Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome   总被引：1，自引：0，他引：1  

闫秀英  孙修勤 《中国海洋湖沼学报》2009,27(2):337-341

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)  相似文献   

科学家发现蜥蜴的毛发基因     

《海洋世界》2008,(12):9-9

长期以来，科学家都认为毛发最初出现在哺乳动物中。毛发可为哺乳动物提供隔离和保护，一直被认为是一个生物进化上的创新。一项新研究表明，毛发的起源可以追溯到3亿年前古生代的爬行类。奥地利维也纳大学的科学家们发现，绿色变色蜥蜴的基因组中有6个编码角蛋白的基因（在哺乳动物中角蛋白与毛发生成密切相关），  相似文献   

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency     

Aibin Zhan  Zhenmin Bao  Xiaoli Hu  Wei Lu  Jingjie Hu 《中国海洋大学学报(英文版)》2009,8(2):161-165

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.  相似文献   

棘皮动物线粒体基因组的基因重排、分子标记及系统发生分析     

申欣  田美  孟学平  程汉良  阎斌伦 《海洋学报》2013,35(5):137-146

棘皮动物(echinoderms)是海洋生境中所特有的无脊椎动物重要类群,本文全面比较分析了棘皮动物29个物种的线粒体全基因组。线粒体基因组主编码基因的分析结果显示,海胆纲Echinoidea和海参纲Holothuroidea物种的基因排列完全相同;海星纲Asteroidea物种之间的基因排列也完全相同,然而与海胆纲、海参纲相比,存在一个长片段的倒位。海百合纲Crinoidea的栉羽星Phanogenia gracilis和花形羽枝Florometra serratissima主编码基因的基因排列完全相同,地中海海羊齿和海百合Neogymnocrinus richeri与此相比,均存在一个蛋白质编码基因(nad4L)的易位。蛇尾纲Ophiuroidea真蛇尾目Ophiurida的3个科(阳遂足科Amphiuridae、辐蛇尾科Ophiactidae和栉蛇尾科Ophiocomidae)主编码基因的基因排列完全相同,而同属于真蛇尾目,另外一个科(真蛇尾科Ophiuridae)的白色真蛇尾Ophiura albida和灰色真蛇尾Ophiura lutkeni,与同目的前3个科相比,存在3个蛋白质编码基因(nad1、nad2和cob)的倒位。蛇尾纲蔓蛇尾目Euryalida的海盘Astrospartus mediterraneus,与真蛇尾目5个线粒体基因组相比,存在主编码基因的重排。棘皮动物线粒体单基因的变异位点特征显示,nad5、nad4和nad2基因是理想的分子标记基因。基于29个线粒体基因组的氨基酸序列,通过两种方法(邻接法和最大似然法)所构建系统发生树的拓扑结构完全一致。支持其下分的5个纲(蛇尾纲、海参纲、海胆纲、海星纲和海百合纲)均为单系群。线粒体基因组的数据支持棘皮动物动物在纲层次的亲缘关系为:(((海胆纲+海星纲)+海参纲)+蛇尾纲)+海百合纲,海百合纲作为棘皮动物中最为古老的类群,位于系统发生树的根部。基于线粒体基因组构建的系统发生树,支持所有的科均为单系群;综合系统发生树及主编码基因的基因重排分析,均支持真蛇尾目并非单系发生,真蛇尾目的有效性还值得今后深入研究。  相似文献   

基于全基因组重测序技术的浙江近岸海域耐盐金黄色葡萄球菌(Staphylococcus aureus)耐药机制解析<     

张旨轩  王子言  王泽  刘岩  刘松怡  钱鹏宇  叶欢  韩姣姣  周君  苏秀榕 《海洋与湖沼》2022,53(2):394-404

金黄色葡萄球菌(Staphylococcus aureus)是近岸海域海水中的主要病原菌,严重威胁接触者的安全。抗生素处理是治疗金黄色葡萄球菌感染的重要手段,其耐药性的发生受到了高度重视。采用全基因组重测序与KEGG富集分析结合的方法,对红霉素(erythromycin)、氯霉素(chloramphenicol)和万古霉素(vancomycin)处理后的耐盐金黄色葡萄球菌(S.aureus ZS01)和不耐盐金黄色葡萄球菌(S.aureus 502A)进行耐药机制研究。结果表明,S.aureus 502A经抗生素处理后发生突变的程度大于S.aureus ZS01,二者在经过氯霉素处理发生了更大程度的突变。红霉素、氯霉素和万古霉素处理主要影响了金黄色葡萄球菌的致病能力;红霉素和氯霉素可能通过影响金黄色葡萄球菌脂类的代谢引起其耐药性的变化。除此之外,三种抗生素处理均出现了较多TIGR01741家族蛋白和假设蛋白基因的突变,推测与菌株的耐药性和致病性相关。耐盐金黄色葡萄球菌可通过外排系统作用产生红霉素耐药性,不耐盐菌株因细胞壁成分相关基因的突变提高了对万古霉素的耐受性。研究结果可为耐盐和不耐盐金黄色葡萄球菌的耐药机制及抗生素对金黄色葡萄球菌致病性影响的研究提供基础数据。  相似文献   

