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A strain of thermophilic fungus Aspergillus fumigatus was cultured with K-bearing minerals to determine if microbe-mineral interactions enhance the release of mineralic potassium. Experiments were carried out in two settings, one with the mineral grains and the fungal cells in direct contact, and the other employing a membrane (pore size 0.22 μm) to separate the two. Measurements over a period of 30 days showed that, irrespective of the experimental setup, the concentration of free K in the culture was drastically higher than those in any of the control experiments where no living organism was present. Moreover, the occurrence of mineral-cell physical contact enhanced potassium release by an additional factor of 3 to 4 in comparison to the separation experiments. For contact experiments, Electron Probe Microanalysis revealed the formation of mycelium-mineral aggregates, and Atomic Force Microscopy imaging further indicated the possible ingestion of mineral particles by the fungus cells. Contrasting to what was observed and expected in control experiments, the potassium solubilization rate showed a positive dependence upon pH when fungi and minerals were mixed directly, and exhibited no correlations with solution acidity if cell-rock contact was restrained. These results appear to suggest that A. fumigatus promoted potassium release by means of at least three likely routes, one through the complexation of soluble organic ligands, another appealing to the immobile biopolymers such as the insoluble components of secretion, and the third related to the mechanical forces in association with the direct physical contact between cells and mineral particles. 相似文献
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Lian Gan Ni Kan Hu Kai Guo Dawei . Professor Senior Engineer Marine Design & Research Institute of China Shanghai
. Senior Engineer Marine Design & REsearch Institute of China Shanghai
. Engineer Marine Design & Research Institute of China Shanghai 《中国海洋工程》1992,(4)
This paper describes the study of a single-column structure used as well-head platform. In order to check the reliability of computation theory and programme, model tests have been carried out. The paper introduces the conclusion of tests and the dynamic properties of single-column platform are obtained. 相似文献
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对琉球群岛东部海区123个表层沉积物样品中浮游有孔虫因子分析结果表明,浮游有孔虫组合在溶跃面以上表现为Globigerinoides ruber-Globigerinata glutinata组合,溶跃面以下为Globorotalia inflata-Neoglobo-quadrina pachyderma (dex.)组合,局部受黑潮影响区域黑潮标志种Pulleniatina obliquiloculata含量相对丰富,为Neogloboquadrina dutertrei-P.obliquiloculata组合;表层沉积物中浮游有孔虫的丰度及组合分布特征对于碳酸盐溶跃面深度及碳酸盐补偿深度有一定的指示意义。另外,碳酸盐保存特征研究表明,该区域现代碳酸盐溶跃面深度约为3100 m,碳酸盐补偿深度约为4300 m。 相似文献
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本文介绍了海洋技术研究所质量管理处在维护质量体系运行方面的主要作法和经验体会,结合2000版ISO9201标准的新内容,提出适应新版标准的想法。 相似文献
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The model QQC_(2-2) plankton net closing gear is a device designed to control the stratifiedsampling of the plankton net by means of a messenger. The device in its fie1d use has met with98% success in 766 times of strtified sampling of plankton at 157 stations during separatesurveys of Fujian coast zone, the Taiwan Strait, and the central section of the South China Sea, 相似文献
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黄山高速公路穿行于皖南山区,公路建设过程中,汤口互通A匝道高边坡产生了大范围变形,严重威胁已有构筑物和施工安全。为保证边坡稳定,成立“应急指挥小组”,建立快速反应机制,并采用工程地质测绘、勘探、监测等手段,快速查明边坡结构特征,分析边坡变形机理和稳定性,提出应急治理方案。分析表明,该边坡为层间软弱夹层[JP2]发育的砂泥岩互层型顺向边坡,地质历史上边坡曾发生滑移—弯曲变形,公路施工时对弯曲隆起部位岩体进行开挖,引起坡体产生快速变形,在降雨、开挖等条件下,边坡产生整体失稳的可能性极大。在分析边坡变形机制基础上,应用变形控制理论,确定对边坡采取桩锚结合、综合排水的应急治理措施,并根据变形特点提出了应急治理施工步骤。应急治理措施实施后,边坡变形得到了有效控制,稳定性达到了高速公路的安全要求 相似文献
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Effect of Bacillus mucilaginosus on weathering of phosphorite and a preliminary analysis of bacterial proteins 总被引:3,自引:0,他引:3
The authors investigated the effect of Bacillus mucilaginosus on weathering of phosphorite. Analysis of different proteins was of significance in exploring the molecular biological mechanism in the bacterial weathering process. The concrete methods are described as follows: Mineral powder was put into liquid culture medium and B. mucilaginosus was incubated in the medium. The control (group) had no mineral powder in the medium. The treatments and controls were cultured simultaneously under the same condition. In a few days, the supernatant was filtrated, the main cations (Ca^2+, Mg^2+, Na^+, Mn^2+, Al^3+, Fe^3+, K^+) were measured by ICP-OES, and the contents of water soluble phosphorus (Pws) and silicon (Siws) were determined by colorimetry. The residual solid was weighed on the filter paper, followed by digestion with concentrated HNO3. The concentrations of the main cations and Pws, Siws in the digest liquid were measured by using the method mentioned above. After the supernatant was centrifuged, the precipitation was used to analyze the protein differences between the treatment groups and the control groups by 2-dimentional gel electrophoresis (2-DE). The experimental results showed that apatite and quartz were partially weathered, but kaolinite was dissolved completely. The population of bacteria increased when mineral powder was added in the liquid medium. Software analysis and comparison of the 2-DE pictures of bacterial proteins revealed 1134 visible protein spots in the treatment group, and 729 visible protein spots in the control group. To compare the bacterial protein expression contents of the treatment group with those of the control group, there were 496 different protein spots, including 214 protein spots which indicated that the protein contents increased, 75 protein spots were indicative of a decrease, and 207 proteins were newly synthesized. It is proposed that the increased bacterial contents may be related to some protein expression and activation after the 相似文献
10.
松散堆积体工程边坡变形机理分析及支护对策研究 总被引:1,自引:0,他引:1
合理选择开挖坡比、正确认识变形破坏机理是影响松散堆积体边坡稳定性和施工安全的前提, 本文研究了西南地区某松散堆积体工程边坡的结构特征, 根据地形条件确定了合理的开挖坡比, 采用二维有限元研究了开挖边坡的变形机理并根据模拟结果确定潜在滑动面, 在此基础上, 提出支护对策。研究结果表明, 边坡由厚度达70m 的坡洪积、泥石流块碎石堆积体组成, 斜坡下部缓中部稍陡, 开挖平台位于缓坡部位, 根据地形条件结合坡体结构特征确定边坡开挖坡比为1: 0. 75; 数值模拟结果表明, 边坡变形开挖面附近及坡顶拉应力和坡体下部最大剪应力控制, 坡顶部位将首先产生拉张裂隙, 开挖边坡内部产生从坡脚部位向上发展的剪切变形, 滑面一旦与坡顶拉裂缝贯通, 边坡将产生整体失稳; 边坡采用锚拉桩、锚索框架、锚杆框架、排水相结合的综合治理措施进行支护。 相似文献