蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析   总被引：4，自引：0，他引：4  

孟学平高如承  董志国阎斌伦程汉良  李艳杰陈建安 《广东海洋大学学报》2006,26(4):8-13

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。  相似文献   

Biostratigraphic and aminostratigraphic constraints on the age of the Middle Pleistocene glacial succession in north Norfolk,UK     

Richard C. Preece  Simon A. Parfitt  G. Russell Coope  Kirsty E. H. Penkman  Philippe Ponel  John E. Whittaker 《第四纪科学杂志》2009,24(6):557-580

Considerable debate surrounds the age of the Middle Pleistocene glacial succession in East Anglia following some recent stratigraphical reinterpretations. Resolution of the stratigraphy here is important since it not only concerns the glacial history of the region but also has a bearing on our understanding of the earliest human occupation of north‐western Europe. The orthodox consensus that all the tills were emplaced during the Anglian (Marine Isotope Stage (MIS) 12) has recently been challenged by a view assigning each major till to a different glacial stage, before, during and after MIS 12. Between Trimingham and Sidestrand on the north Norfolk coast, datable organic sediments occur immediately below and above the glacial succession. The oldest glacial deposit (Happisburgh Till) directly overlies the ‘Sidestrand Unio‐bed’, here defined as the Sidestrand Hall Member of the Cromer Forest‐bed Formation. Dating of these sediments therefore has a bearing on the maximum age of the glacial sequence. This paper reviews the palaeobotany and describes the faunal assemblages recovered from the Sidestrand Unio‐bed, which accumulated in a fluvial environment in a fully temperate climate with regional deciduous woodland. There are indications from the ostracods for weakly brackish conditions. Significant differences are apparent between the Sidestrand assemblages and those from West Runton, the type site of the Cromerian Stage. These differences do not result from contrasting facies or taphonomy but reflect warmer palaeotemperatures at Sidestrand and a much younger age. This conclusion is suggested by the higher proportion of thermophiles at Sidestrand and the occurrence of a water vole with unrooted molars (Arvicola) rather than its ancestor Mimomys savini with rooted molars. Amino acid racemisation data also indicate that Sidestrand is significantly younger than West Runton. These data further highlight the stratigraphical complexity of the ‘Cromerian Complex’ and support the conventional view that the Happisburgh Till was emplaced during the Anglian rather than the recently advanced view that it dates from MIS 16. Moreover, new evidence from the Trimingham lake bed (Sidestrand Cliff Formation) above the youngest glacial outwash sediments (Briton's Lane Formation) indicates that they also accumulated during a Middle Pleistocene interglacial – probably MIS 11. All of this evidence is consistent with a short chronology placing the glacial deposits within MIS 12, rather than invoking multiple episodes of glaciation envisaged in the ‘new glacial stratigraphy’ during MIS 16, 12, 10 and 6. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

Spatial and temporal changes in microbial diversity of the Marmara Sea sediments   总被引：1，自引：0，他引：1  

Kolukirik M  Ince O  Cetecioglu Z  Celikkol S  Ince BK 《Marine pollution bulletin》2011,62(11):2384-2394

Spatial (10 different locations) and temporal (2 years) changes in characteristics of the Marmara Sea Sediments were monitored to determine interactions between the chemical and microbial diversity. The sediments were rich in terms of hydrocarbon, nitrate, Ni and microbial cell content. Denitrifying, sulfate reducing, fermentative and methanogenic organisms were co-abundant in 15 cm below the sea floor. The local variations in the sediments’ characteristics were more distinctive than the temporal ones. The sulfate and nitrate contents were the main drivers of the changes in the microbial community compositions. N and P were limited for microbial growth in the sediments, and their levels determined the total cell abundance and activity. Seasonal shifts in temperatures of the shallow sediments were also reflected in the active cell abundances. It was concluded that the Marmara Sea is a promising ecosystem for the further investigation of the ecologically important microbial processes.  相似文献   

中国大陆科学钻探（CCSD）深部地层微生物研究——两株未培养地下微生物菌株原位含量的分析   总被引：2，自引：0，他引：2  

王远亮  夏莹  东秀珠  董海良  杨克迁  董志扬  黄力 《岩石学报》2006,22(7):2103-2106

从地下1033．77米深处岩芯中提取微生物DNA,经PCR扩增,获得16S rDNA序列。选取其中两个序列(CCSD1305与CCSD1307),根据其16S rDNA可变区设计PCR引物,通过实时定量PCR分析,确定这两株未培养微生物在岩芯中的生物量。结果表明,CCSD1305与CCSD1307在该岩芯中的数量分别约为6．6×104和2．18×106个／克岩石。  相似文献   

