排序方式： 共有132条查询结果，搜索用时 250 毫秒
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夏牙鲆(♂)与牙鲆(♀)人工杂交的细胞遗传学初步研究 总被引：6，自引：0，他引：6
于2004年11～12月在青岛薛家岛养鱼场采用夏牙鲆(Paralichthysdentatus)的精子与牙鲆(Paralichthysolivaceus)的卵子实施了多次人工杂交实验,并进行了父本母本鱼类个体、子代的染色体制片观察和细胞中DNA相对含量的流式细胞仪检测。结果表明,夏牙鲆染色体数为48,核型为2n=48t、臂数NF=48;子代的染色体数和核型与父母本的一样,也为2n=48t;子代的细胞中DNA的相对含量也表明其为二倍体。因此,可以明确其为杂交的后代,而不是雌核发育。 相似文献
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养殖牙鲆鳗弧菌疫苗的研究 总被引：13，自引：0，他引：13
以牙鲆病原性鳗弧菌M3为抗原,制备了细胞（CV）、细胞－胞外产物（CEV）、脂多糖（LPS）三种疫苗,通过浸泡和注射两种途径对牙鲆进行两次免疫,检测疫苗的免疫保护效果。免疫后4周可检测到免疫组牙鲆具有明显的凝集抗体效价,其中注射组效价显著高于浸泡组,而对照组变化不明显。与对照组相比,免疫组牙鲆获得良好的免疫保护力,其中注射免疫组高于浸泡组。在注射免疫途径中,以CEV的免疫保护效果最好;在浸泡免疫途径中,以CEV和LPS的免疫保护效果最好。结果表明鳗弧菌M3的细胞－胞外产物苗有显著的免疫保护作用。 相似文献
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脊椎动物胚胎发育过程中，经卵裂和囊胚期积累了一定数量的细胞后，在原肠期开始出现明显的细胞运动，囊胚表层的细胞通过一个称为组织者(Spemann and Mongald’s organizer)的结构进入胚胎的内部，经过内陷、内卷、汇聚、延伸等一系列细胞运动，按照既定的方向迁移到达目的地，参与构建各个胚层，这一过程称为形态发生运动。形态发生运动是脊椎动物胚胎发育必不可少的重要步骤，在这一过程中，特定的单个细胞或成群细胞的定向迁移，对于三个胚层(外胚层、中胚层、内胚层)，以及由这些胚层所产生的特定组织器官的发育与分化，具有决定性影响
从发育进化角度讲，作为脊椎动物与无脊椎动物过渡类型，一个世纪以来，文昌鱼一直是发育学研究的重要模式生物。其中最重要的发现之一是童第周等做出的关于文昌鱼胚胎调整性的研究(Tung et al.，1958，1960，1965)，他们利用卵裂球的分离和配合实验证明了文昌鱼胚胎在发育过程中具有调整能力，各部分有明显的相互作用。但关于文昌鱼形态发生运动的研究仅限于形态描述(Conkjin，1932; Hirakow et al.，1991)，对于文昌鱼形态发生过程中细胞运动机制依然缺乏深入了解。 相似文献
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牙鲆不同组织中抗蛋白酶物质活性研究 总被引：2，自引：0，他引：2
病原菌在侵袭宿主时 ,往往要分泌一些胞外酶 ,这些酶在感染过程中能够协助病原菌抗吞噬或进行扩散［１］。Ｉｎｇｌｉｓ等１９９３年认为 ,一些海洋中生活的细菌在侵袭鱼体时 ,同样能够分泌多种蛋白酶。实验表明 ,这些侵袭性酶的活性能够被鱼体内一些抗蛋白酶活性物质所抑制。通过检测这些物质对于胰蛋白酶的抑制作用 ,可以定量地测定这些抗蛋白酶物质的活性［２］。本实验以１龄牙鲆为实验对象 ,检测了牙鲆体表黏液、腮、血清中抗蛋白酶物质的分布和活性。１材料和方法１．１牙鲆的饲养实验用牙鲆由中国科学院海洋研究所实验海洋生物学开放… 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｃｒｅａｓｉｎｇａｔｔｅｎｔｉｏｎｗａｓｆｏｃｕｓｅｄｏｎｔｈｅｎｅｅｄｔｏｓｔｕｄｙｔｈｅｂａｃｔｅｒｉｏｌｏｇｙｏｆｍａｒｉｎｅｆｉｓｈｉｎＣｈｉｎａ ,ｂｅｃａｕｓｅｏｆｂａｃｔｅｒｉａａｓｓｏｃｉａｔｉｏｎｗｉｔｈｈｉｇｈｍｏｒｔａｌｉｔｙｉｎｍａｒｉｎｅｆｉｓｈｃｕｌｔｕｒｅａｎｄｃｏｎｓｅｑｕｅｎｔｅｃｏｎｏｍｉｃｌｏｓｓｔｏｆｉｓｈｆａｒｍｓ (Ｄｅｎｇｅｔａｌ.,1 996;Ｌｉｎｅｔａｌ.,1 999) .Ｖｉｂｒｉｏｇｅｎｕｓｂａｃｔｅｒｉａｈａｖｅｅｍｅｒｇｅｄａｓｔ… 相似文献
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Recombinant eel GH and yeast containing chinook salmon growth hormone (reGHand rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer ma-trix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containin greGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium algi-nate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulato fish growth. 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＴｒｉｐｌｏｉｄｙｉｎｄｕｃｔｉｏｎａｎｄｇｙｎｏｇｅｎｅｔｉｃｄｉｐｌｏｉｄｉｎｄｕｃｔｉｏｎａｓｍａｉｎｐａｒｔｓｏｆｃｈｒｏｍｏｓｏｍａｌｍａｎｉｐｕｌａ ｔｉｏｎｈａｖｅｂｅｅｎｐｌａｙｉｎｇｍｏｒｅａｎｄｍｏｒｅｉｍｐｏｒｔａｎｔｒｏｌｅｓｉｎｍｏｄｅｒｎｆｉｓｈｂｒｅｅｄｉｎｇ .Ｍｏｓｔｔｒｉｐｌｏｉｄｆｉｓｈａｒｅｓｔｅｒｉｌｅａｎｄｔｈｅｉｒｒｅｄｕｃｅｄｇｏｎａｄａｌｄｅｖｅｌｏｐｍｅｎｔｒｅｓｕｌｔｓｉｎｉｍｐｒｏｖｅｍｅｎｔｏｆｇｒｏｗｔｈｒａｔｅａ… 相似文献
