易盐渍区粘土夹层对土壤水盐运动的影响特征   总被引：2，自引：0，他引：2       下载免费PDF全文

余世鹏  杨劲松  刘广明 《水科学进展》2011,22(4):495-501

针对黄淮海冲积平原土壤剖面中粘土夹层普遍存在的现象,在典型易盐渍区开展模拟易盐农田常规种植条件下水盐运移的大型土柱实验,基于长达20年的长系列监测数据分析,系统研究易盐渍区不同土体构型和地下水位等代表性条件下的土壤水盐运移规律和粘土夹层的影响特征.研究结果表明:粘土层有良好的保水和隔盐能力,尤其对表土积盐的抑制效果显著...  相似文献   

http://www.sciencedirect.com/science/article/pii/S167498711100079X   总被引：1，自引：0，他引：1  

H.A. Wanas 《地学前缘(英文版)》2011,2(4):491-507

Lower Paleozoic rocks exposed in various regions of Egypt (south central Sinai, north Eastern Desert and southwest Western Desert), in addition to occurring in the subsurface such as north Western Desert and the Gulf of Suez. The Lower Paleozoic rocks in Egypt include surface and subsurface rock units of formational status. The surface rock units are the Taba, Araba and Naqus formations. The subsurface rock units include the Shifa, Kohla and Basur formations. The Infracambrian Taba Formation has been discovered recently in the outcrops of the south eastern Sinai in the Taba-Ras El-Naqab area. It is missing and/or not recognized in the subsurface. The Taba Formation consists mainly of reddish brown, unfossiliferous gravelly fine-to medium-grained sandstones cemented by kaolinite and have subordinate beds of paleosols. The Cambrian Araba Formation and its subsurface equivalent (the Shifa Formation) are essentially composed of reddish brown, fine-grained laminated sandstone and siltstone with abundant Skolithos and Cruziana sp. In contrast, the Ordovician-Silurian Naqus Formation and its subsurface equivalents (Kohla Formation and Basur Formation) are mainly composed of white, unfossiliferous, cross-bedded, medium- to coarse-grained sandstones with haphazardly distributed pebbles and cobbles. Sedimentological analysis indicates that the Araba Formation and its equivalents were deposited in a marginal-marine environment, whereas the Naqus Formation and its equivalents were laid down in a fluvio-glacial environment. Integrated stratigraphic and sedimentological studies of the Lower Paleozoic rocks permit reconstruction of the paleogeography of Egypt at that time. Egypt has been largely controlled since the Cambrian by the pre-existing structural framework of the pre-Phanerozoic basement rocks inherited from the Late Proterozoic Pan-African event. Additionally, sedimentation processes were controlled during Cambro-Ordovician times by tectonic movements, whereas glacio-eustatic control predominated during the Late Ordovician-Silurian Period. These studies suggest that most areas of Egypt were exposed lands with episodically transgression by epicontinental seas related to the paleo-Tethys. These lands formed a part of a stable subsiding shelf at the northern Gondwana margin.  相似文献   

坛紫菜磷酸烯醇式丙酮酸羧化酶基因的克隆与序列分析          下载免费PDF全文

张晓娟  王广策  何林文  陈昌生 《海洋科学》2011,35(4):70-76

采用同源克隆的方法和RACE技术,从坛紫菜cDNA中获得了磷酸烯醇式丙酮酸羧化酶(PEPC)的序列,该段序列含2538个核苷酸包含一个终止密码子TAG和191 bp的3'端非编码区(UTR),可编码846个氨基酸.该段序列中无跨膜结构和信号肽序列,推导的氨基酸序列中C末端第813位氨基酸是丙氨酸(A),表明是一个C<,...  相似文献   

矛尾复虾虎鱼溃疡病病原创伤弧菌的鉴定   总被引：1，自引：0，他引：1  

毕可然  张晓君  梁利国  阎斌伦 《海洋科学》2011,35(7):20-25

2009年8月江苏连云港赣榆县多家虾蟹养殖塘混养的矛尾复虾虎鱼（砂nechogobius hasta）大量死亡．从病鱼深层溃烂组织及肝脏中分离出大量优势生长的细菌,人工感染试验证明分离菌对虾虎鱼的半数致死量（LD,0）为1．63×10^6cfu／g。对分离菌进行了形态特征、理化特性和分子生物学等方面的分析。一方面．分离...  相似文献   

杜氏盐藻叶绿体ATP合酶α亚单位基因启动子的克隆及初步功能分析     

谷辉辉  冯书营  潘卫东  李杰  薛乐勋 《海洋科学》2011,35(9):18-23

采用DraⅠ,EcoRⅤ,PvuⅡ,ScaⅠ,SmaⅠ和StuⅠ六种内切酶消化杜氏盐藻（Dunaliella salina）叶绿体基因组DNA,与相应DNA接头连接,构建成无载体连接的盐藻叶绿体Genome Walker DNA文库。利用长距离PCR（long distance-polymerase chain rea...  相似文献   

