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新鲜鱼油中添加05、0、2505、00 mg/kg 4种不同浓度的维生素E醋酸酯,在实验的第6、14、28、36和68天测定鱼油中的脂肪酸含量。结果表明:在500 mg/kg添加量内,维生素E醋酸酯对鱼油中脂肪酸含量没有显著性影响(P>0.05)。在实验期间,EPA(C20:5)、DHA(C22:6)、亚油酸(C18:2)含量明显下降,ARA(C20:4)、亚麻酸(C18:3)先升后降,棕榈酸(C16:1)逐步上升;C14含量逐渐增加,C17则先降后升,C16和C18在实验后期有较大提高。维生素E醋酸酯对鱼油中不饱和脂肪酸效用系数大小依次为:ARA>EPA>DHA>C18:3>0>C16:1>C18:2>C18:1,对ARA抗氧化效用最大,效用系数达到10.193%,C20:5为0.490%,C22:6为0.364%;维生素E醋酸酯对饱和脂肪酸效用系数大小依次为:C14>C17>0>C18>C16。 相似文献
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1 INTRODUCTION Active oxygen free radicals can damage cell structure, even kill the cells, causing aging and cer- tain serious diseases (Bors et al., 1989). Usually, the production and scavenging of active oxygen free radicals are in balance in healthy hu… 相似文献
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该文分别选用不同浓度的海螺酶 ;葡萄糖、蔗糖、甘露醇、山梨醇、氯化钠 5种渗透剂 ;维生素 C和甘露醇 2种抗氧化剂 ;对条斑紫菜酶解单细胞的成活率进行了研究。实验结果表明 :用浓度为 10 %的海螺酶酶解条斑紫菜 ,成活率最高 ;选用 2 mol/ L葡萄糖作渗透剂 ,效果最好 ,成活率达85.2 % ;加入抗氧化剂对细胞成活率没有影响 相似文献
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Stress Responses to Rapid Temperature Changes of the Juvenile Sea Cucumber （Apostichopusjaponicus Selenka） 总被引：2，自引：0，他引：2
DONG Yunwei JI Tingting DONG Shuanglin 《中国海洋大学学报(英文版)》2007,6(3):275-280
Activities of hexokinase （HK）, pyruvate kinase （PK）, superoxide dismutase （SOD） and catalase （CAT） and Hsp70 level were measured to evaluate the response of the commercially important sea cucumber （Apostichopus japonicus Selenka） to rapid temperature changes in laboratory. Animals were subjected to a higher temperature （from 10 to 20℃） （Tinc treatment） or to a lower temperature （from 20 to 10℃） （Tddec treatment） for 72 h. At 1, 3, 12, 24, 72 h of exposure, animals were removed and prepared for further analysis. Results showed that the effect of acute temperature changes on enzyme activities was significant. In Tinc treatment, activities of SOD and CAT increased immediately. The significant enhancement of SOD and CAT activities suggested that oxidative stress increases significantly when ambient temperature increasing from 10 to 20℃. The up-regulation of Flsp70 in Tinc and Tdec treatments indicated that Hsp70 was a bioindicator of thermal stress in the sea cucumber, and the expression pattern depended on the thermal treatment. 相似文献
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δ-维生素E和迷迭香在鲱鱼油中的抗氧化效果 总被引：6，自引：0，他引：6
在鲱鱼油中分别加入抗氧化剂δ-维生素 E,迷迭香及其混合物 ( KEMIN- Europe,含有 δ-维生素 E和迷迭香 )。在氧化诱导器 ( Metrohm Rancimat,80℃ )中测定每一抗氧化剂的诱导时间 ,结果表明 ,迷迭香比 δ-维生素 E稳定鱼油的性能好。混合天然的抗氧化剂较单一天然抗氧化剂效率要高 ,迷迭香和δ-维生素 E彼此具有协同效应。 相似文献
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光照、变性剂和pH对钝顶螺旋藻(Spirulina platensis)别藻蓝蛋白(APC)抗氧化活性的影响 总被引：2，自引：1，他引：2
采用不同光照条件、不同浓度的十二烷基硫酸钠(SDS)和脲，以及不同pH等条件处理钝顶螺旋藻别藻蓝蛋白(APC)，检测其光谱变化、生成及清除自由基能力的变化，对纯化的钝顶螺旋藻别藻蓝蛋白(APC)在不同条件下的抗氧化活性进行了研究。结果表明，光照下，APC具有生成自由基的能力；黑暗中，APC却表现为清除自由基。SDS是一种很强的变性剂，1mmol／L的SDS即可以使APC完全变性，能量传递功能丧失，光照下，APC生成自由基的能力丧失，自由基清除能力明显增强。1．6mol／L的脲作用后，只使APC部分变性，导致APC能量传递效率降低，光照下，表现为生成自由基的能力下降。随着脲浓度的升高(3．2mol／L、6．4mol／L)，APC的结构逐渐变化，能量传递功能逐渐丧失，表现为生成自由基的能力逐渐下降，清除自由基的功能逐渐增强。APC具有较宽的pH稳定性，在pH为7—10的范围内非常稳定；当pH为11时，APC的结构已经发生变化。在日光灯下，pH为7时，APC具有生成自由基的能力；pH为8时，APC的荧光光谱虽然没有发生变化，但它们表现为清除自由基的能力。随着pH的增加，自由基清除能力也增强。因此，APC具有产生和清除自由基的双重功能，光照是调控自由基清除与产生的关键因素，并且只有APC具有能量吸收和传递功能时，才具有产生自由基的能力。脱辅基蛋白变性后，清除自由基的能力加强。 相似文献
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Healthy sporophytes of two gametophyte mutants of Laminaria japonica with different heat resistances: kelp 901 (901, with comparatively stronger heat-resistance) and Rongcheng No.1 (RC, sensi- tive to heat stress), were respectively collected during October to December 2002 from Yantai and Rong- cheng Sea Farm in the Shandong Peninsula of China. The contents of some biochemical materials and antioxidant capacity were analyzed under controlled laboratory conditions to identify if there is any rela- tion between the overall antioxidant capacity and the heat-resistance in L. japonica and to understand possible mechanism of heat-resistance. Results show that: (1) the overall antioxidant capacity in healthy sporophyte of 901, such as vitamin E, polyphenol, and ascorbic acid contents and the enzymatic activity of SOD, POD, CAT, Gpx, PPO, and PAL, were not always higher than that of RC under controlled labo- ratory conditions, and no significance (P>0.05) was shown in total antioxidant capacity (T-AOC) in 901 and RC. Result suggested that the difference in antioxidant capacity was not a decisive factor for different heat-resistances in L. japonica; (2) the simultaneous assay on isozymes was carried out using vertical polyacrylamide gel electrophoresis (PAGE). Considerable differences in peroxide (PRX), malate dehy- drogenase (MDH), malic enzyme (ME), polyphenol oxidase (PPO) and glutamate dehydrogenase (GDH) were obtained in 901 and RC from either the band number, relative mobility (Rf), or staining intensity, and ME could be used as an indicator to distinguish healthy sporophyte of 901 and RC under controlled labo- ratory conditions. 相似文献