基于16S rRNA 部分序列探讨部分鳚亚目鱼类的分子系统进化关系   总被引：1，自引：0，他引：1  

张源真  王伟  姜志强  张钊 《海洋科学》2012,36(9):89-95

通过PCR扩增获得了3科5属6种中国黄渤海海域的鳚亚目(Blennioidei)鱼类的线粒体16S rRNA基因序列片段约669bp碱基,结合来自 GenBank的5种鳚亚目其他科鱼类的相应基因片段,并以眼斑雪冰鱼(Chionodraco rastrospinosus)为外群,生成供系统发育分析的序列矩阵,利用MEGA 4.0软件分析序列的碱基组成、差异百分比、转换/颠换值等,应用最大简约法(MP)和邻接法(NJ)构建系统发育树.结果显示:在鳚亚目鱼类的16S rRNA 片段生成的序列矩阵中发现有碱基的插入缺失现象,共有207 bp变异位点,转换/颠换值为0.8,碱基平均差异为3.36;支持绵鳚(Enchelyopus elongates)归于鳚亚目绵鳚科(Zoarcidae),鳚(Azuma emmnion)归于鳚亚目线鳚科(Stichaeidae);方氏云鳚(Enedrias fangi)和云鳚(Enedrias nebulosus)种间遗传距离只有0.01,亲缘关系最近.  相似文献   

广东雷州地区3种滨珊瑚系统发生关系的研究     

刘皓  骆学贤  周操  刘丽 《广东海洋大学学报》2013,(4)

对雷州常见的3种滨珊瑚科珊瑚：澄黄滨珊瑚（Porites lutea）、灰黑滨珊瑚（Porites nigrescens）、普哥滨珊瑚（Porites pukoensis）,共32个样本的12S rRNA基因序列进行了PCR扩增和测序,研究其系统发生关系。结果显示,所得的3种珊瑚的12S rRNA基因长度在728～908 bp之间,G+C含量在34.3%～39.2%之间,遗传距离在0.004~0.019之间。利用邻接（NJ）法、最大似然（ML）法和不加权对群分析(UPGMA)法构建的系统进化树显示：3种珊瑚被分成了2个支系,澄黄滨珊瑚和灰黑滨珊瑚聚合在一个分支上,而普格滨珊瑚在另一支,这一研究结果与传统形态学分类研究的结果存在一定差异。  相似文献   

Culture observation and molecular phylogenetic analysis on the blooming green alga Chaetomorpha valida (Cladophorales, Chlorophyta) from China     

邓蕴彦  汤晓荣  詹子锋  滕林宏  丁兰平  黄冰心 《中国海洋湖沼学报》2013,31(3):552-559

The marine green alga Chaetomorpha valida fouls aquaculture ponds along the coastal cities of Dalian and Rongcheng, China. Unialgal cultures were observed under a microscope to determine the developmental morphological characters of C. valida. Results reveal that gametophytic filaments often produce lateral branches under laboratory culture conditions, suggesting an atypical heteromorphic life cycle of C. valida between unbranched sporophytes and branched gametophytes, which differs from typical isomorphic alternation of Chaetomorpha species. The shape of the basal attachment cell, an important taxonomic character within the genus, was found variable depending on environmental conditions. The 18S rDNA and 28S rDNA regions were used to explore the phylogenetic affinity of the taxa. Inferred trees from 18S rDNA sequences revealed a close relationship between C. valida and Chaetomorpha moniligera. These results would enrich information in general biology and morphological plasticity of C. valida and provided a basis for future identification of green tide forming algae.  相似文献   

Two new species of Vestrogothia (Phosphatocopina, Crustacea) of Orsten-type preservation from the Upper Cambrian in western Hunan, South China     

ZHANG HuaQiao  & DONG XiPing  School of Earth  Space Sciences  Peking University  Beijing  China  State Key Laboratory of Palaeobiology  Stratigraphy  Nanjing Institute of Geology  Palaeontology  Chinese Academy of Sciences  Nanjing 《中国科学D辑(英文版)》2009,(6)

