Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones   总被引：2，自引：0，他引：2  

DU Zongjun  ZHANG Wanyi  XIA Hongjie  L&#; Guoqiang  CHEN Guanjun 《海洋学报(英文版)》2010,29(2):62-69

A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea anemones. Samples of the sea anemones Anthopleura midori were collected from the coast of Weihai and bacteria were isolated from these samples. Additionally, high numbers of viable bacteria were obtained from the celom wall and surface of anemone, the community of cultivable bacteria was very diverse. As a result of this isolation, 60 strains were obtained, 56 of them were selected for identification and characterization by 16S rRNA gene sequence analysis and limited phenotypic testing. Among these isolates, 16 strains were phylogenetically related to members of the genus Pseudoalteromonas and neighboring taxa. Other isolates included members of the genera Colwellia, Vibrio, Acinetobacter, Pseudomonas, Endozoicomonas, Roseovarius, Paracoccus, Loktanella, Leisingera, Sulfitobacter, Bacillus, Staphylococcus, Plantibacter, Microbacterium, Micrococcus, Joostella, Psychroserpens, Cellulophaga, Krokinobacter, Polaribacter and Psychrobacter. Seven potential novel species were found. Among 60 strains, 17 of them can produce proteolytic exoenzyme, 20 can produce lipolytic exoenzyme. Strain NQ8 has strong antagonistic effects on some Vibrio strains. This study demonstrates that the culturable fraction of bacteria from the sea anemones Anthopleura midori is diverse and appears to possess much potential as a source for the discovery of novel bioactive materials.  相似文献   

小波变换在HT-7Tokamak磁流体动力学振荡动态频谱分析中的应用     

马天鹏  胡立群  陈开云 《海洋学报》2010,32(10):7209-7213

将Gauss复小波变换方法成功地应用于HT-7Tokamak磁流体动力学振荡动态频谱分析中.研究结果表明,这种方法具有较好的时间分辨率和空间分辨率,比较适用做动态频谱分析.对典型放电数据的分析结果表明, m=1模的振荡频率与等离子体压强梯度有着密切的关系.  相似文献   

渤海湾天津近岸表层沉积物中细菌丰度及其与环境因子的相关性研究   总被引：2，自引：0，他引：2  

肖慧  唐学玺  乔旭东  张喆  曲良 《中国海洋大学学报(自然科学版)》2010,40(6)

于2003年7月—2004年5月间,4个航次测定了渤海湾近岸表层沉积物中的可培养异养菌数、总菌数和活菌数,并分析了其与10项环境因子之间的相关性。结果表明,异养菌数变化范围为(1.1~470.0)×103CFU/g湿泥;总菌数和活菌数分别为(2.6~25.0)×107/g湿泥和(1.6~14.6)×107/g湿泥。异养菌数的季节分布为夏季最高,春季次之,冬季最低;站位间分布为最高值基本在沿岸或排污口附近的站位,最小值基本在远岸或远排污口的站位。沉积物中总菌数和活菌数最高值均出现在秋季,最低值出现在冬季,但次高值有所不同;各站点间的分布没有明显规律。此外,沉积物中仅异养菌数与总有机碳(Total organic carbon,TOC)和总氮(Total nitrogen,TN)之间有着显著的相关性,相关系数分别为0.568和0.598(P0.05);活菌数与铅(Pb)浓度显著负相关,相关系数为-0.751(P0.05)。  相似文献   

Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4     

MA Yuexin  LIU Pengliang  ZHANG Yongsheng  CAO Shanmao  LI Dantong  CHEN Wei 《海洋学报(英文版)》2010,29(1):69-78

The e?ect of the bacterial strain CI4 on the germination of spores from the green alga Ulva pertusa was assayed and it was found that the bacterial bioˉlm and cell-free supernatant strongly inhibited spore germination. In attempts to deˉne the chemical nature of the antifouling substance in the supernatant of CI4, the culture supernatants were tested for activity after heat treatment, enzy- matic treatments, size fractionation, and separation into aqueous and organic fractions. Results suggest that this bacterium produces an extracellular component with speciˉc activity toward algal spores that was heat-sensitive and between 3 and 10 kDa in molecular size. The exposure of the organic phase fraction to spores showed inhibitive e?ect on spore germination. Pronase and car- boxypeptidase y did not signiˉcantly a?ect the activity of inhibitory component, suggesting that the component was not a protein or a peptide. The bacterium CI4 was identiˉed as Pseudoalteromonas haloplanktis based on the phenotypic characters and 16S rRNA gene analysis.  相似文献   

蓝洞中的“外星”细菌群落     

罗洪斌 《海洋世界》2012,(5):51-53

蓝洞,很简单,就是蓝色的洞穴,会自己发出神秘的蓝光。早在古罗马时期就记载有人探险蓝洞,结论竟然是巫婆修练＂葵花宝典＂的秘密基地,这使得蓝洞变得愈发的引人入胜。  相似文献   

