Comparison of the PCR-DGGE fingerprints in the gastrointestinal predominant bacterial community in Lutjanus sebae          下载免费PDF全文

ZHOU Zhi-gang  SHI Peng-Jun  YAO Bin  HE Su-xu  SU Yong-quan 《海洋科学》2008,32(11)

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Phylogenetic analysis of bacteria in sea ice brine sampled from the Canada Basin, Arctic Ocean     

李会荣俞勇  陈波曾胤新  任大明 《极地研究(英文版)》2006,17(2):81-89

Bacterial diversity in sea ice brine samples which collected from four stations located at the Canada Basin, Arctic Ocean was analyzed by PCR-DGGE. Twenty-three 16S rDNA sequences of bacteria obtained from DGGE bands were cloned and sequenced. Phylogenetic analysis clustered these sequences within γ-proteobacteria, Cytophaga-Flexlbacter-Bacteroides （CFB） group, Firmicutes and Actinobacteria. The phylotype of Pseudoalteromonas in the γ-proteobacteria was predominant and members of the CFB group and γ-proteobacteria were highly abundant in studied sea ice brine samples.  相似文献   

Phylogenetic analysis of cultivable bacteria isolated from Arctic sea-ice     

林学政  沈继红  黄晓航  高爱国 《极地研究(英文版)》2007,18(1):84-89

Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter.  相似文献   

Cylindrotheca closterium Is a Species Complex as Was Evidenced by the Variations of rbcL Gene and SSU rDNA     

LI Haitao YANG Guanpin SUN Ying WU Suihan ZHANG Xiufang 《中国海洋大学学报(英文版)》2007,6(2):167-174

The genus Cylindrotheca consists of a small group of marine diatoms with a few species described. Eleven isolates of diatoms identified as Cylindrotheca closterium morphologically were obtained from Jiaozhou Bay with their nuclear-encoded small-subunit ribosomal RNA （SSU rDNA） and chloroplast-encoded rbcL gene sequences determined in this study. Interestingly, very high sequence divergences of SSU rDNA and rbcL gene were found among these isolates, and numerous nucleotide variation of rbcL gene caused relatively few variation of deduced amino acid sequence. Phylogenetic analyses based on SSU rDNA and rbcL gene, respectively, grouped the isolates into 6 clades. Phylogenetic tree of SSU rDNA placed all the Cylindrotheca isolates together, separating them into two lineages clearly. LineageⅠ was composed of the eleven C. closterium isolates obtained in this study together with another C. closterium isolate, but some clades were not well supported. LineageⅠwas contained two C. closterium isolates and one C. fusiformis isolate. Phylogenetic analysis of rbcL gene also separated the Cylindrotheca isolates into two well-defined lineages. The eleven C. closterium isolates formed a lineage and all clades were supported strongly. Statistical comparisons of SSU rDNA indicated that the average distance within lineageⅠwas significantly higher than that of other microalgae species （P 〈0.01）. These results suggested the existence of cryptic species within C. closterium.  相似文献   

厦门地区近海温泉嗜热菌的分离、鉴定   总被引：1，自引：0，他引：1  

杨波  陈新华 《台湾海峡》2007,26(1):61-70

嗜热菌是一类特殊的极端环境微生物.本文所用的实验样品采集于厦门地区两个近海温泉的4个不同位点,通过16SrDNA序列分析对所分离到的312株细菌进行了归类总结,鉴定出嗜热栖热菌、嗜热脂肪泥土芽孢杆菌、嗜高温纤维分解菌、泥土芽孢杆菌属、水生栖热菌、假单胞菌属、木糖氧化无色杆菌、uncultured sheep mite bacte-rium、uncultured gamma proteobacterium等9种嗜热菌株,其中嗜高温纤维分解菌和嗜热栖热菌是主要的可培养优势菌群.其生长温度均在60-85℃,中性pH值.从而得出厦门地区近海温泉样品中的微生物生长类群信息,并发现了某些可能的新菌群,有助于了解该地区近海温泉特有环境下的可培养的微生物群落类型.  相似文献   

16S rDNA技术在近海环境监测中的应用     

衣丹  姜伟  林学政  石红旗  刘大磊 《海岸工程》2005,24(4):42-47

将16S rDNA技术应用于近海环境监测中，分析海洋环境样品中的微生物组成与环境污染程度的关系．从原核微生物多样性的改变较为准确地确定海洋污染程度，是传统海洋环境监测的良好补充，有利于加大近海海洋环境监测力度。  相似文献   

