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建立了以经过遗传改造的发光细菌Acinetobacter sp. RecA为受试物种的环境污染物遗传毒性快速检测方法,该方法最快可在3h内得到毒性评价结果。采用该方法评价了环渤海排污口12份污水样品的遗传毒性。环渤海12个排污口的污水样品均表现出了不同水平的遗传毒性,并呈现出一定的分布特征。其中,高毒水质集中出现在山东半岛污水样品中,中毒水质集中出现在辽东半岛地区,而低毒水质主要集中于京津冀地区。综上,在环渤海的污水样品遗传毒性检测中,这种新型发光细菌法具有快速、灵敏、简便等优点,为以后近海环境的水质生物毒性检测提供了参考依据,具有应用于近海环境水质快速监测与评价的潜力。 相似文献
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万继康 《测绘与空间地理信息》2020,(1):72-75
针对研究城市热环境的过程中,利用归一化植被指数(NDVI)进行地表温度(LST)反演,再将LST和NDVI结合说明地物变迁与城市热环境的影响的现状,利用Landsat-8多时相遥感影像、高分辨率影像、公开GIS等多源数据,通过人工交互判读和量化统计分析,实现了2013—2017年北京建成区NDVI变化及其对地表热环境影响分析,再对分析结果进行差值拟合评价。对NDVI阈值分割按照大小为LC1、LC2、LC3、LC4,对LST分为高温区(TH)、常温区(TR)和低温区(TL)。结果表明,2013年至2017年:1)建成区的平均NDVI增加0.03,其中LC1增加1.0%,LC2减少11.6%,LC3区域减少1.7%,LC4区域增加12.3%。2)建成区平均LST增加2.55 K,TH百分比增加0.6%;TR百分比减少1.1%,TL百分比增加0.5%。3)NDVI相对增加,地表温度相对下降以及NDVI相对减少,地表温度相对上升占60%,NDVI相对下降,地表温度相对下降以及NDVI相对增加,地表温度相对上升的占40%。 相似文献
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本实验以64d为限,设A1(34%),A2(38%),A3(42%)三个蛋白梯度水平和B1(5%),B2(8%),B3(11%)三个脂肪梯度水平,采用正交试验方法研究了饲料中不同脂肪及蛋白质水平对日本沼虾(Macrobrachium nipponensis)[平均体重(0.69±0.23)g]生长性能、体成分和消化酶活力的影响。结果表明,脂肪和蛋白质水平对日本沼虾成活率影响不显著(P0.05),脂肪水平对32d相对增重率和特定生长率影响显著(P0.05)。B3组的特定生长率和相对增重率均显著低于B1和B2组(P0.05)。全虾和肌肉的脂肪含量显著地受饲料脂肪水平的影响(P0.05)。饲料中蛋白质水平对全虾的干物质、蛋白质和灰分含量,以及肌肉中脂肪、蛋白质含量影响显著(P0.05)。饲料脂肪水平对肝胰脏中脂肪酶活力影响显著(P0.05),蛋白水平对肝胰脏中脂肪酶、蛋白酶和淀粉酶活力影响不显著(P0.05)。本文认为日本沼虾饲料脂肪水平应以5%—8%较为合适,蛋白质水平应在38%左右较为合适。 相似文献
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Replacement of Dietary Fish Oil with Vegetable Oils Improves the Growth and Flesh Quality of Large Yellow Croaker （Larmichthys crocea） 总被引：2，自引：1，他引：1
DUAN Qingyuan MAI Kangsen SHENTU Jikang AI Qinghui ZHONG Huiying JIANG Yujian ZHANG Lu ZHANG Chunxiao GUO Sitong 《中国海洋大学学报(英文版)》2014,13(3):445-452
We investigated the effect of the replacement of dietary fish oil with vegetable oils on the growth and flesh quality of large yellow croaker(Larmichthys crocea). The basal diet(FO) was formulated to contain 66.5% fish meal and 6.4% menhaden fish oil; whereas the other 3 experimental diets were formulated by replacing the fish oil with 50% soybean oil(SO50), 100% soybean oil(SO100) and 100% palm oil(PO100), respectively. The 4 diets were randomly assigned to 4 floating sea cages(3.0 m × 3.0 m × 3.0 m), and each was stocked with 250 fish individuals with an initial average weight of 245.29 g ± 7.45 g. The fish were fed to apparent satiation twice a day at 5:00 and 17:00, respectively, for 12 weeks. Experimental analysis showed that the specific growth rate of fish fed SO50 or PO100 were significantly higher than that of fish fed FO or SO100(P0.05), and crude lipid contents of ventral muscle and viscera were significantly lower in fish fed FO than in those fed the other 3 diets(P0.05). No significant differences in condition factor, viscerosomatic index, hepatosomatic index, gutted yield and colorimetric values of fish among the dietary treatments were observed(P0.05). Compared to FO diet, SO50, SO100 and PO100 diets led to substantial decreases in the liquid loss and water loss from fresh fillets(1 d, 4℃)(P0.05). Similarly, thiobarbituric acid reactive substance(TBARS) values of fillets under different storage conditions(1 d, 4℃; 7 d, 4℃; 4 weeks,-20℃; 8 weeks,-20℃) decreased significantly after partial or complete replacement of fish oil with vegetable oils. These findings indicated that the growth performance and selected flesh quality properties(liquid holding capacity and TBARS value) of large yellow croaker were substantially improved by replacing dietary fish oil with vegetable oils. 相似文献
