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91.
93.
人工诱导雌核发育牙鲆的染色体及核型证明 总被引：6，自引：1，他引：5
于1994-1996年,分别在威海海洋渔业捕捞公司（石岛）和鸿洋实业总公司龙须岛育苗场采集人工培育的3－5龄牙鲆亲鱼,采用紫外线照射法使牙鲆精子遗传物质失活,并用冷休克法抑制受精卵第二极体释放,从而获得雌核发育二倍体牙好。原肠期采用空气干燥法、Giemsa染色,得到雌核发育二倍体、正常二倍体及单倍体的染色体制片,进行染色体和核型的分析。结果表明,牙鲆的雌核发育二倍体和正常二倍体的染色体数均为2n=48,核型为48t,即48条端部着色点染色体,臂数NF=48,两者的核型设有明显差异;单倍体为24条端部着丝点染色体;在3个组别中,第一号染色体上都有一明显的次缢痕。雌核发育二倍体牙鲆的诱导率为98％。 相似文献
94.
环境因子对牙鲆精子运动能力的影响 总被引：8，自引：1，他引：7
于1997年5-6月,在山东荣成市石岛养殖场采用挤压法收集牙鲆亲鱼的精子,利用显微镜观察精子的活力,研究外界环境因子的变化对牙鲆精子运动能力的影响。结果表明,将蔗糖、NaCl、KCl、MgCl2、CaCl2等溶解于去离子水中,制成不同浓度即具有不同渗透压的溶液,不同渗透压的溶液对精子运动的诱导结果不同。EDTANa2溶液不能诱导精子运动。在人工海水中加入一定量的EDTANa2后,可使原先运动的精子静止;在该溶液中加入新的Ca2 后,将不再诱导原来受到EDTANa2抑制的精子。如用MgSO4代替溶液中的CaCl2,可诱导精子运动;而当用MgCl2代替溶液中的CaCl2,以及用CaCl2或NaCl代替MgSO4时,溶液即失去诱导精子运动的能力。在pH＝4.0-9、5的海水中,牙解精子均可正常运动。 相似文献
95.
ＩＮＴＲＯＤＵＣＴＩＯＮＢｉｌｌａｒｄ ( 1 986)ｓｔｕｄｉｅｄｔｈｅｓｐｅｒｍａｔｏｚｏｏｎｕｌｔｒａｓｔｒｕｃｔｕｒｅｓｉｎｔｅｌｅｏｓｔｓａｎｄｆｏｕｎｄｄｉｓｔｉｎｃｔｄｉｆｆｅｒｅｎｃｅｓａ ｍｏｎｇｄｉｆｆｅｒｅｎｔｓｐｅｃｉｅｓ.Ｓｔｕｄｙｏｎｓｐｅｒｍａｔｏｚｏｏｎｍｏｒｐｈｏｌｏｇｙａｎｄｆｕｎｃｔｉｏｎｉｓａｃｈａｌｌｅｎｇｅｂｅｃａｕｓｅｏｆｔｈｅｓｐｅｒｍ’ｓｓｐｅｃｉａｌｅｎｖｉｒｏｎｍｅｎｔ (Ｇａｇｅｅｔａｌ.,1 998;ＷａｎｇａｎｄＺｈａｎｇ,1 999) .Ｓｉｎｃｅｌｅｆｔ ｅｙ… 相似文献
96.
STUDY ON EMBRYONIC DEVELOPMENT AND EARLY GROWTH OF TRIPLOID AND GYNOGENETIC DIPLOID LEFI—EYED FLOUNDER, PARALICHTHYS OLIVACEUS （T. et S. ） 总被引：1，自引：0，他引：1
The early effects of chromosomal manipulation of eggs and sperm on the yields of trip-loid and gynogenefic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fen.ilized eggs at 0 - 2℃ for 45 minutes duration 5 minutes after fen.ilization, and the in-duced triploidy rates were 31.2% - 50% and the relative hatching rates were 53.3% - 99%. Gynnge-aetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0 -2℃ for 45 minutes duration 5 minutes after fertihzation. The induced gynogenetic diploid rates and the relative hatching rates were 94 % - 96 % and 48.5 % - 68.5 % respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae ac-cording to Student‘s t-test (α = 0.05). The relationship between the early growth of the triploid experi-mental group larvae and that of gynogenetic diploid experimental group larvae was also studied. 相似文献
97.
Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus： karyotype, allozyme and RAPD analyses 总被引：2，自引：0，他引：2
The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types
of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species.
Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no
difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also
studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal
and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above
38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation
could inherit a little more genetic materials from paternal fish than that from maternal fish.
Supported by National Natural Science Foundation of China (No. 30571445), National High-Technology Research and Development
Program (863 Program, No. 2006AA10A404), and Project from the Ministry of Science and Technology of China (No. 2006DKA30470-017) 相似文献
98.
ＩＮＴＲＯＤＵＣＴＩＯＮＩｔｉｓｗｅｌｌｅｓｔａｂｌｉｓｈｅｄｔｈａｔｉｎｍａｍｍａｌｓ,ｇｒｏｗｔｈｈｏｒｍｏｎｅ (ＧＨ)ｅｌｉｃｉｔｓｉｔｓｇｒｏｗｔｈ ｐｒｏｍｏｔｉｎｇａｃ ｔｉｏｎｉｎｄｉｒｅｃｔｌｙｔｈｒｏｕｇｈｔｈｅｉｎｓｕｌｉｎ ｌｉｋｅｇｒｏｗｔｈｆａｃｔｏｒ Ｉ(ＩＧＦ Ｉ)ｐｒｏｄｕｃｅｄｍａｉｎｌｙｉｎｔｈｅｌｉｖｅｒ.Ａｓｆｏｒｔｅｌｅｏｓｔｆｉｓｈｅｓ,Ｉｎｕｉｅｔａｌ.(1 985 )ｒｅｐｏｒｔｅｄｓｔｉｍｕｌａｔｉｎｇｉｎｖｉｖｏａｎｄｉｎｖｉｔｒｏｅｆｆｅｃｔｓｏｆｏｖｉｎｅＧＨ… 相似文献
99.
文昌鱼(Branchiostoma belcheri tsingtauense)是进化发育生物学研究的重要模式生物,文昌鱼胚胎整装荧光原位杂交(WFISH,whole-mounted fluorescent in situ hybridization)技术将有助于鉴定文昌鱼胚胎发育过程中具有重要调控作用的功能基因。报告了文昌鱼胚胎整装荧光原位杂交技术,用以快速灵敏分析特定基因在文昌鱼胚胎发育过程中的时空表达图式。用体外转录合成的地高辛标记文昌鱼FGFR基因的反义RNA探针,检测到FGFR在文昌鱼原肠胚中表达于发生内陷的中内胚层细胞中,而预定发育为外胚层的细胞不表达FGFR。 相似文献
100.
Proteins of the DYRK (dual-specificity tyrosine-phosphorylation-regulated kinase) family are characterized by the presence
of a conserved kinase domain and N-terminal DH box. DYRK2 is involved in regulating key developmental and cellular processes, such as neurogenesis, cell proliferation, cytokinesis,
and cellular differentiation. Herein, we report that the ortholog of DYRK2 found in zebrafish shares about 70% identity with that of human, mouse, and chick. RT-PCR showed that DYRK2 is expressed maternally and zygotically. In-situ hybridization results show that DYRK2 is expressed in somite cells that will develop into muscles. Our results provide preliminary evidence for investigating the
in-vivo function of DYRK2 in zebrafish muscle development. 相似文献