Microbial Diversity in Nankai Trough Sediments at a Depth of 3,843 m   总被引：6，自引：0，他引：6  

Lina Li  Jean Guenzennec  Peter Nichols  Pierre Henry  Miki Yanagibayashi  Chiaki Kato 《Journal of Oceanography》1999,55(5):635-642

Dense populations of bivalves, primarily Calyptogena sp., were observed at cold seeps of the Nankai Trough. Bacterial input to the sediment was estimated through determination of phospholipid ester-linked fatty acid (PLFA) and DNA profiles. Results indicated a bacterial biomass of 10⁹ cells (g dry wt)^-1 while individual fatty acid profiles revealed a predominance of monounsaturated fatty acids, mainly 18:1 isomers. The presence of these fatty acids can be interpreted to reflect a response to low temperature and a predominance of psychrophilic bacteria. DNA fragments encoding bacterial ribosomal RNA small-subunit sequences (16S rDNA) were amplified by the polymerase chain reaction method using DNA extracted directly from the sediment samples. From the sequencing results, at least 19 kinds of bacterial 16S rDNAs related to mostly the Proteobacteria and a few gram-positive bacteria were identified. These results suggest that the bacterial community in the Nankai Trough sediments consists of mainly bacteria belonging to the Proteobacteria , , and subdivisions. Bacteria belonging to the and subdivisions, which are known to include epibiont and sulfate reducing bacteria, respectively, were mostly detected in the sediment obtained from inside the area of the Calyptogena community, and the -Proteobacteria may function to supply reduced sulfur to bacterial endosymbionts of Calyptogena.  相似文献   

3种蛏类线粒体16SrRNA和COI基因片段的序列比较及其系统学初步研究   总被引：15，自引：0，他引：15  

陈丽梅  孔晓瑜  喻子牛  于珊珊  徐晖 《海洋科学》2005,29(8):27-32

对3种蛏类大竹蛏（Solen grandis），长竹蛏（Solen strictus）和小刀蛏（Cultellus attenuatus）的线粒体16SrRNA和COI基因片段序列进行了比较并对其系统学进行了初步研究。得到的序列总长度分别为472-481bp（16S）和658bp（COI）。3种蛏序列的碱基组成均显示出较高的A＋T比例（16SrRNA基因62．1％；COI基因62．8％）。对位排序比较表明，16SrRNA片段种内个体间变异较小，3种类间存在128个碱基变异位点（其中包括127个简约信息位点）和5个插入／缺失位点，总共12个碱基长；COI片段有200个碱基存在变异，其中包括191个简约信息位点，不存在任何插入／缺失位点。数据分析结果表明16SrRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0．0856和0．1712，两竹蛏类与小刀蛏的遗传距离分别为0．3054，0．2798和0．2662，0．2933。作者认为小刀蛏与竹蛏之间的遗传距离已达到科之间的水平，结果支持将其提升为刀蛏科的分类观点。3种蛏类线粒体16SrRNA和COI基因在种间明显的多态性，证实了16SrRNA和COI基因序列均普遍适用于蛏类种及以上阶元的系统学分析。  相似文献   

Effect of NOAA satellite orbital drift on AVHRR-derived phenological metrics     

《International Journal of Applied Earth Observation and Geoinformation》2017

The U.S. Geological Survey (USGS) Earth Resources Observation and Science (EROS) Center routinely produces and distributes a remote sensing phenology (RSP) dataset derived from the Advanced Very High Resolution Radiometer (AVHRR) 1-km data compiled from a series of National Oceanic and Atmospheric Administration (NOAA) satellites (NOAA-11, −14, −16, −17, −18, and −19). Each NOAA satellite experienced orbital drift during its duty period, which influenced the AVHRR reflectance measurements. To understand the effect of the orbital drift on the AVHRR-derived RSP dataset, we analyzed the impact of solar zenith angle (SZA) on the RSP metrics in the conterminous United States (CONUS). The AVHRR weekly composites were used to calculate the growing-season median SZA at the pixel level for each year from 1989 to 2014. The results showed that the SZA increased towards the end of each NOAA satellite mission with the highest increasing rate occurring during NOAA-11 (1989–1994) and NOAA-14 (1995–2000) missions. The growing-season median SZA values (44°–60°) in 1992, 1993, 1994, 1999, and 2000 were substantially higher than those in other years (28°–40°). The high SZA in those years caused negative trends in the SZA time series, that were statistically significant (at α = 0.05 level) in 76.9% of the CONUS area. A pixel-based temporal correlation analysis showed that the phenological metrics and SZA were significantly correlated (at α = 0.05 level) in 4.1–20.4% of the CONUS area. After excluding the 5 years with high SZA (>40°) from the analysis, the temporal SZA trend was largely reduced, significantly affecting less than 2% of the study area. Additionally, significant correlation between the phenological metrics and SZA was observed in less than 7% of the study area. Our study concluded that the NOAA satellite orbital drift increased SZA, and in turn, influenced the phenological metrics. Elimination of the years with high median SZA reduced the influence of orbital drift on the RSP time series.  相似文献   