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Neuropeptide Y(NPY) is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study,we focused on NPY m RNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY m RNA levels were analyzed in different tissues and a high level of expression was detected in the brain. We also demonstrated a correlation between NPY expression levels in the brain and feeding schedule. NPY expression levels in olive flounder maintained on a daily scheduled feeding regimen increased shortly before feeding and decreased after the scheduled feeding time. Compared with the-1 h group before feeding,NPY expression in the 3 h group after feeding decreased significantly( P <0.05). Food deprivation led to an 81.7% decrease in NPY m RNA levels in the 24 h fasted group(P <0.05) and a 91.7% decrease in the 48 h fasted group(P <0.05). Therefore,our study demonstrates that NPY expression is associated with food intake in olive flounder. This result reveals the function of NPY in regulating food intake and its potential importance in olive flounder aquaculture. 相似文献
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Steroid sex hormones,such as estradiol-17β(E2)and testosterone(T),are important regulators of sex change in fish.In this study,we examined the effects of E2 treatment on the dynamics of E2 and T during gonadal differentiation in the olive flounder Paralichthys olivaceus using histology and radioimmunoassay(RIA).Flounder larvae were divided into five groups(G0–G4),and fed with 0 (control),0.2,2,20 and 100 mg E2/kg feed from 35 to 110 day post hatching(dph).Fish growth in the G1 and G2 groups was not significantly different from that of the control group(P0.05),while fish in the G3 and G4 groups were less active and showed growth depression and high mortality.The gonads of fish in the G3 and G4 groups were smaller and surrounded by hyperplastic connective tissue.The frequency of females in the G0–G4 groups was 54.5%,75.0%,100%,100%and 93.3%,respectively.The RIA analyses of E2 and T showed that T levels decreased during gonadal differentiation,and increased slightly at the onset of ovarian differentiation,while E2 levels increased gradually and peaked at the onset of ovarian differentiation in the control group.In the E2-treated groups,T levels decreased before the onset of ovarian differentiation.E2 levels were high on the 48 dph,but declined to a lower level on the 54 dph, and then increased gradually during gonadal differentiation.And a sharp increase of E2 levels were observed in all E2-treated groups at the onset of ovarian differentiation.The data suggest that T and E2 play important roles during gonadal differentiation,and an E2 dose of 2 mg/kg feed could induce sex reversal in P.olivaceus. 相似文献