Ocean Science Journal - A carbonic anhydrase VII gene, encoding 277 amino acids, was identified in the intestinal tissue of pufferfish (Takifugu rubripes). The translated protein with an 833-bp... 相似文献
Ocean Science Journal - Investigations on marine N2 fixation have gained momentum since 1960s with eventual establishments of relevant methodologies to identify species involved and quantify the... 相似文献
Based on the three-dimensional digital image correlation (3D-DIC) technique, the stereovision system has been applied to the improved triaxial apparatus to obtain 3D full-field deformation of the specimen during triaxial testing. Through the calibration process, the 3D-DIC technique can obtain the accurate specimen’s spatial displacement deformation. Meanwhile, a subpixel edge detection algorithm has been combined with 3D-DIC technique to calculate the radial strain and the volume strain of the specimen directly. Furthermore, a series of consolidated drained and undrained triaxial tests were carried out on Hainan (China) sand specimens and measured by the conventional and the image measurement methods. Compared to the results measured by the conventional method, the image measurement technique can obtain the more experimental data, such as the 3D displacement field of the whole specimen, the local strain distribution, and so on. The measurement results also show the conventional method would be disturbed by the end constraints in triaxial tests so that the strength of the soil would be overestimated. Meanwhile, the middle of the specimen would be selected to calculate the stress–strain relationship without the influence of the end constraints in the proposed method. Based on the image measurement results, the proposed method has the potential to be used in geotechnical tests for exploring the soil’s progressive failure behaviors, inhomogeneous deformation and mechanical characteristics.
Morphogenesis of a population of the marine euplotid ciliate, Uronychia binucleata, which was found in Yellow Sea coastal waters next to a sewage outfall at a beach near Zhanqiao Pier, Qingdao, China, was investigated using protargol staining. The main pattern of morphogenesis is typical for the genus and can be summarized as follows: 1) the oral primordium in both proter and opisthe develops de novo in a subcortical pouch. In each daughter cell, the developing adoral zone of membranelles divides into two parts. The new membranelles formed in the proter's oral primordium will replace the leftmost five parental ones; six parental membranelles are retained by the proter; 2) the undulating membranes anlage is formed and develops independently from the oral primordium within the same subcortical pouch; 3) five primary front oventral-transverse cirral anlagen appear de novo on the cell surface; 4) the marginal cirral anlagen are formed de novo; 5) the leftmost frontal cirrus develops de novo on the cell surface; 6) two caudal cirri are formed at the posterior end of the rightmost anlage while the second primordium from the right gives rise to the third caudal cirrus. In contrast to its congeners, the anlage of the leftmost frontal cirrus is formed to the right of the undulating membranes anlage and before the formation of the latter. 相似文献
To address challenges in stochastic seismic analysis of nonlinear structures, this paper further develops a recently proposed Gaussian mixture–based equivalent linearization method (GM‐ELM). The GM‐ELM uses a Gaussian mixture distribution model to approximate the probabilistic distribution of a nonlinear system response. Using properties of the Gaussian mixture model, GM‐ELM can decompose the non‐Gaussian response of a nonlinear system into multiple Gaussian responses of linear single–degree of freedom oscillators. With the set of the equivalent linear systems identified by GM‐ELM, response statistics as crossing rate and first‐passage probability can be computed conveniently using theories of linear random vibration analysis. However, the original version of GM‐ELM may lead to an inaccurate estimate because of the heuristic parameters of the linear system introduced to supplement insufficient information. To overcome this limitation and define unique equivalent linear systems, this paper proposes a further developed version of GM‐ELM, which uses a mixture of bivariate Gaussian densities instead of univariate models. Moreover, to facilitate the use of elastic response spectra for estimating the mean peak responses of a nonlinear structure, a new response spectrum combination rule is proposed for GM‐ELM. Two numerical examples of hysteretic structural systems are presented in this paper to illustrate the application of the bivariate GM‐ELM to nonlinear stochastic seismic analysis. The analysis results obtained by the bivariate GM‐ELM are compared with those obtained by the univariate GM‐ELM, the conventional equivalent linearization method, the tail equivalent linearization method, and Monte Carlo simulation. The supporting source code and data are available for download at https://github.com/yisangri/GitHub‐bGM‐ELM‐code.git 相似文献
Solid matrix 3H reference materials are challenging to prepare given the volatile nature of 3H and are often unrepresentative of the range of 3H forms that may be encountered during routine analysis. As a result, few 3H reference materials are currently available, undermining verification of analytical techniques for environmental 3H measurement. To address this, an International Working Group on Organically‐Bound Tritium Analysis determined to produce a tritium natural matrix reference material (NMRM). The reference material comprises marine sediment blended with sewage sludge contaminated with 3H‐organic species arising from authorised discharges from a radiopharmaceutical manufacturing site. Previous studies have demonstrated that the 3H species have persisted in the environment over three decades providing valuable supporting data to underpin the characterisation of the NMRM. The preparation and characterisation of the NMRM are described along with the subsequent application of the reference material in an international intercomparison exercise involving nineteen laboratories from nine countries. A reference value of 168 ± 22 Bq kg?1 was derived from the data arising from the proficiency test. 相似文献
Geotechnical and Geological Engineering - A shale rock sample was scanned by micro-CT technology, tomographic images of the rock sample were obtained, and the three-dimensional structure was... 相似文献
Microbes live throughout the soil profile. Microbial communities in subsurface horizons are impacted by a saltwater–freshwater transition zone formed by seawater intrusion (SWI) in coastal regions. The main purpose of this study is to explore the changes in microbial communities within the soil profile because of SWI. The study characterizes the depth-dependent distributions of bacterial and archaeal communities through high-throughput sequencing of 16S rRNA gene amplicons by collecting surface soil and deep core samples at nine soil depths in Longkou City, China. The results showed that although microbial communities were considerably impacted by SWI in both horizontal and vertical domains, the extent of these effects was variable. The soil depth strongly influenced the microbial communities, and the microbial diversity and community structure were significantly different (p < 0.05) at various depths. Compared with SWI, soil depth was a greater influencing factor for microbial diversity and community structure. Furthermore, soil microbial community structure was closely related to the environmental conditions, among which the most significant environmental factors were soil depth, pH, organic carbon, and total nitrogen.
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