Although fossils of Orsten-type preservation represented by Skaracarida and Phosphatocopina were first reported from the Middle and Upper Cambrian in western Hunan, South China in 2005, diversified phosphatocopine species have never been appropriately described and elucidated in terms of their evolutionary relationships. Here, we described two new species of Phosphatocopina, Vestrogothia anterispinata sp. nov. and V. bispinata sp. nov. The evolutionary relationship among all the valid species of Phosphatoco...  相似文献   

柳珊瑚分子系统发育学的研究进展   总被引：5，自引：2，他引：3  

黄晖  何建国  李秀保  张俊彬  邹仁林 《海洋通报》2005,24(2):63-71,78

珊瑚分子系统发育学研究始于90年代初,用于柳珊瑚分子系统学研究的主要分子标记是线粒体DNA和核糖体RNA。本文在重点介绍了柳珊瑚种群遗传多样性和柳珊瑚种上阶元系统发育学两方面的研究进展和所取得研究成果的同时,也讨论了珊瑚虫纲的系统发生关系。最后在简单引述了柳珊瑚分子系统发育学同传统形态分类学方法之间关系的同时,也对柳珊瑚分子系统发育学今后的重点研究方向做了预测。  相似文献   

Spatial variation of abundant picoeukaryotes in the subtropical Kuroshio Current in winter     

Ya-Fan Chan  Chih-Ching Chung  Gwo-Ching Gong  Ching-Wei Hsu 《Marine Ecology》2020,41(1):e12579

To understand the importance of picoeukaryotes in the biogeochemical cycle in the subtropical Kuroshio Current, a year-round survey of the hydrography and the distribution of picoeukaryotes were conducted in four oceanographic cruises from October 2012 to July 2013. In comparison with other seasons, the highest abundancy of photosynthetic picoeukaryotes, with concentrations >10⁴ cells/ml, was observed around the eastern boundary of the Kuroshio in the winter. Accordingly, the composition of picoeukaryotes in this cold season was further studied by a metabarcoding analysis of the 18S rRNA gene. The majority of picoeukaryotes comprised Alveolata, followed by Haptophyta and Stramenopiles. Their composition was diverse in the waters affected by the Kuroshio and in the offshore province. For Haptophyta, in contrast to clade A prevailing in the Kuroshio waters, clade B1, which was considered the host of uncultivated diazotrophic cyanobacterium group A (UCYN-A), appeared only in the offshore area. Similarly, in Stramenopiles, Pseudo-nitzschia spp. and MAST-1D, respectively, dominated in the Kuroshio-influenced and offshore areas. While Alveolata was the most abundant group, the distributions of all lineages were similar. The association between picoeukaryote succession and hydrographic change is yet to be fully understood. Our results will assist future studies on the community composition of picoplankton and their relationship with marine ecology in the region.  相似文献   

汾河太原河段水华藻类分类及分子系统研究     

王捷  石瑛  刘琪  李砧  张猛  谢树莲 《湖泊科学》2018,30(5):1332-1342

2012-2016年,每年的春、夏、秋季对汾河太原河段进行浮游植物样品采集.通过对样品的形态观察和描述,共鉴定出5种水华优势种,5月发生的裸藻水华优势种为裸藻属的膝曲裸藻(Euglena geniculata)和血红裸藻(E.sanguinea).而7-9月发生的微囊藻水华优势种为微囊藻属的铜绿微囊藻(Microcystis aeruginosa)、挪氏微囊藻(M.novacekii)和惠氏微囊藻(M.wesenbergii).分离纯化共得到11株单克隆水华藻,其中铜绿微囊藻8株,挪氏微囊藻2株,血红裸藻1株.运用cpcBA-IGS、gyrB和cpSSU基因序列构建分子系统发育树,进一步确定水华藻的系统分类地位,结果表明cpcBA-IGS是研究汾河太原河段铜绿微囊藻分类很好的分子标记,而cpSSU基因可很好地区分血红裸藻和其他裸藻种.  相似文献   