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水生动物往往会通过调整自身生理生化特征而形成各自固有的越冬生存适应对策,因此,深入探究越冬对具有冬眠习性的目标水产养殖动物机体生理状况的影响过程与机制,对指导其安全养殖具重要参考价值。以池塘专养模式下2+龄中华鳖(Trionyx sinensis)同生群个体为研究对象,从机体形态、血清生化、脏器相关功能酶活力和肌肉与裙边物性等四个维度开展了中华鳖越冬前后生理生化特征的差异研究,以进一步了解越冬对中华鳖生理生化的影响特征。结果表明:(1)越冬期间实验中华鳖总体上呈现较明显的生长态势,所测30项体尺性状和12项质量性状测定值间的差异相似性分别为46.7%和8.3%;(2)所测16项血清生化指标中,越冬后显著降低的为TG等5项(P<0.05),显著增加的为TP等4项(P<0.05),无显著差异的为GLB等7项(P>0.05);(3)所测8项脏器酶学指标中,越冬后酶活力显著下降的为肝脏CAT、POD和脂肪酶(P<0.05),显著升高的为胃蛋白酶等相关酶指标(P<0.05);(4)所测7项物性指标中,越冬后裙边弹性和咀嚼性均显著增加(P<0.05),其余为下降或无显著差异(P>0.05)。表明越冬为引起肝脏功能发生正常切换的重要时期,但不会引起心肝功能产生实质性改变。研究结果可为中华鳖越冬生理生态学研究和池养中华鳖越冬管理技术体系构建提供基础资料。 相似文献
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在三疣梭子蟹(Portunus trituberculatus)的单体筐养养殖系统中, 试验了生态掩体(砂盒)中不同的砂粒粒径大小以及砂层厚度对梭子蟹幼蟹摄食行为与生长特性的影响。砂粒粒径设三个水平,分别为: >2mm、<0.2mm 以及混合砂; 砂层厚度有0cm、2cm、5cm、8cm 四个水平。试验共进行6天, 结果表明: 砂粒粒径及砂层厚度对梭子蟹幼蟹的摄食与生长都有明显的影响。从砂粒粒径看, 幼蟹在细砂(SPS)中挖洞休息, 蟹体与砂面呈30—45°角, 仅露眼睛及触角在外。一天内有3—6 次进食,总进食时间为(142.7±22.52)min, 在摄食次数、总进食时间、平均摄食量[(0.2427±0.0137) g/gBW]、以及蜕皮及成活率都远高于其它粒径组, 该组中幼蟹的体重增长最快, 增加了(0.814±0.113)g, 增长率为91.5%±3.43%; 而该组的饵料系数(FCR)最低为1.17±0.11。因此, 筐养养殖系统砂掩体的砂粒粒径最好为0.2 mm 以下。砂层厚度也有类似结果, 5 cm 以上厚度养殖效果最佳。平均摄食量为(0.2087±0.0046) g/gBW, 该厚度下, 幼蟹无死亡、100%蜕皮, 体重也增加最快, 增加了(0.791±0.121)g, 增长率为88.9%±3.74%, 饵料系数(FCR)达到1.37±0.23, 表明筐养系统掩体中砂层厚度要在5 cm 以上。三种保护性酶类(SOD, POD, CAT)活性随着砂粒粒径变小和砂层厚度增加而降低, 而消化酶类(淀粉酶、蛋白酶、脂酶)活性则表现与保护性酶类相反特性。从两类酶的活性变化也能证实, 在优选条件下(细砂、厚度>5 cm), 幼蟹所受的胁迫在降低。 相似文献
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Effect of salinity on survival,feeding behavior and growth of juvenile swimming crab P ortunus trituberculatus was investigated under 5 salinity levels of 5,10,20,30 and 40. The results show that the crab juveniles fed 2 or 3 times at the salinity 20 and 30,each lasted for about 25 minutes,for a total feeding time of 73.2±22.65 minutes per day. At these salinities,there were significantly higher in the frequency of feeding and in total feeding time than those at lower salinities of 5 and 10. All crab juveniles moulted when reared at a salinity of 20 during the 5 days duration of the experiment,which is significantly higher than those at other salinities. All juveniles survived at salinity 20,and the survivorship was not significantly different from that at 30,but was signif icantly higher than those at other salinities. The crab juveniles reared at a salinity of 20 had the highest value of food ration of 0.190 8±0.011 3 g/g BW,average body weight gain of 0.796±0.128 g,gain rate of 87%–96%,and food conversion ratio of 1.20±0.09. There was no significant difference in the values found between 20 and 30 but these values were significantly lower than that at the other salinities( P 0.05). Highest activities of digestive enzymes(Amylase,Protease,Lipase) and lowest activities of protective enzymes(SOD,PO,CAT) were also obtained on crab juveniles reared at salinity of 20. 相似文献