动物型溶菌酶研究新进展   总被引：3，自引：0，他引：3  

张士璀  许娜 《中国海洋大学学报(自然科学版)》2014,44(6):46-51

溶菌酶可以分解连接N-乙酰胞壁酸(NAM)和N-乙酰氨基葡萄糖(NAG)残基之间的β-1,4糖苷键。g型、c型和i型溶菌酶是3种动物型溶菌酶。近年来,越来越多的报道表明这3种类型的溶菌酶在动物界广泛存在,g型和c型溶菌酶主要存在于脊椎动物中,i型溶菌酶仅存在于无脊椎动物中,部分物种中同时存在2种动物型溶菌酶。本文简要概述了溶菌酶在动物各物种中的存在情况和组织分布情况、基因组结构与系统进化以及溶菌酶抑制剂的研究新进展。  相似文献   

人工诱导栉孔扇贝雌核发育胚胎的SNP标记分析     

陈亮  赵柏淞  李玲  商文聪  胡晓丽  包振民 《中国海洋大学学报(自然科学版)》2014,44(2)

利用紫外线照射灭活的同源精子,激活栉孔扇贝(Chlamys farreri)卵子分裂,获得雌核发育胚胎,流式细胞仪检测胚胎细胞平均单倍体率为82.5%。对获得的胚胎进行全基因组扩增,获得足量的基因分型模板,利用高分辨率溶解曲线(HRM)技术对其进行96个单核苷酸多态性(SNP)位点分型。结果显示,诱导雌核发育胚胎的位点检出率为96.27%,其中99.06%的位点分型结果与相应雌性亲本相符;单个胚胎中只表现一种等位形式的位点占91.67%~96.88%,平均为94.81%,较雌性亲本的纯合位点比例(64.25%)明显提高。本研究利用灭活同源精子诱导获得栉孔扇贝雌核发育胚胎,并利用单个胚胎的全基因组扩增产物进行基因位点分析,为扇贝人工诱导雌核发育个体的早期多基因位点检测和评价提供参考。  相似文献   

褐藻纲海带目Laminaria属和Saccharina属基于三个分子标记的系统发育分析     

CHI Shan  QIAN Hao  LI Tianyong  WANG Xumin  LIU Cui  REN Lei  TANG Xuexi  LIU Tao 《海洋学报(英文版)》2014,33(9):139-151

In the past two decades, many studies have focused on the classification within genus Laminaria, ultimately trying to divide it into two subgroups or genera: Laminaria and Saccharina. A significant debate still surrounds the question of its division, as the conflicting phylogenetic hypotheses that have resulted from the classification studies are based on different taxon sampling, molecular markers, or analysis methods. It is aimed at elucidate the molecular phylogeny within Laminaria and Saccharina. The nine species of Laminariales are sampled from northern Asia and Europe, and 23 new sequences in the nuclear, plastid, and mitochondrial genomes are determined to identify their taxonomic status. The phylogenetic analyses of 71 species are performed, including representatives from six of the seven families of the order Laminariales, based on three separate data sets. An evidence is provided to strongly support a clear split that maintains the two recognized genera, Laminaria and Saccharina, with Laminaria appearing to be the ancestor group. Further, analyses indicate that all taxa in Saccharina and Laminaria did not form a monophyletic lineage, instead Laminariaceae and Lessoniaceae grouped together interlacedly, and Costariaceae appeared as the sister taxon of the Lessoniaceae–Laminariaceae clade. In the phylogenetic analysis, mitochondrial c oxidase I(COI) sequences appeared to be the most credible molecular marker which was more befitting than nuclear encoded internal transcribed spacer(ITS) and plastid encoded rbcL for establishment of Laminariales systematics. It is the most comprehensive phylogeny of the order Laminariales, and contributes to an enhanced understanding and estimation of the phylogenetic relationships for the economically important seaweeds, Laminaria and Saccharina.  相似文献   

Transcriptome-wide evolutionary analysis on essential brown algae (Phaeophyceae) in China   总被引：1，自引：1，他引：0  

SUN Jing  WANG Liang  WU Shuangxiu  WANG Xumin  XIAO Jingf  CHI Shan  LIU Cui  REN Lufeng  ZHAO Yuhui  LIU Tao  YU Jun 《海洋学报(英文版)》2014,33(2):13-19

  相似文献   

杂色龙虾线粒体基因组全序列的测定与分析     

刘皓  姚维  刘海情  刘楚吾  刘丽 《海洋科学》2014,38(11):16-23

为了解杂色龙虾(Panulirus versicolor)线粒体基因组多态位点及遗传变异,对龙虾类的分类研究奠定基础,作者通过常规PCR步移扩增法获得杂色龙虾线的线粒体基因组全序列,分析了其线粒体基因组的基本特征以及基因排列情况,并结合中国龙虾(Panulirus stimposni)、波纹龙虾(Panulirus homarus)线粒体基因组全序列,综合分析了龙虾属线粒体编码基因多态位点及基因变异状况。结果表明:杂色龙虾线粒体基因组全序列为15 700 bp,由13个蛋白质编码基因、22个t RNA基因、2个r RNA基因和D-loop区组成,保持了泛甲壳动物线粒体基因组的原始排列。杂色龙虾线粒体DNA的碱基组成具有AT偏好性,A+T含量为67%,4种碱基的含量分别为(A=34.6%,T=32.1%,C=20.5%,G=12.8%)。预测了杂色龙虾18个t RNA以及2个r RNA的二级结构。龙虾属线粒体基因组中的nad 2、nad 5基因以及D-loop区的多态位点比例较高,适合作为分子标记用于龙虾类系统进化关系、种内多态性以及遗传分化等方面的研究。  相似文献