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21.
Akira Shibata Kouichi Ohwada Masakazu Tsuchiya Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):91-97
Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate
organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted
for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present
study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater
cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size,
0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and
correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG
to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m,
which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell−1 obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29)
and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest
that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas
this fraction was reduced in the middle layer. 相似文献
22.
讨论了不同温度的有机溶剂分级提取对鳀鱼鱼油得率、脂肪酸组成及EPA、DHA含量变化的影响。经15℃干法分级提取的液态鳀鱼鱼油,在设定的4种温度下,液态鳀鱼鱼油得率均随溶剂量的增加而提高;在相同溶剂量的情况下,随着分级提取温度的降低,液态鳀鱼鱼油得率呈下降的趋势。有机溶剂分级提取对14碳短碳链的饱和与不饱和脂肪酸及16碳、18碳的饱和脂肪酸的脱除效果是明显的,但对脱除20碳、22碳的长碳链饱和脂肪酸的效果不明显。在4种试验温度下,EPA和DHA的总量分别为29.88%、31.74%、35.27%和41.80%,高出美国阿拉斯佳深海鱼油对应总量(28.76%)的1.12%~13.04%。 相似文献
23.
阐述把维生素B12生产菌添加到培养水中培养褶皱臂尾轮虫BrachionusPlicatilis的买验。共18株细菌分离于轮虫培养池,其中,有一株产维生素B12的假单胞杆菌TP4对轮虫的生长繁殖有明显的促进作用。把TP4菌株培养后,加入到2L的烧杯和500L的水槽中培养泰国S型轮虫时.在9d(天)和6d(天)中,轮虫密度从124~139和242~288个体/ml增殖到4,417~5,540和1,017~1,254个体/ml,分别比对照组增加了4~6及2~3倍。 相似文献
24.
pH对眼点拟微绿球藻的生长、总脂含量及脂肪酸组成的影响 总被引:9,自引:5,他引:9
pH对眼点拟微绿球藻(Namnocholoropsis oculata)的生长、总脂含量及脂肪酸组成有明显影响。研究表明,眼点拟微绿球藻的生长对pH有较强的适应性,在pH6.2~9.8的范围内均能较好的生长,但在pH5.5的条件下生长受到较强的抑制,在不同pH条件下,在同一细胞周期内总脂含量及脂肪酸组成均有相同的变化规律,即总脂含量在对数初期略有下降然后逐渐上升,在稳定期达到最大值。16:0,18:1n9的含量在稳定期显著升高,而EPA的含量在稳定期明显下降,在对数初期,藻细胞在pH6.8的条件下含有较高的EPA含量,而在pH9.8的条件下,藻细胞的EPA含量明显降低。 相似文献
25.
Young-Ok Kim Eun Jin Yang Jung-Hoon Kang Kyoungsoon Shin Man Chang Cheol Soo Myung 《Ocean Science Journal》2007,42(1):9-17
The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and
heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake
Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semienclosed, inner
area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. 0.4 mg L1) and high concentrations of inorganic nutrients (nitrogenous nutrients >36 μM, phosphate <4 μM) in the bottom layer. Higher
densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while
microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed
into more than 106 cells L1 at the surface layer of the inner area, while its abundance was much lower (103-104 cells L1) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p <
0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to theT. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing
of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates. 相似文献
26.
2002年2月—2004年11月对胶州湾异养细菌和大肠菌群进行长期调查发现,胶州湾异养细菌丰度在105—106cells/ml之间,其中河口区域和近岸区域数量较高,其它区域相对较低。从季节上来看,夏季、秋季细菌丰度较高(分别为7.4×105、7.2×105cells/ml),冬季和春季丰度较低(分别为4.1×105cells/ml、5.9×105cells/ml),且在一年内呈现出一定的规律性波动。从季节尺度上看异养细菌丰度与温度呈现很好的正相关性(R=0.54)。对大肠菌群的研究表明,河口区域的大肠菌群数量显著高于其它区域,并且河口区域大肠菌群常年处于严重超标状态(最高为1.1×106cells/L,超过国家前三类水质标准的100倍)。根据大肠菌群的数量状况可以将胶州湾分为三个区域,即严重污染区、中度污染区和轻度污染区。该区域划分与通过营养盐对胶州湾区域的划分(富营养区、中度营养区、贫营养区)结果一致。 相似文献
27.
用扁藻、酵母、“鱼油 酵母”强化和“不强化”4种方式处理的卤虫无节幼体投喂黑斑口虾蛄各期幼体,比较不同强化方法对黑斑口虾蛄幼体成活率、变态率、生长速度及总脂与脂肪酸组成的影响。结果表明,3种方式强化12h后的卤虫无节幼体与对照组相比,总脂含量都有不同程度的增加。卤虫无节幼体的脂肪酸组成与强化方式密切相关,其中“鱼油 酵母”强化的卤虫无节幼体20∶5n-3(EPA)和22∶6n-3(DHA)占总脂肪酸的比例最高,分别为5·74%和4·84%。投喂强化后富含EPA和DHA的卤虫无节幼体,可增加黑斑口虾蛄幼体体内脂肪酸尤其是EPA和DHA等不饱和脂肪酸的含量,从而提高其幼体的成活率、变态率与发育速度。 相似文献
28.
南海中部和冲绳海槽沉积物中的氨基酸物质 总被引:2,自引:0,他引:2
本文通过对南海中部和冲绳海槽三个深海沉积物柱状样腐植物质水解氨基酸的研究,试图阐述南海和冲绳海槽沉积物的演化与沉积环境。 相似文献
29.
Microbial Biomass Dynamics Along a Trophic Gradient at the Atlantic Barrier Reef off Belize (Central America) 总被引:2,自引:0,他引:2
Abstract. Recent findings indicate that heterotrophic bacteria and not phytoplankton are the most numerous biomass components even in the euphotic zone of oligotrophic, open oceans. In this study it was hypothesized that the microbial biomass components change within a few hundred meters as oligotrophic water flows across the reef and becomes enriched with nutrients. Along a trophic gradient, four stations at the Atlantic Barrier Reef off Belize (Central America) were sampled for microbial biomass components. Phytoplankton biomass (measured as chlorophyll a) ranged from the most oligotrophic station (St. 1) to the most eutrophic station (St. 4) from 6.9–415.5 μg CI"' (assuming a C:chl a ratio of 30): heterotrophic bacterial biomass increased 4-fold (from 10.1–46.4μg C 1-1 ), heterotrophic nanoflagellate (HNAN) biomass increased from 4.6-19ug C 1-1 , and cyanobacteria from 0.9-4.5 μg C-1-1 . Production estimates derived from seawater cultures revealed a 5-fold increase in bacterial production from the oligotrophic station (3.7 ug C 1-1 d-1 ) to the eutrophic St. 4 (17.8ug C-1-d1-1 )- Cyanobacterial production rose from 1.1–3.5ug C-1–d-1 and HNAN production from 0.65-1.13 μg C-1-1 -d-1 . While cyanobacteria contributed between 13 and 20% to the autotrophic plankton component in the oligotrophic waters, their contribution dropped to about 1 % at the eutrophic stations. 相似文献
30.