Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析          下载免费PDF全文

武翠玲  宋英达  高慧芳  张廉芸  任晨霞 《盐湖研究》2020,28(1)

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性，属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高，α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高，且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸，并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示，α螺旋和β片层围成了蛋白酶的催化腔，EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体，且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性，并有助于其催化功能的发挥，为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。  相似文献   

Seismic design of floor–wall joints of multi-storey CLT buildings to comply with regularity in elevation     

Davide Trutalli  Luca Pozza 《Bulletin of Earthquake Engineering》2018,16(1):183-201

The effects of irregularity in elevation of cross-laminated timber buildings have not been fully analysed in literature to provide useful information for the design. In this work, a number of building configurations, regular or irregular in elevation, characterized by a different arrangement per storey of the floor–wall joints have been analysed by means of non-linear dynamic analyses. Comparative results in terms of ratio between the behaviour q-factor of the investigated irregular configurations and that of reference regular ones, show that less dissipative capacity can be expected if the building is irregular due to a disequilibrium among storeys between the actual and the required strength provided by the floor–wall joints. A correlation method to estimate the behaviour q-factor for perfectly regular cross-laminated timber buildings is here presented and extended to in-elevation irregular ones. A new empirical formulation to assess the reliable corrective factor accounting for the irregularity in elevation of cross-laminated timber buildings, according to Eurocode 8 provisions, is also proposed. A final discussion about the implications of in-elevation irregularity on the building design is reported.  相似文献   

Seismic performance and damage incurred by monolithic concrete self-centering rocking walls under the effect of axial stress ratio     

Abouzar Jafari  Mohammad Reza Ghasemi  Habib Akbarzadeh Bengar  Behrooz Hassani 《Bulletin of Earthquake Engineering》2018,16(2):831-858

Self-centering rocking walls offer the possibility of minimizing repair costs and downtimes, and also nullify the residual drift after seismic events, thanks to their self-centering properties. In this paper, the effect of axial stress ratio on the behavior of monolithic self-centering rocking walls is investigated by utilizing a developed finite element model. To verify the validity of the finite element model, results and observed damage in the model are compared with those of a full-scale wall test. The axial stress ratio is varied from 0.024 to 0.30 while keeping the other structural parameters constant. For qualitative damage evaluation, the observed damage in the model compared with expected damage states of desired performance levels. In order to evaluate the incurred damage quantitatively, the amount of crushing and damage in the wall is calculated by utilizing several ratios (crushing ratio and damage ratio). Furthermore, seismic response factors (i.e., μ, R and C_d) are calculated for different axial stress ratio values. The obtained results showed that, in order to satisfy the requirements of desired performance levels, the maximum axial stress ratio should be approximately within the range of 0.10–0.15. In addition, the maximum overall damage ratio and crushing ratio are suggested to be less than 5%. For axial stress ratio higher than 0.15, the flag-shaped pattern of hysteresis curves completely disappeared and the variation of displacement ductility is less sensitive to axial stress ratio. Considering the maximum axial stress ratio limited to 0.150, values of 4 and 3.5 are conservatively proposed as a period-independent response modification factor and displacement modification factor of the investigated structural wall, respectively.  相似文献   

The 2014 Earthquake Model of the Middle East: seismogenic sources     

Laurentiu Danciu  Karin Şeşetyan  Mine Demircioglu  Levent Gülen  Mehdi Zare  Roberto Basili  Ata Elias  Shota Adamia  Nino Tsereteli  Hilal Yalçın  Murat Utkucu  Muhammad Asif Khan  Mohammad Sayab  Khaled Hessami  Andrea N. Rovida  Massimiliano Stucchi  Jean-Pierre Burg  Arkady Karakhanian  Hektor Babayan  Mher Avanesyan  Tahir Mammadli  Mahmood Al-Qaryouti  Doğan Kalafat  Otar Varazanashvili  Mustafa Erdik  Domenico Giardini 《Bulletin of Earthquake Engineering》2018,16(8):3465-3496

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Probabilistic seismic hazard assessment for South-Eastern France     

Christophe Martin  Gabriele Ameri  David Baumont  David Carbon  Gloria Senfaute  Jean-Michel Thiry  Ezio Faccioli  Jean Savy 《Bulletin of Earthquake Engineering》2018,16(6):2477-2511