南极青霉菌Penicillium sp.S-1-16摇瓶发酵条件的初步优化          下载免费PDF全文

叶科元 唐潮卢小玲  吴文惠刘小宇 《极地研究》2016,28(3):424-429

对一株具有细胞毒活性的南极青霉属真菌Penicillium sp.S-1-16进行发酵条件的初步优化。通过对基础发酵培养基、发酵温度及培养基配制用水的筛选,以次级代谢产物量为主要指标,同时参考生物量及细胞毒活性指标来进行初步优化,获得一个较优的摇瓶发酵条件:酵母提取粉0.3%,蛋白胨0.5%,麦芽糖0.3%,葡萄糖1%,蔗糖5%,自来水,初始p H=7.0,接种量15%,18℃,180 r·min-1发酵15 d。采用初步优化后的条件进行发酵,与初始发酵条件相比,其次级代谢产物量提高到568.75%,对A549肿瘤细胞的抑制作用增强至265%,并且对He La肿瘤细胞和SGC-7901肿瘤细胞的抑制率分别从0提高到48.19%和69.18%,效果显著,为获得菌株中活性次级代谢产物奠定了基础。  相似文献   

基于第二代测序技术的条斑紫菜(Pyropia yezoensis)贝壳丝状体附生菌群研究   总被引：1，自引：0，他引：1  

盖珊珊  张伟东 《海洋与湖沼》2016,47(5):990-996

条斑紫菜(Pyropia yezoensis)贝壳丝状体黄斑病给育苗产业造成危害,但目前对健康状态下的菌群结构及其发病后变化仍缺乏认识。本研究基于第二代测序技术和16S r RNA序列分析,对健康和患病丝状体的细菌组成与变化进行比较。测序共产生1.6Gb配对末端序列,获得了第一个高通量的条斑紫菜贝壳丝状体细菌群落数据集。通过数据分析共识别出7,833种可操作分类单元(operational taxonomic units,OTUs),比对确定为18个细菌门。相对于健康样本,黄斑病丝状体样本的微生物多样性显著增加。主坐标分析和非加权组平均法(unweighted pair group method using arithmetic average,UPGMA)聚类均将健康和黄斑病丝状体分成不同的组,且健康组样本间的距离明显小于黄斑病组。上述结果说明,健康贝壳丝状体保持着相似的微生物群落结构,而患病后的丝状体则发生了不同的变化;健康样本和黄斑病样本组间差异显著的细菌属有39个,其中29个在健康样本中的相对丰度明显高于病患组样本。本研究从菌群生态角度分析了健康贝壳丝状体微生物菌群结构、发病后菌群的变化及其对丝状体生长的潜在作用,为丝状体病害的检测和预防提供有价值的信息。  相似文献   

宁波沿海陆源排污口放线菌(Actinomycetales sp.)的分布特点     

司开学  夏长革  王朝阳  张迪骏  何珊  周君  张红燕  韩姣姣  崔晨茜  董丽莎  苏秀榕 《海洋与湖沼》2016,47(2):400-406

利用454高通量测序技术对宁波沿海10个陆源排污口20个站位的放线菌的时空分布及5个工业排污口的放线菌的种类作了整体分析。成功鉴定出了83个属,84个种。研究结果显示:放线菌在陆源排污口的分布呈现季节性分布,从3月份到10月份,放线菌数量呈现先升高后下降的变化,5月份和8月份数量居高,在3月份和10月份偏低;在综合排污口(S4,S6,S8和S9)检测频次较高,在工业排污口(S1,S3,S5,S7和S10)检测频次居中,在市政排污口(S2)检出频次最低。不同类型的排污口,氨氮浓度的排出量不同,放线菌的种类和数量也不同。在5个工业排污口中,S7和S10检出的共同菌最多;在S7独自检出短双歧杆菌(Bifidobacterium breve)、长双歧杆菌(B.longum)和两岐双岐杆菌(B.bifidum),表明存在粪源污染物;在S5检出皱孢链霉菌(Streptomyces scabrisporus)和硫藤黄链霉菌(S.thioluteus),显示有石油降解物和重金属的污染。总体上看,放线菌数量在距排污口外50m处略高于排污口处。排放指标越相似,菌的种类越接近。  相似文献   

