Molecular identification of scallop planktonic larvae using species-specific microsatellites   总被引：3，自引：0，他引：3  

ZHAN Aibin  HU Xiaoli  BAO Lisui  LU Wei  PENG Wei  WANG Mingling  HU Jingjie 《海洋学报(英文版)》2008,27(5):134-146

The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However, the larvae identification by morphological characteristics is notoriously difficult, mainly due to the small size （usually being less than 150 μm） and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species, the Zhikong scallop Chlamys farreri, the noble scallop C. nobilis, the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers, which was specified by transferability analyses, assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1% -2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple, inexpensive and robust in identification analyses, and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years.  相似文献   

Preliminary Study on Applicability of Microsatellite DNA Primers from Parasite Protozoa Trypanosoma cruzi in Free-living Protozoa   总被引：1，自引：0，他引：1  

ZHANGWenjing YUYuhe SHENYunfen MIAOWei FENGWeisong 《中国海洋大学学报(英文版)》2004,3(1):80-84

1　Introduction　Generallyknownasacodominantgeneticmarker ,microsatellitehasbeenwidelyusedinstudiesonpopu lationgenetics,high resolutiongenotyping ,genemap ping ,evolution ,linkageanalysis ,conservationbiology ,behaviouralecology ,relationsbetweenparasite…  相似文献   

Spring-spawning herring (Clupea harengus L.) in the southwestern Baltic Sea: do they form genetically distinct spawning waves?     

Hede Jorgensen  Hanne B.; Hansen  Michael Moller; Loeschcke  Volker 《ICES Journal of Marine Science》2005,62(6):1065-1075

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Genetic structure of Octopus vulgaris around the Iberian Peninsula and Canary Islands as indicated by microsatellite DNA variation   总被引：2，自引：0，他引：2  

Cabranes  C.; Fernandez-Rueda  P.; Martinez  J. L. 《ICES Journal of Marine Science》2008,65(1):12-16

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Genetic history of the population of Atlantic salmon, Salmo salar L., under restoration in the Connecticut River, USA     

Ayllon  Fernando; Martinez  Jose L.; Juanes  Francis; Gephard  Stephen; Garcia-Vazquez  Eva 《ICES Journal of Marine Science》2006,63(7):1286-1289

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许氏平鲉(Sebastes schlegeli)微卫星标记开发及野生、养殖群体遗传多样性分析   总被引：1，自引：1，他引：0       下载免费PDF全文

韩承慧  马海涛  姜海滨  刘阳  韩慧宗  王斐 《海洋与湖沼》2016,47(1):213-220

对许氏平鲉进行简化基因组测序,设计微卫星引物200对,可稳定扩增的引物190对,占95%。利用一个荣成野生群体对24个多态性较高的微卫星标记进行了评价,每个位点的等位基因数(Na)为2—21个,观测杂合度(Ho)为0.0417—0.9167,期望杂合度(He)为0.0278—0.9722,多态信息含量(PIC)为0.1948—0.9496,结果显示有20个微卫星位点为中高度多态。利用这些引物对荣成野生群体和烟台养殖群体的遗传多样性进行了比较分析,野生和养殖群体的平均等位基因数(Na)分别为8.5000、6.9583,有效等位基因数(Ne)的均值分别为4.5484、3.6365,期望杂合度(He)均值分别为0.6421、0.5840,多态信息含量均值(PIC)分别为0.6088、0.5490,平均香农-威纳指数均值为1.4605、1.2834,但F检验发现无显著差异,发现两个群体的遗传多样性都处于高度多态水平,但养殖群体遗传多样性水平低于野生群体。本研究结果说明许氏平鲉的人工繁育中,通过使用较大数量的亲本进行繁育可有效防止选育群体的遗传多样性降低,但人工定向选育对选育群体的遗传多样性也产生了一定的影响。Bonferroni校正后在两个群体中各有4个位点偏离Hardy-Weinberg平衡。本研究开发的微卫星标记为许氏平鲉遗传图谱构建、分子标记辅助育种等提供了更多标记选择,对野生和养殖群体遗传多样性分析为下一步的遗传育种提供参考。  相似文献   