第1期     

《台湾海峡》2011,(4):595-596

论文与报告基于连续沥取的富钴结壳成凶机制的探讨………………………………………………………………………………蔡毅华,黄奕普,邢娜（1）饥饿对斜带石斑鱼稚鱼胃肠道内分泌细胞的形态、定位和分布密度的影响…………………………………………………翁幼竹,方永强（10）地芽孢杆菌属DYth03DNA聚合酶基因的克隆、表达及性质分析……………………………………………………罗淑娅,李侃,徐丽美（17）杂色鲍血蓝蛋白HtHI基因第16内含子SNP密度的分析…………………………………………梁翠,陈军,游伟伟,王德祥,柯才焕（21）大亚湾大鹏澳海域表层沉积物中浮游植物休眠体集群的季节变化…………………………………………付永虎,王朝晖,康伟,杨宇峰（27）  相似文献   

杂色鲍血蓝蛋白HtH1基因第16内含子SNP密度的分析     

梁翠  陈军  游伟伟  王德祥  柯才焕 《台湾海峡》2011,30(1):21-26

单核苷酸多态性（SNPs）是继RFLP和SSR多态性标记之后的新一代遗传标记系统.本文采用EPIC的方法,分析了45个杂色鲍样品的血蓝蛋白HtH1基因第16内含子（H1 intron 16）的SNP数据.这45个个体分别来自日本、台湾和汕头3个不同海域的野生或养殖群体.日本群体的血蓝蛋白HtH1基因第16内含子呈现出长度多态性,共有3种不同长度,分别是1 900、780、440 bp,测序结果表明3个等位基因可以互相比对;而台湾和汕头杂色鲍养殖群体的均为1 900 bp.初步研究结果表明日本杂色鲍个体大约96 bp长的片段含有1个自身杂合SNP位点;台湾杂色鲍个体约133 bp含有1个自身杂合SNP位点;汕头杂色鲍个体约153 bp含有1个自身杂合SNP位点.13个台湾杂色鲍个体包含的SNP密度为约31 bp含有1个SNP;23个汕头杂色鲍个体包含的SNP密度为约21 bp含有1个SNP.这些数据表明现有的杂色鲍养殖群体包含了丰富的SNP位点,利用SNP分子标记进行高密度遗传图谱的构建是可行的.  相似文献   

羧甲基壳聚糖对肿瘤生长的影响研究     

刘万顺  郑美玲  韩宝芹  亢寿海  李南 《中国海洋大学学报(自然科学版)》2011,41(9):36-40

研究评价了不同分子量及不同取代度的3种羧甲基壳聚糖(CM-CTS)对肿瘤生长的影响。用MTT比色法测定3种CM-CTS对人正常肝细胞L02及3株肿瘤细胞:Bel-7402、SGC-7901及Hela生长的影响;ELISA方法检测3种CM-CTS对2种肝细胞L02及Bel-7402分泌TGF-α及VEGF水平的影响;建立小鼠移植性肿瘤Heps模型,腹腔注射分子量最大的CM-Ⅰ,研究CM-CTS的抑瘤作用。研究结果表明,分子量及取代度不同的3种CM-CTS在0.05~0.8 mg/mL浓度范围内,对L02细胞增殖具有显著促进作用,对Bel-7402、SGC-7901及Hela细胞的增殖则具有一定的抑制作用;3种CM-CTS能提高L02细胞TGF-α分泌水平,抑制Bel-7402细胞分泌TGF-α及VEGF水平;在动物水平上,CM-CTS能抑制小鼠Heps肿瘤生长,且量效关系显著。说明不同分子量及取代度的3种羧甲基壳聚糖对肿瘤生长具一定的抑制作用。  相似文献   

Cold-adaptive alkaline protease from the psychrophilic Planomicrobium sp.547: enzyme characterization and gene cloning     

YANG XiangSheng  CHEN XingLin  XU XianZhong  ZENG RunYing 《极地研究(英文版)》2011,22(1):49-54

A psychrophilic bacterium strain 547 producing cold-adaptive alkaline protease was isolated from the deep sea sediment of Prydz Bay, Antarctica. The organism was identified as a Planomicrobium species by 16S rRNA analysis. The optimal and highest growth temperatures for strain 547 were 15℃ and 30℃, respectively. The extracellular protease was purified by ammonium sulfate precipitation and DEAE cellulose-52 chromatography. The optimal temperature and pH for the activity of the purified enzyme were 35 ℃ and pH 9.0, respectively. The enzyme retained approximately 40% of its activity after 2 h of incubation at 50℃. The enzymatic activity was inhibited by 1 mmol/L phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), indicating that it was a serine protease. The presence of Ca2+ and Mn2+ increased the activity of the enzyme. The protease gene with a size of 1 269 bp was cloned from Planomicrobium sp. 547 using primers designed based on the conserved sequences of proteases in GenBank. The Planomicrobium sp. 547 protease contained a domain belonging to the peptidase S8 family, which has a length of 309 amino acid (AA) residues. The alignment and phylogenetic analysis of the AA sequence indicated that the protease belonged to the subtilisin family.  相似文献   

鱼类DMRT基因的研究进展     

曹谨玲  陈剑杰  甘西  罗永巨 《广东海洋大学学报》2011,31(1):94-98

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