泰国近海习见有毒立方水母和钵水母的遗传分析   总被引：2，自引：0，他引：2       下载免费PDF全文

刘瑞娟  肖洁  张学雷  Charatsee Aungtonya 《海洋学报》2016,38(6):51-61

本研究利用线粒体16S rDNA和核基因18S rDNA片段,对泰国沿海常见的有毒水母进行遗传分析,并比较了2个基因片段作为通用分子标记,在研究水母类多个纲的遗传多样性中的应用。研究发现,泰国近海的有毒水母存在较高的遗传多样性,所获得的32个样品可以分为9个种,包括4种钵水母、4种立方水母和1种水螅水母。然而,完全确定各种的分类地位,还需要更多的形态、生活史等方面信息。两个基因片段均能明确区分各种类,但核基因18S序列比线粒体基因片段更为保守。根据16S基因片段序列计算水母种内和种间的K2P(Kimura 2-parameter)遗传距离,发现所研究的9个水母种类,种内遗传距离在0~0.050之间,其中94%的种内遗传距离小于0.040,同纲种间的遗传距离为0.204~0.474,其中91%的种间遗传距离大于0.250;而利用18S基因,种内距离在0~0.002之间,同纲种间距离为0.008~0.066(平均为0.038,SE=0.006)。16S的AT碱基含量明显高于核基因18S,且16S的碱基含量在不同纲之间有显著差异,进一步表明水母线粒体16S基因的突变率相对较高,适合研究水母较低分类阶元以及种下的遗传差异。  相似文献   

Depth-related distribution of bacterial community in sediments of eutrophic Guanting reservoir   总被引：1，自引：0，他引：1  

QU Jianhang  YUAN Hongli  HUANG Huaizeng  WANG Entao 《中国科学D辑(英文版)》2005,48(Z2)

In this study, DNAs were extracted from sediment samples at depths of 5, 35, and 69 cm from eutrophic Guanting reservoir, China. 16S rDNAs were amplified by PCR and clone libraries were constructed. The depth-related distribution of bacterial community in the sediment was characterized by using amplified 16S rDNA restriction analysis (ARDRA) and sequencing of the dominant clones. The results indicated that species diversity in the sediment of Guanting reservoir was rather high with the Shannon-Wiener index about 5.8. Bacterial richness varied in different depths: the highest in the sample of 35 cm in depth; followed by the sample of 5 cm in depth; and the lowest bacterial richness in the sample of 69 cm. Dominant species from the three samples were different although there were some common clones. PhyIogenetic analysis showed that all of the dominant clones in the three layers were uncultured bacteria and distantly related to the previously reported species in beta or gamma subclass of proteobacteria, including bacterial groups that have the ability to degrade aromatic hydrocarbons, n-all:anes, chlorinated organic compounds, or to accumulate polyphosphate, etc. Changes of deptt-related bacterial community in the Guanting reservoir sediment might reflect the pollution history and the water quality of the reservoir. In addition, the cloned sequences from the Guanting reservoir sediment were all different from the presently reported ones, indicating that there were some particular bacteria in that environment.  相似文献   

Chromosomal localization of the major ribosomal RNA genes in scallop Chlamys farreri   总被引：3，自引：1，他引：3  

HUANG Xiaoting  BAO Zhenmin  BI Ke  HU Jingjie  ZHANG Can  ZHANG Quanqi  HU Xiaoli 《海洋学报(英文版)》2006,25(3):108-115

1IntroductionThe class bivalvia includes many well-knownmarine invertebrate species because they play impor-tant roles in aquaculture.Most cytogenetic studies ofbivalvia have focused primarily on chromosome num-ber and gross morphology(Insua et al.,1998).…  相似文献   

Phylogenetic relationship of Podocopida (Ostracoda:Podocopa) based on 18S ribosomal DNA sequences   总被引：1，自引：0，他引：1  

YU N  ZHAO Meiying  CHEN Liqiao  YANG Pin 《海洋学报(英文版)》2006,25(2):99-108

1 IntroductionOstracodaisoneofthelargestgroupsoftheCrus-tacea (M artin and Davis, 2001), and the num bersofliving speciesofostracodesconsistof7 500. OneofthefrustratingaspectsforOstracodastudyisunstabili-tyofthehigherclassification status (Schram, 1986),w…  相似文献