Use of Sonication for Enhanced Sampling of Attached Microbes from Groundwater Systems     

Murray Close  Phil Abraham  Judith Webber  Eliza Cowey  Bronwyn Humphries  Graham Fenwick  Simon Howard  Kimberly Huynh  Travis Grace  Pierre-Yves Dupont  Louise Weaver 《Ground water》2020,58(6):901-912

The vast majority of microorganisms in aquifers live as biofilms on sediment surfaces, which presents significant challenges for sampling as only the suspended microbes will be sampled through normal pumping. The use of a down-well low frequency sonicator has been suggested as a method of detaching microbes from the biofilm and allowing rapid sampling of this community. We developed a portable, easy to use, low-frequency electric sonicator and evaluated its performance for a range of well depths (tested up to 42 m below ground level) and casing types. Three sonicators were characterized in laboratory experiments using a 1 m long tank filled with pea gravel. These included a commercially available pneumatic sonicator, a rotating flexible shaft sonicator, and the prototype electric sonicator. The electric sonicator detached between 56 and 74% of microbes grown on gravel-containing biobags at distances ranging between 2 and 50 cm from the sonicator. The field testing comprises of a total of 55 sampling events from 48 wells located in 4 regions throughout New Zealand. Pre- and post-sonication samples showed an average 33 times increase in bacterial counts. Microbial sequence data showed that the same classes are present in pre- and post-sonicated samples and only slight differences were seen in the proportions present. The sampling process was rapid and the significant increases in bacterial counts mean that microbial samples can be quickly obtained from wells, which permits more detailed analysis than previously possible.  相似文献   

Wetlands and low-gradient topography are associated with longer hydrologic transit times in Precambrian Shield headwater catchments     

Daniel Lane  Colin P.R. McCarter  Murray Richardson  Chris McConnell  Tim Field  Huaxia Yao  George Arhonditsis  Carl P.J. Mitchell 《水文研究》2020,34(3):598-614

The estimation of hydrologic transit times in a catchment provides insights into the integrated effects of water storage, mixing dynamics, and runoff generation processes. There has been limited effort to estimate transit times in southern boreal Precambrian Shield landscapes, which are characteristically heterogeneous with surface cover including till, thin soils, bedrock outcrops, and depressional wetland features that play contrasting hydrologic roles. This study presents approximately 3.5 years of precipitation and streamflow water isotope data and estimates mean transit times (MTTs) and the young water fraction (p_y) across six small catchments in the Muskoka-Haliburton region of south-central Ontario. The main objectives were to define a typical range of MTTs for headwater catchments in this region and to identify landscape variables that best explain differences in MTTs/p_y using airborne light detection and ranging and digital terrain analysis. Of the transit time distributions, the two parallel linear reservoir and gamma distributions best describe the hydrology of these catchments, particularly because of their ability to capture more extreme changes related to events such as snowmelt. The estimated MTTs, regardless of the modelling approach or distribution used, are positively associated with the percent wetland area and negatively with mean slope in the catchments. In this landscape, low-gradient features such as wetlands increase catchment scale water storage when antecedent conditions are dryer and decrease transit times when there is a moisture surplus, which plausibly explains the increases in MTTs and mean annual runoff from catchments with significant coverage of these landscape features.  相似文献   

North/South Hemispheric Periodicities in the >25 MeV${>}\,25\mbox{ MeV}$ Solar Proton Event Rate During the Rising and Peak Phases of Solar Cycle 24     

I. G. Richardson  T. T. von Rosenvinge  H. V. Cane 《Solar physics》2016,291(7):2117-2134

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Structure and evolution of mass transport deposits in the South Caspian Basin,Azerbaijan     

Steve E. J. Richardson  Richard J. Davies  Mark B. Allen  Simon F. Grant 《Basin Research》2011,23(6):702-719

