海南万泉河口尖鳍鲤线粒体基因组测序与结构特征分析          下载免费PDF全文

蒙雨  宋一清  谢青颖  蔡杏伟  张清凤  董杨  李芳远  谢松光  申志新 《海洋科学》2023,47(11):66-78

为了解海南尖鳍鲤(Cyprinus acutidorsalis)线粒体基因组结构特征及其与鲤科(Cyprinidae)鱼类的进化关系, 本文通过二代测序获得海南万泉河口尖鳍鲤线粒体基因组全序列, 并对结构特征进行分析, 结果显示具有典型的环状闭环双链分子, 全长16 581 bp, 碱基组成为A (31.96%)、G(15.69%)、C(27.53%)和T(24.82%), 包含13个蛋白质编码基因(protein-coding genes, PCGs)、2个rRNA基因、22个tRNA基因和1个可变控制区D环。对碱基含量分析发现尖鳍鲤的碱基组成中具有较高AT含量的偏向性, 13个PCGs中有不少的偏好性密码子, 如CGA(RSCU>3.506)、CCA(RSCU>2.208)等。除tRNA^Gln、tRNA^Ala、tRNA^Asn、tRNA^Cys、tRNA^Leu(CUN)、tRNA^Tyr、tRNA^Ser(UCN)、tRNA^Pro和ND6外, 其余基因的排列于H链上。比较海南与广西海域尖鳍鲤线粒体基因组发现, 两者PCGs相似性在96%~100%, ND4、CO II、Cytb基因在起始位置、长度以及起始密码子存在不同程度的差异。另外, 将与尖鳍鲤同属的的鲤(C.carpio)、鲫(C.auratus)的13个PCGs进行两两比较表明, 3种鲤属鱼类的PCGs相似性在85%~99%。通过与其他鲤科鱼类的线粒体基因组序列构建系统发育树, 发现尖鳍鲤和鲤亲缘关系最近。本研究结果有助于理解不同鲤科鱼类的亲缘关系, 为尖鳍鲤线粒体进化分析和种质资源研究提供参考依据。  相似文献   

Adaptations of araphid pennate diatoms to a planktonic existence     

Wiebe H. C. F. Kooistra  Gandi Forlani  & Mario De Stefano 《Marine Ecology》2009,30(1):1-15

Pennate diatoms are monophyletic. Their principal cell wall elements, called valves, are shaped like a ship's hull. Within the pennates, the araphids are paraphyletic; they possess rimoportulae and pore fields located at the valve apices. The pore fields exude mucilage pads with which cells attach to one another to form chains. Many taxa use the pads also for attachment to substrata. Only a few genera are truly planktonic. The main question addressed in this study is whether the planktonic lifestyle is ancestral or derived. Phylogenies inferred from nuclear SSU rDNA gene sequences of diatoms indicated that the attached lifestyle is ancestral among the araphids, whereas a typically planktonic lifestyle seems to have developed at least three times and possibly four times independently. Acquisition of a planktonic lifestyle from benthic ancestry was accompanied by a reduction in the silicification of cell-wall elements, but changes in morphological characters shared by all four clades were not detected. The reason why only three or four araphid pennate clades have adopted a planktonic lifestyle may be related to constraints associated to their sexual reproduction mode. Partner cells of opposite mating type align with one another and produce isogametes. These gametes lack flagella; they move to one another in an amoeboid fashion, which functions well on surfaces, but seems a liability in a turbulent water column. The planktonic lineages must have overcome this constraint, e.g. by sinking to the bottom, or aggregating, to perform sexual reproduction. Members of the four araphid pennate lineages are now common constituents of the plankton, suggesting that they are ecologically successful.  相似文献   

石莼目几种绿藻的ITS区和5.8S rDNA的序列及系统发育分析石莼目几种绿藻的ITS区和5.8S rDNA的序列及系统发育分析     

李艳燕  朱立静  沈颂东  吴勋建  张耀东丁兰平 《海洋学报(英文版)》2009,28(5)

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线粒体基因片段在梭子蟹系统发育及物种鉴定中的应用   总被引：3，自引：0，他引：3       下载免费PDF全文

张姝  李喜莲  崔朝霞  王鸿霞  王春琳  刘小林 《海洋科学》2008,32(4):9-18

测定三疣梭子蟹(Portunus trituberculatus)9个个体COI基因部分序列467 bp,7个个体16S rRNA基因部分序列519 bp,同时测定样品蟹1个个体COI基因部分序列468 bp.结合GenBank中收集的梭子蟹科COJ,16S rRNA两个基因所有同源序列信息,使用Kimura双参数模型采用邻接法(NJ)、最大简约法(MP)构建梭子蟹科分子系统发育树;将样品蟹测得的COI基因序列和已知鲟属的其他蟹同源序列(429 bp)进行比对分析.结果分析表明:两个基因片段序列平均碱基AT数量分数都明显高于GC数量分数;梭子蟹属与美青蟹属关系最近,蟳属应为区别于梭子蟹属、美青蟹属、青蟹属的另一支,支持蟳属应划分在短桨蟹亚科的观点;根据遗传距离以及转换/颠换(R)值分析,判定出样品蟳为锐齿蟳.本试验运用线粒体基因片段探讨了梭子蟹类的系统发育关系以及样品蟹的种类鉴定,为线粒体基因片段在梭子蟹的物种鉴定和系统发育重建中的开发和利用提供重要参考.  相似文献   