湖南省1：20万地物化重磁图形库建设的体会     

蔡让平  李元卓 《湖南地质》1999,18(1):61-63

本文概述了湖南省１：２０万地物化重磁图形库建设中取得的主要成果，从实践中摸索了一些图形处理的新方法，并指出了目前图形库建设中存在的问题及建议。  相似文献   

细菌干掉“深水地平线”泄露气体？     

潘峰 《海洋世界》2012,(2):60-63

墨西哥湾＂深水地平线＂漏油事件后,科学家大卫·瓦伦丁和他的同行于2011年早期发表了关于＂细菌的过量繁殖消耗掉了几乎所有泄露的甲烷气体＂的研究成果。随后,一些人对这位加州大学圣巴巴拉分校（UCSB）的地球化学家提出了质疑：泄漏出的甲烷气体是怎样消失的呢？在新一期的《美国国家科学院学报》中,瓦伦丁和加州大学圣巴巴拉分校的机械工程师伊戈尔以及其他合作者们报告了他们的新研究成果。他们采用一种新的计算机模型来论证墨西哥湾中水下地形、洋流和细菌在消耗泄露甲烷气体中所扮演的不同角色。  相似文献   

Heterotrophic bacteria of the Dapeng Bay in the South China Sea: community structure, abundance, and the relationships of culturablity with environmental factors   总被引：1，自引：0，他引：1  

JIANG Fajun  HU Zhangli  HU Chaoqun 《海洋学报(英文版)》2010,29(4):88-97

The community composition,abundance and culturablity of heterotrophic bacterioplankton in the Dapeng Bay of the South China Sea were investigated by morphological method,bacterial cultivation and biochemical methods over an annual cycle.Aeromonas and Pseudomonas were the predominant genera among the Gram-negative bacteria.The bacterioplankton abundance fluctuated from 2.07×10 8 to 2.44×10 9 cells/dm 3 ,and culturable bacteria abundance ranged between 0.1×10 6 and 16.07×10 6 CFU/dm 3 .The Culturablity of heterotrophic bacteria ranged between 0.01% and 1.31%.The relationship between bacterial culturablity and the environmental factors was also studied in different sites of the Dapeng Bay.Throughout the bay,a significantly positive relationship was evidenced between bacterial culturability and temperature,meanswhile bacterial culturability was significantly inversely related with salinity.The positive relationship was shown at the central bay while a negative relationship was observed at the bay mouth and there was no significant correlation at top bay between bacterial culturablity and chlorophyll a of sea water.A positive relationship was found between culturability and DIN all the bay,but DIP showed a significantly negative relationship with bacterial culturability only at the mouth of the bay.  相似文献   

青藏高原唐古拉哈日钦冰芯表层和深层可培养细菌特征   总被引：1，自引：1，他引：0  

王凤  王宁练  徐柏青  沈亮  顾政权  刘晓波 《冰川冻土》2019,41(4):968-976

对唐古拉哈日钦冰芯表层0.04~3.04 m及深层127.44~130.36 m中可培养细菌进行了对比研究。发现表层和深层可培养细菌数没有表现出显著差异性,表层冰芯中可培养细菌数为0~9.8×10³ CFU·mL^-1,略高于深层冰芯的0~8.4×10³ CFU·mL^-1。表层冰芯中微生物总数为10³~10⁶ cells·mL^-1,深层冰芯中微生物总数为10²~10³ cells·mL^-1。冰芯中可培养细菌属于Firmicutes（厚壁菌门）、Proteobacteria（变形菌门）、Actinobacteria（放线菌门）、Bacteroidetes（拟杆菌门）和Deinococcus-Thermus（异常球菌门） 5大类。但表层和深层可培养细菌属于不同的优势门,表层为Proteobacteria（38%）,深层为Firmicutes（42%）。表层和深层优势属均为Bacillus（芽孢杆菌属）,占比分别为18%和29%。已有研究表明哈日钦冰芯98.8 m达到了公元1000年的历史记录,因此对比千年尺度上可培养细菌数量及多样性的差异,能够为进一步发掘新基因,丰富微生物多样性,为了解可培养细菌的进化历史奠定基础。  相似文献   

1:5万重磁勘查在安徽宣城覆盖区地质找矿中的应用探索          下载免费PDF全文

陶龙  张莎莎  兰学毅  汤正江  安明  严加永  王云云  郭冬  叶林  洪大军 《中国地质》2019,46(4):894-905

安徽省宣城地区是长江中下游成矿带的重要组成部分,地表以第四系覆盖下（红层覆盖、推覆构造下）为主要特点。本文以最新获取的1：5万高精度重磁资料为基础,通过对研究区进行平面解译,获得了区内地质构造、控矿层位、侵入岩体的分布等平面属性;以研究区重磁二度半定量反演计算成果为基础,探索建立了全区准三维立体结构,掌握了区内主要控矿断裂、岩浆岩、矿源层等要素的几何空间展布规律及成矿有利部位等重要找矿空间属性;以研究区内已知典型矿床为切入点,总结了其地球物理场特征、成矿条件、控矿要素等成矿因素,探索构建了目标区地质-地球物理找矿模型,划分了成矿远景区,并圈定了重点找矿靶区。朱桥成矿远景区魏墩靶区钻探验证见矿实例表明,通过1：5万高精度重磁资料的处理与研究,进行位场平面—三维勘查、地质-地球物理找矿模型构建的方式发挥了地球物理找矿的优势,提高了找矿效果。本文研究成果可为中国东部覆盖区找矿勘查工作提供参考和指示。  相似文献