The Quaternary to late Pliocene sedimentary succession along the margin of the South Caspian Basin contains numerous kilometre‐scale submarine slope failures, which were sourced along the basin slope and from the inclined flanks of contemporaneous anticlines. This study uses three‐dimensional (3D) seismic reflection data to visualise the internal structure of 27 mass transport deposits and catalogues the syndepositional structures contained within them. These are used to interpret emplacement processes occurring during submarine slope failure. The deposits consist of three linked structural domains: extensional, translational and compressive, each containing characteristic structures. Novel features are present within the mass transport deposits: (1) a diverging retrogression of the headwall scarp; (2) the absence of a conventional headwall scarp around growth stratal pinch outs; (3) restraining bends in the lateral margin; (4) a downslope increase in the throw of thrust faults. The results of this study shed light on the deformation that occurred during submarine slope failure, and highlight an important geological process in the evolution of the South Caspian Basin margin.  相似文献   

Shocks and sheaths in the heliosphere     

John D. Richardson 《Journal of Atmospheric and Solar》2011,73(11-12):1385-1389

This paper compares three kinds of shocks and sheaths; the bow shock and magnetosheaths of planets, interplanetary coronal mass ejection (ICME) shocks and sheaths, and the termination shock and heliosheath. We compare the energy balance across these shocks, fluctuations in the sheaths, asymmetries, and shock evolution. All three shocks and sheaths display asymmetries imposed by outside magnetic fields. Energy balance is often achieved through reflection of ions of the shocks, but in some cases no reflected ions are observed. The shocks may have similar small scale fluctuations which drive density changes in the sheaths.  相似文献   

Emerging coral diseases: a temperature‐driven process?          下载免费PDF全文

Aaron W. Miller  Laurie L. Richardson 《Marine Ecology》2015,36(3):278-291

In the last few decades there has been a surge in research focusing on coral disease. While climate change, specifically rising sea surface temperature, has been proposed as a major and growing driver of the emergence of marine diseases, to date a solid connection between disease epizootics and elevated sea surface temperature has not been established. However, a wealth of data now exists, compiled from many different perspectives, that may support such a connection. In this work we provide a comprehensive review targeting one coral disease, black band disease, that spans the infection process, pathobiology, and epizootiology, and links specific mechanisms of the disease process to increasing temperatures. This temperature‐driven pattern of infection can be expanded to include similar processes associated with other temperature‐related coral diseases. The conclusions presented here are based upon the results of many studies using a diverse suite of approaches that have been synthesized to argue that the emergence and continuing spread of black band disease is linked to warming sea surface temperatures. In summary, as global ocean temperatures increase seasonally and over decades, the environment shifts to become more favorable for the growth of potentially pathogenic microorganisms endemic to the immediate environment of the reef. The increase in the relative number of potential pathogens in the microbial community produces microenvironments conducive to the growth of other potential pathogens, leading to infection by a polymicrobial consortium. This consortium is easily perturbed by a (seasonal) temperature decrease, but remains associated with the coral host and can be reactivated with a subsequent seasonal increase in temperature, resulting in a cycle of temperature‐dependent disease emergence.  相似文献   

A nonlinear stability analysis of convection in a generalized incompressible fluid     

Lorna Richardson 《地球物理与天体物理流体动力学》2013,107(1-4):169-182

Abstract

We establish a nonlinear stability result for convection in a generalized incompressible fluid. Both numerical calculations and an asymptotic analysis are carried out. The linear and nonlinear results are shown to be very close in both cases, implying that the region of possible subcritical instabilities is very small.

During this work I was supported by a research studentship awarded by the Science and Engineering Council of the United Kingdom.  相似文献   

On a Fuller's Earth section at Combe Hay,near Bath     

L. Richardson 《Proceedings of the Geologists' Association. Geologists' Association》1910,21(8):425-428

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Ordinary Meeting,February 4th, 1870     

C.T. Richardson 《Proceedings of the Geologists' Association. Geologists' Association》1871,2(1):1-4

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Week-end field meeting in the Witney District: 25th and 26th May, 1935     

L. Richardson 《Proceedings of the Geologists' Association. Geologists' Association》1935,46(3):403-IN16

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