Preliminary identification of three new isolates in genus Alexandrium (Dinophyceae) from China sea area     

Fan Lijing  Sui Zhenghong  Mao Yunxiang  Guo Hao 《中国海洋大学学报(英文版)》2007,6(1):33-39

The 5.8S ribosomal DNA sequences (5.8S rDNA) and their flanking regions, internal transcribed spacer 1 and spacer 2 (ITS1 and ITS2) of three new isolates in genus Alexandrium (Alexandrium sp. qd1, Alexandrium sp. qd2, Alexandrium sp. gz) from China were amplified, sequenced, and subjected to phylogenetic analysis. Alexandrium sp. gz and Alexandrium sp. qd1 were grouped with high bootstrap values with four strains/species, i.e., A. catenella South Korea strain, A. catenella Japan strain, and two from China, Alexandrium sp. AC03 and Alexandrium sp. AN01 being proposed to be A. catenalla in a previous study. Then Alexandrium sp. gz and Alexandrium sp. qd1 were identified as Alexandrium catenella. As A. catenella was isolated from Qingdao and Guangzhou sea areas, it supposedly distributed at least in these two areas and was genetically different. Alexandrium sp. qd2 differed greatly from species in Alexandrium. It clustered with Symbiodinium californium, Symbiodinium sp. G15 and Gymnodinium sp. Zhao 01 with 100% bootstrap value; so Alexandrium sp. qd2 affiliates to genus Symbiodinium, and is probably a free-living Symbiodinium species.  相似文献   

Preliminary Identification of Three New Isolates in Genus Alexandrium(Dinophyceae) from China Sea Area     

FAN Lijing)  SUI Zhenghong)  *  MAO Yunxiang)  and GUO Hao) ) Division of Life Science  Technology  Ocean University of China  Qingdao  P. R. China ) National Marine Environmental Monitoring Center  State Oceanic Administration  Dalian  P. R. China 《中国海洋大学学报(英文版)》2007,6(1):33-39

1 Introduction Dinoflagellate Alexandrium has received intensive at-tention in recent years, as it is a valuable bioactive com-pound resource and related to harmful algal bloom (HAB)which has negative impact on marine ecology systemaquiculture, human health and tourism (Hallegraeff andBolch, 1992; Smayda, 1997; Vale and Antonia de MSampayo, 2001). However, not all of the species in genusAlexandrium produce toxin (Wendy et al., 2001), so rapididentification and clarification of the phyloge…  相似文献   

Morphology, Distribution, Life Habits and Phylogenetic Affinities of the Recent Brachiopod Gwynia capsula (Jeffreys)     

A. Logan    D. I. MacKinnon  J. E. Phorson 《Marine Ecology》1997,18(3):239-252

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Foliose Dictyoceratida of the Australian Great Barrier Reef III. Preliminary Electrophoretic Systematics     

James A. Stoddart 《Marine Ecology》1989,10(2):167-178

Abstract. Techniques of starch-gel electrophoresis were applied in a taxonomic assessment of a difficult group of dictyoceratid sponges. A phylogenetic tree derived from the resultant electrophoretic characters agreed well with a recent taxonomic revision of this group produced in a companion study. However, this must be seen as a preliminary result, as the electrophoretic character set was restricted to only three enzyme systems due to the extensive effects of a nonspecific enzyme which precluded the effective interpretation of most other enzymes assayed. This non-specific enzyme may be linked to a highly developed relationship between this group of sponges and symbiotic cyanobacteria.  相似文献   

细胞色素b基因序列与7种石首鱼类的系统进化   总被引：12，自引：0，他引：12  

田兰香  梁冰  张树义  赵盛龙  王日昕 《台湾海峡》2004,23(4):436-443

通过比较一段381bp细胞色素b基因序列，分析了石首鱼科7种石首鱼的系统发育关系．该序列有51个单变异多态位点、128个简约信息多态位点，对应的氨基酸序列有41个氨基酸变异位点．根据Kimura 2参数构建的邻接树(NJ tree)和最大简约树(MP tree)都显示同样的结果：7种石首鱼类构成一个单系群．皮氏叫姑鱼位于单系群的基部，且与其他石首鱼分离时间很早；大黄鱼和棘头梅童鱼亲缘关系最近。并与钩牙皇石首鱼和波纹短须石首鱼构成姐妹群．黄姑鱼和鱿鱼也接近树的基部．  相似文献   

Characteristics and phylogeny of light-harvesting complex gene encoded proteins from marine red alga Griffithsia japonica     

LIU Chenlin  HUANG Xiaohang  LEE Yookyung  LEE Hongkum  LI Guangyou 《海洋学报(英文版)》2005,24(2):120-130

1 Introduction T he light-harvesting com plex (LH C ) com posedofphotosynthesis pigm ents and associated proteins arelocated on the thylakoid m em brane of chloroplast inhigherplants and algae. T he capture oflightenergy isthe first step in photosynthesis and is largely accom -plished by the pigm entprotein com plexes. To m axi-m ize the capture of light energy under various envi-ronm entalconditions, the LH C s w ith differentchro-m ophore com positions have evolved in differentpho-tosynth…  相似文献