model can be applicable in TC weather prediction models to improve SST parameterization schemes.  相似文献   

鄂尔多斯盆地宜川地区二叠纪孢粉化石组合特征          下载免费PDF全文

高存山 《山东国土资源》2018,34(11)

通过对延长油矿延223井晚古生代孢粉组合研究以及古植被的恢复,探讨了该区晚古生代孢粉植物化石组合的地层学和古气候意义。根据孢粉化石主要属种的时代分布及其在各样品中百分含量的规律性变化,自下而上建立了3个孢粉组合:Gulisporites cochlearius-Laevigatosporites minimus组合、Sinulatisporites-Florinites组合和Lueckeisporites permianus-Platysaccus radialis组合。根据对划分孢粉组合的特征分析,通过与邻区及华北地区晚古生代孢粉组合的对比,探讨了孢粉组合的时代意义。通过孢粉组合,推测了相应时期的古植被类型,并与前人在相同地质时期所建立的大植物化石带进行了对比,分析了各地质时期植被的兴起、发展、繁盛以及衰退绝灭的历史过程,研究区二叠纪植物群属于典型的华夏植物群,反映了一种温暖潮湿的热带雨林气候,二叠纪晚期随着松柏类植物所占比例的增加,气候有变干旱的趋势。  相似文献   

帕米尔高原Rushan-Pshart古特提斯洋或中特提斯洋争论的岩石地球化学证据     

徐天德  王世锋  汤文坤  姚鑫 《地质科学》2020,(1):218-233

帕米尔高原上广泛分布的加里东期火山岩,印支期火山岩与燕山期火山岩被认为是块体依次向北俯冲拼贴到欧亚板块上的产物。特别是燕山期火山岩被认为是Rushan-Pshart中特提斯洋闭合的产物。但近年来地层古生物、火山岩证据不断表明Rushan-Pshart洋闭合时间在晚三叠一早侏罗世,由此限定Rushan-Pshart古特提斯洋性质,而帕米尔高原上广泛分布的燕山期火山岩是更南部的Shyok中特提斯洋闭合的产物。目前,中国境内Rushan-Pshart缝合带属性的研究工作展开较晚,研究程度较低。我们对塔什库尔干明铁盖沟一线燕山期火山岩带展开的工作发现零星分布的印支期花岗岩。印支期花岗岩错石U・Pb年龄显示岩体侵位时间在201 Ma左右。全岩主量元素特征表明岩石为I型高钾钙碱性闪长花岗岩;稀土元素在球粒陨石标准化图解中呈轻稀土元素相对富集,重稀土元素相对亏损的右倾海鸥型。明铁盖岩体的微量元素显示大离子亲石元素明显富集,而高场强元素明显亏损。稀土微量元素特征倾向花岗岩属性为岛弧型花岗岩,Sr-Nd-Hf同位素比值特征显示岩体形成于下地壳部分熔融环境。岩体的地化特征表明岩体形成的构造环境为板块汇聚的洋壳俯冲阶段,结合区域地质特征,我们将花岗岩体归为Rushan-Pshart古特提斯洋壳俯冲消减的产物。Rushan-Pshart缝合带传统上认为是中特提斯带,近年的研究进展认为其为古特提斯缝合带,本文的工作支持这种观点。Rushan-Pshart古特提斯缝合带的确立对帕米尔高原与青藏高原主体的块体对比提供了可信的对比方案,并对青藏高原新生代陆内变形方式的争论提供了可靠的证据。  相似文献   

Physico-chemical,bacteriological and health hazard effect analysis of the water in Taladanda Canal,Paradip area,Odisha, India          下载免费PDF全文

Rabiranjan Prusty  Trinath Biswal 《地下水科学与工程》2020,8(4):338-348

The physico-chemical and bacteriological parameters of the water in Taladanda canal and associated water-borne diseases,from which the dwellers have suffered,were studied,by using statistical method. Overuse and the addition of the wastes with sources from urbane industrial sectors,as well as the decrease in water level have caused the canal water quality declined drastically and subsequently led to extensive eutrophication and bacterial contamination. According to the water sample analytical results,the water is lightly acidic with the pH value of 4.5~6.7. The measured indexes,such as total dissolved solid (TDS),electrical conductivity (EC),total suspended solid (TSS),Mn,Zn,Al,Fe,Cu,Cr,and Hg etc,mostly have very high concentrations which are higher than permissible limit,indicating that the canal water is completely unsuitable for human consumption. Furthermore,the biological analysis shows that the total coliform (TC) is in the range of 45.9~30.2 in per 100 mL water in April,30.5~25.3/100 mL in July and 52.9~35.4/100 mL in December,respectively. Similarly,fecal coliform (FC) ranges from 12.8 to 10.1,10.5~7.5 and 13.1~6.4 per 100 mL water in the months of April,July and December respectively. As a result,people who use the water have suffered from different water-borne diseases. On the basis of disease data derived from hospital observations in a period of three years,there had been 4 284 people affected by different water-borne diseases from 2016 to 2018.  相似文献