A New Family of Galeaspids (Jawless Stem-Gnathostomata) from the Early Silurian of Chongqing, Southwestern China     

CHEN Yang  GAI Zhikun  LI Qiang  WANG Jianhu  PENG Lijian  WEI Guangbiao  ZHU Min 《《地质学报》英文版》2022,96(2):430-439

A new genus and species of agnathan Eugaleaspidiformes (Galeaspida), Yongdongaspis littoralis gen. et sp. nov., is described from the Llandovery (lower Silurian) Huixingshao Formation at Yongdong Town, Xiushan County, Chongqing, southwestern China. This new Telychian taxon morphologically exhibits some transitional states between Sinogaleaspidae and a cluster of higher eugaleaspidiforms containing Tridensaspidae, Eugaleaspidae, Yunnanogaleaspis, and Nochelaspis, which we term here as the ‘eugaleaspid cluster’. Phylogenetic analysis of an extended character matrix of Galeaspida reveals Yongdongaspis, on which Yongdongaspidae fam. nov. is erected, as the sister taxon of this ‘eugaleaspid cluster’, supported by two synapomorphies, the presence of one median transverse canal, and two lateral transverse canals leaving from the infraorbital canal. As the first fish described from the Llandovery Huixingshao Formation in Chongqing, Yongdongaspis provides new fossil evidence for the subdivision and correlation of the Upper Red Beds in South China.  相似文献   

基于ND1基因序列的16种胡椒鲷鱼类分子系统进化关系研究     

梁日深  梁志刚  方浩航  谭佳瑜  彭颖暄  薛涛 《海洋学报》2014,36(10):25-32

本研究基于线粒体DNA分子标记NADH脱氢酶亚基1基因(ND1)部分序列分析了印度-西太平洋海域的胡椒鲷属及少棘胡椒鲷属共16种胡椒鲷鱼类的系统进化关系。采用最大简约法及最大似然法构建了系统进化树。进化树上,16种胡椒鲷鱼类共形成两个形态特征截然不同的类群,其中类群一的种类体表具有各种鲜艳的颜色与多变的斑纹,主要分布在西太平洋地区;类群二的种类颜色灰暗单一,少数体表具有暗色斑点与条纹,大多数分布在印度洋地区。此外,进化树上,少棘胡椒鲷属的种类都位于胡椒鲷属的类群一内部,与斑胡椒鲷,暗点胡椒鲷形成的姐妹种有较近的亲缘关系。基于遗传距离的数据,少棘胡椒鲷属与胡椒鲷属间的平均遗传距离小于胡椒鲷属内部种间的遗传距离,显示出少棘胡椒鲷属与胡椒鲷属有非常近的亲缘关系,支持少棘胡椒鲷归类于胡椒鲷属的观点。  相似文献   

东海藻华高发区塔玛亚历山大藻(Alexandrium tamarense)的形态、分子及分布特征     

李冬融  陆斗定  戴鑫烽  何飘霞  夏平  王红霞 《海洋与湖沼》2014,45(6):1241-1250

本文对分离自我国东海藻华高发区的亚历山大藻进行了详细的形态特征描述和分子系统进化分析,通过运用光学显微镜、荧光显微镜、扫描电镜和分子生物学方法,确定该藻株为塔玛亚历山大藻Alexarium tamarense(Lebour)Balech,并对其ITS序列进行了系统进化分析。该藻体长21—35μm,体宽20—33μm,ITS序列长度为592bp,系统发育树中与中国东海、南海两株A.tamarense以及一株代表"亚洲温带"基因型的A.catenella聚合在一起,支持率为100%,形态特征与A.tamarense基本一致,核糖体基因型则为塔玛复合种"亚洲温带"基因型。通过现场观测,研究浙南至闽北东海藻华高发区的塔玛亚历山大藻分布变化特点,在东海原甲藻藻华消散期,塔玛亚历山大藻逆势而上,其细胞密度达到1.7×104cell/L,并逐渐向沿岸靠近,这与季风转向,台湾暖流入侵及水体层化密切相关。  相似文献