巢湖沉积植硅体组合及中全新世以来的环境演变   总被引：1，自引：0，他引：1  

范斌  许世远  俞立中  蒋辉  冉莉华 《湖泊科学》2006,18(3):273-279

尽管以前对眼虫进行过大量的形态发育研究和基于核糖体RNA基因的系统发育分析,但对于株系之间的关系仍然知之甚少．因其形态特征有限并且易变,很难鉴定眼虫的相似种和同种内不同的株．作者利用微卫星DNA指纹图谱,在七株眼虫中扩增了七个微卫星DNA位点,成功扩增的六个微卫星引物都得到了四到八个条带．从微卫星DNA指纹图谱计算得到的相似性系数范围从0．000到0．957．根据相似性系数得到的树状结构,七株眼虫在距离为0．9346处分为三支:E．mutabilis,E．intermedia和E．gracilis．其中,五株E．gracilis分为两组:来自日本的和美国的．不同地区的株得到不同的基因型,并初步分析了它们之间的关系．研究表明七株眼虫根据微卫星DNA指纹图谱被明显区分开．微卫星DNA指纹图谱具有很高的分辨率,是鉴定和区分原生动物相似种和同种内不同株的一种有用的新方法．  相似文献   

红鳍东方鲀(Takifugu rubripes)耐低温相关微卫星标记的初步筛选     

袁晨浩  刘志峰  马爱军 《海洋科学》2022,46(2):97-104

采用微卫星结合混合群体分离分析法技术（SSR-BSA）对红鳍东方鲀群体进行耐低温相关微卫星标记的筛选。首先对300尾红鳍东方鲀幼鱼进行低温处理,分别获得34尾耐低温（S组）和不耐低温个体（D组）。分别在两组中随机挑选15尾提取基因组DNA,构建耐低温和不耐低温DNA混池,然后用148对微卫星引物对其进行扫描。结果发现4个标记（fms45、fms82、fms100和fms182）在耐低温和不耐低温DNA混池中扩增出差异条带。用S组和D组全部个体对4个标记进行单个体验证,结果显示由fms100扩增,携带有116 bp条带的个体在S组和D组的出现频率分别是53%和18%,132 bp条带的出现频率分别是59%（S组）、24%（D组）;由fms182扩增,携带有125 bp的个体在S组和D组出现的频率分别是12%和35%,经卡方检验P值均小于0.05,差异显著。本文关于红鳍东方鲀耐低温分子标记的报道,为研究红鳍东方鲀耐低温的遗传基础以及相关分子机制提供了依据,也为开展红鳍东方鲀耐低温分子标记辅助选育提供了良好的基础。  相似文献   

红鳍东方鲀(Takifugu rubripes)性别差异微卫星标记的筛选     

岳亮  马爱军  赵艳飞  王新安  何伟国  孟雪松  翟介明  刘圣聪 《海洋与湖沼》2014,45(1):172-177

采用以BSA为基础的微卫星标记技术对红鳍东方鲀(Takifugu rubripes)雌、雄群体进行性别差异标记筛选的研究。用雌、雄各30个个体构建雌、雄基因池,利用66对微卫星引物扫描雌、雄基因池。在雌、雄基因池中扩增出差异条带的引物有8对。用两个各包括30个雌、雄个体的群体对这8对引物进行两轮个体验证。结果表明,引物f383在两个雌、雄群体中扩增出的差异条带与性别都呈极显著相关性(r分别为0.710和0.673)(P0.01),f383是与红鳍东方鲀雄性呈正相关的微卫星标记。红鳍东方鲀性别差异微卫星标记的获得,为其性别相关基因的克隆和性别决定机制的研究提供理论基础。  相似文献   

Analysis of genetic diversity and differentiation of seven stocks of Litopenaeus vannamei using microsatellite markers     

Kai Zhang  Weiji Wang  Weiya Li  Quanqi Zhang  Jie Kong 《中国海洋大学学报(英文版)》2014,13(4):647-656

Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content(PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles(Na) and effective alleles(Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity(Ho) values were lower than the expected heterozygosity(He) values(0.526–0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. Fis values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise Fst values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks(11.3%) was much lower than that within stocks(88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.  相似文献