大黄鱼白细胞介素17受体E-like的分子特征及进化分析          下载免费PDF全文

丁扬  敖敬群  艾春香  陈新华 《应用海洋学学报》2016,35(1):107-115

白细胞介素17受体(Interleukin 17 receptor,IL-17R)家族与IL-17细胞因子家族介导的各类反应有着密切联系.IL-17RE-like(IL-17REL)是一类最新发现的IL-17受体,其结构与IL-17RE相似,但尚未发现其配体.本研究克隆得到大黄鱼(Larimichthys crocea)IL-17REL(Lyc IL-17REL)的全长c DNA,其开放阅读框全长1 659个核苷酸,推断可编码一个由552个氨基酸组成的蛋白,含有信号肽、胞外段、跨膜区和胞内段.与其他物种的IL-17REL相似,Lyc IL-17REL蛋白的胞外段具有一个保守的FN I-like结构域,而胞内段缺少IL-17受体家族保守的SEFIR(SEF and IL-17R)结构域.多序列比对分析表明Lyc IL-17REL与日本河豚(Takifugu rubripes)IL-17REL的氨基酸序列同源性最高,序列一致性可达57.0%.系统进化分析表明,脊椎动物的IL-17受体家族可分为2类,IL-17RA/RB/RD为一类,IL-17RC/RE/REL为一类.其中鱼类及高等脊椎动物的IL-17REL与IL-17RE亲缘关系最近,并且推测二者可能是由同一基因从硬骨鱼类之前的物种中分化而来.组织分布表明Lyc IL-17REL在所有被检测的组织中为组成型表达,在黏膜相关组织如小肠和鳃中的表达量最高.大黄鱼受嗜水气单胞菌(Aeromonas hydrophila)诱导刺激后,Lyc IL-17REL在其头肾及鳃中的基因转录水平均显著上调.这表明Lyc IL-17REL可能在细菌感染引起的免疫反应中起着重要作用.  相似文献   

Hydrodynamics of photoionized columns in the Eagle Nebula, M 16     

R.J.R. Williams  D. Ward-Thompson  A.P. Whitworth 《Monthly notices of the Royal Astronomical Society》2001,327(3):788-798

We present hydrodynamical simulations of the formation, structure and evolution of photoionized columns, with parameters based on those observed in the Eagle Nebula. On the basis of these simulations we argue that there is no unequivocal evidence that the dense neutral clumps at heads of the columns were cores in the pre-existing molecular cloud. In our simulations, a variety of initial conditions leads to the formation and maintenance of near-equilibrium columns. Therefore, it is likely that narrow columns will often occur in regions with large-scale inhomogeneities, but that observations of such columns can tell us little about the processes by which they formed. The manner in which the columns in our simulations develop suggests that their evolution may result in extended sequences of radiation-induced star formation.  相似文献   

Spatial and temporal changes in microbial diversity of the Marmara Sea sediments   总被引：1，自引：0，他引：1  

Kolukirik M  Ince O  Cetecioglu Z  Celikkol S  Ince BK 《Marine pollution bulletin》2011,62(11):2384-2394

Spatial (10 different locations) and temporal (2 years) changes in characteristics of the Marmara Sea Sediments were monitored to determine interactions between the chemical and microbial diversity. The sediments were rich in terms of hydrocarbon, nitrate, Ni and microbial cell content. Denitrifying, sulfate reducing, fermentative and methanogenic organisms were co-abundant in 15 cm below the sea floor. The local variations in the sediments’ characteristics were more distinctive than the temporal ones. The sulfate and nitrate contents were the main drivers of the changes in the microbial community compositions. N and P were limited for microbial growth in the sediments, and their levels determined the total cell abundance and activity. Seasonal shifts in temperatures of the shallow sediments were also reflected in the active cell abundances. It was concluded that the Marmara Sea is a promising ecosystem for the further investigation of the ecologically important microbial processes.  相似文献   

Geomorphic expression of neotectonic activity in a low relief area in an Airborne Laser Scanning DTM: A case study of the Little Hungarian Plain (Pannonian Basin)     

Balzs Szkely  Andrs Zmolyi  Erich Draganits  Christian Briese 《Tectonophysics》2009,474(1-2):353

The NW corner of the Little Hungarian Plain, which lies at the junction of the Eastern Alps, the Pannonian Basin and the Western Carpathians, is a neotectonically active region linking the extrusional tectonics of the Eastern Alps with the partly subsiding Little Hungarian Plain. The on-going deformation is verified by the earthquake activity in the region. An extremely flat part of the area, east of Neusiedlersee, the so-called Seewinkel, has been investigated with Airborne Laser Scanning (ALS, also known as airborne LiDAR) techniques, resulting in a digital terrain model (DTM) with a 1 m grid resolution and vertical precision of better than 10 cm. The DTM has been compared with known and inferred neotectonic features.Potential neotectonic structures of the DTM have been evaluated, together with geological maps, regional tectono-geomorphic studies, geophysical data, earthquake foci, as well as geomorphological features and the Quaternary sediment thickness values of the Seewinkel and the adjacent Parndorfer plateau. A combined evaluation of these data allows several tectonic features with a relief of < 2 m to be recognized in the DTM. The length of these linear geomorphological structures ranges from several hundred meters up to several kilometers. The most prominent feature forms a 15 km long, linear, 2 m high NE–SW trending ridge with gravel occurrences having an average grain size of ca. 5 cm on its top. We conclude this feature to represent the surface expression of the previously recognized Mönchhof Fault. In general, this multi-disciplinary case study shows that ALS DTMs are extremely important for tectono-geomorphic investigations, as they can detect and accurately locate neotectonic structures, especially in low-relief areas.  相似文献