Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
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The cultivation and development of modern metropolitan areas with the aim of establishing new regional centers with competitive edge is a key objective for the new-type urbanization directions in China. The construction of the Lhasa Metropolitan Area is of great significance for the promotion of the South Asia Channel, the ‘Belt and Road' initiative, the Bangladesh-China-India-Myanmar Economic Corridor, the Himalaya Economic Cooperation Zone, and for rapid development and long-term stability of the Qinghai-Tibet Plateau. This paper examines the scope of the Lhasa Metropolitan Area including Chengguanqu(Chengguan District), Doilungdeqen, Dagze, Lhunzhub, Damxung, Nyemo, Quxu, Maizhokunggar, Samzhubze Qu(Samzhubze District), Gyangze, Rinbung, Bainang, Nedong, Gonggar, and Zhanang using a spatial field energy model that combines nodality and accessibility indices and considers multiple indicators including traffic flow between cities. By combining factors such as the natural background, population agglomeration, the social economy, infrastructure construction, and the urban spatial structure of the Lhasa Metropolitan Area, it is proposed to build a bow-and-arrow-shaped urban system with ‘one core, two centers, one axis, and two wings' along the valleys and the transportation trunk lines of the area. The study advocates the construction of a pure land industrial system comprising a green cultural and tourism-oriented plateau. 相似文献
大气细颗粒物PM2.5污染引起的雾霾天气既与本地污染物排放密切有关,也受局地特殊的风场影响.本文以武汉城市区域为研究对象,分别研究了长江沿岸的江陆风环流、东湖沿岸湖陆风环流的形成与转化特征,发现江风、湖风开始时间均为07：00-08：00,一年中最大风速均能达到2 m/s左右,而春夏季江风的持续时间高于秋冬季,夏季湖风的持续时间高于春季.同时发现区域附近温度和相对湿度之间有明显的相关性,湿度变化趋势大体上跟温度变化趋势相反,且温度对相对湿度的影响存在一定的滞后性,延迟时间大约为1 h. 相似文献