胃肠酸度下中药及补铁制剂中铁活性的影响研究     

刘淑娟  王达成  杨沁羽 《华东地质学院学报》2006,29(2):159-163

在人体胃肠液的酸度条件下,采用正辛醇-水分配体系模拟药物在人体肠胃中的分配情况,研究了中药和补铁制剂中铁的形态及其在人体内的吸收情况,并探讨了中药不同比例的配伍、胃肠酸度及还原性物质的存在对水溶态铁和醇溶态铁的影响。结果表明,药物溶液中的铁形态与药物本身特性和配伍情况有关,酸度及配伍对药物中铁的溶出率和溶液中铁的形态有较大影响,人体对铁的吸收与胃肠酸度和还原性物质的存在有关。  相似文献   

Preparation and After-Treatment of BaMgAl10 O17 ：Mn^2＋     

王永钱  张锦华宋羽 袁曦明 《中国地质大学学报(英文版)》2006,17(3):272-275

INTRODUCTION The properties of phosphor usedin a plasma dis-play panel (PDP) affect the performance of the PDP(Okazaki et al .,2000 ; Rao and Devine ,2000) . Theluminescent properties of the phosphor are decided bythe morphology ,particle size and size distribution ofthe powder ,sothe requirements for the powder mor-phology are high in a color PDP which belongs to ahigh resolution display apparatus (I m et al .,2005 ;Yang et al ., 2005) . Studies have shown that withsmall particle si…  相似文献   

青藏高原非均匀地表区域能量通量的研究   总被引：7，自引：1，他引：7  

马耀明  仲雷  田辉  孙方林  苏中波  Massimo Menenti 《遥感学报》2006,10(4):542-547

卫星遥感在研究青藏高原非均匀地表区域能量通量和蒸发（蒸散）量时有其独到的作用。本文介绍了基于NOAA-14 AVHRR和Landsat TM资料推算藏北高原地区区域地表特征参数、植被参数及区域地表热通量的方案，并把其用于GAME／Tibet（全球能量水循环之亚洲季风青藏高原试验研究）和CAMP／Tibet（“全球协调加强观测计划（CEOP）亚澳季风之青藏高原试验研究”）试验区。并指出了此方法估算青藏高原非均匀地表区域地表能量通量和蒸发（蒸散）量时存在的难点问题和解决问题的可能途径。  相似文献   

基于NOAA/POES卫星观测的磁层相对论电子起源的初探   总被引：1，自引：0，他引：1       下载免费PDF全文

谢伦  涂蔚超  张辉  濮祖荫 《地球物理学报》2007,50(6):1632-1638

本文利用低高度极轨卫星NOAA/POES的观测数据，并结合ACE卫星和Polar卫星的观测结果，研究分析了磁层相对论电子的起源. NOAA/POES卫星对于不同地磁活动时期相对论电子的分布和起源进行了较为详细观测, 分析结果表明（1） 亚暴期间注入磁层的能量电子可以为与磁暴相关的磁层高能电子暴提供种子电子；（2）太阳质子事件期间太阳风中的能量电子也可以为磁层中的相对论电子提供所需要的源.  相似文献   

基于人工神经网络的赤潮卫星遥感方法研究   总被引：7，自引：1，他引：7  

楼琇林  黄韦艮 《遥感学报》2003,7(2):125-130

根据赤潮的卫星遥感探测机理，应用人工神经网络技术，建立和利用NOAA AVHRR可见光和热红外波段遥感数据的BP神经网络赤潮信息提取模型。应用实例显示。基于该人工神经网络方法可以提取赤潮发生地点和范围等信息，赤潮探测正确率达到78.5%。研究结果表明，应用人工神经网络方法提取赤潮信息是可行的。本文中建立的BP赤潮信息提取模型适当修改后可移植应用于其它传感器遥感数据进行赤潮信息提取。  相似文献   

德令哈6．6级地震前卫星红外长波辐射OLR的分析   总被引：4，自引：0，他引：4  

秦松涛  孙洪斌 《高原地震》2003,15(3):42-44,51

应用NOAA卫星资料反演的地震长波辐射值OLR对2003年4月17日发生在青海省德令哈西的6．6级地震进行了追踪研究。结果显示：2003年1月起OLR距平值出现明显的高值异常区域。  相似文献