The accurate evaluation and appropriate treatment of uncertainties is of primary importance in modern probabilistic seismic hazard assessment (PSHA). One of the objectives of the SIGMA project was to establish a framework to improve knowledge and data on two target regions characterized by low-to-moderate seismic activity. In this paper, for South-Eastern France, we present the final PSHA performed within the SIGMA project. A new earthquake catalogue for France covering instrumental and historical periods was used for the calculation of the magnitude-frequency distributions. The hazard model incorporates area sources, smoothed seismicity and a 3D faults model. A set of recently developed ground motion prediction equations (GMPEs) from global and regional data, evaluated as adequately representing the ground motion characteristics in the region, was used to calculate the hazard. The magnitude-frequency distributions, maximum magnitude, faults slip rate and style-of-faulting are considered as additional source of epistemic uncertainties. The hazard results for generic rock condition (Vs30 = 800 m/s) are displayed for 20 sites in terms of uniform hazard spectra at two return periods (475 years and 10,000 years). The contributions of the epistemic uncertainties in the ground motion characterizations and in the seismic source characterization to the total hazard uncertainties are analyzed. Finally, we compare the results with existing models developed at national scale in the framework of the first generation of models supporting the Eurocode 8 enforcement, (MEDD 2002 and AFPS06) and at the European scale (within the SHARE project), highlighting significant discrepancies at short return periods.  相似文献   

Assessment of the influence of horizontal diaphragms on the seismic performance of vernacular buildings     

Javier Ortega  Graça Vasconcelos  Hugo Rodrigues  Mariana Correia 《Bulletin of Earthquake Engineering》2018,16(9):3871-3904

The awareness and preservation of the vernacular heritage and traditional construction techniques and materials is crucial as a key element of cultural identity. However, vernacular architecture located in earthquake prone areas can show a particularly poor seismic performance because of inadequate construction practices resulting from economic restraints and lack of resources. The horizontal diaphragms are one of the key aspects influencing the seismic behavior of buildings because of their major role transmitting the seismic actions to the vertical resisting elements of the structure. This paper presents a numerical parametric study adopted to understand the seismic behavior and resisting mechanisms of vernacular buildings according to the type of horizontal diaphragm considered. Detailed finite element modeling and nonlinear static (pushover) analyses were used to perform the thorough parametric study aimed at the evaluation and quantification of the influence of the type of diaphragm in the seismic behavior of vernacular buildings. The reference models used for this study simulate representative rammed earth and stone masonry vernacular buildings commonly found in the South of Portugal. Therefore, this paper also contributes for a better insight of the structural behavior of vernacular earthen and stone masonry typologies under seismic loading.  相似文献   

Experimental study on seismic behavior of Un-Reinforced Masonry (URM) brick walls strengthened with shotcrete     

M. Shabdin  Nader K. A. Attari  M. Zargaran 《Bulletin of Earthquake Engineering》2018,16(9):3931-3956

In this study, the efficiency of conventional shotcrete technique for strengthening of Un-Reinforced Masonry (URM) walls was shown using an experimental program. In addition, in this program the possible benefit of using anchors for connecting the shotcrete reinforcement layer to the R/C foundation was studied. The experimental program consisted of testing five full scale specimens with two different height-to-length aspect ratios and so different failure modes, under in-plane cyclic loading conditions. Two specimens were tested as reference and others were strengthened on a single-face using shotcrete layer. According to the results, strengthening of URM walls using traditional shotcrete approach created a completely stiff panel and prevented the formation of cracks. The failure mode in both reference and strengthened short length walls was rocking and the shotcrete layer could increase the strength capacity, energy dissipation, and stiffness of wall due to yielding and rupture of steel bars anchored to the foundation. On the other hand, in strengthened long length walls, shotcrete layer increased the shear sliding capacity with no or small increasing in their rocking capacity. Therefore, the failure mode of strengthened walls converted from shear sliding to rocking, even in the specimen with anchorage system. The distributed type of anchorage system could not improve the strength capacity of long length wall. Anchorage system was able to improve the out-of-plane performance of strengthened walls.  相似文献   

Deriving fragility functions from bilinearized capacity curves for earthquake scenario modelling using the conditional spectrum     

Clotaire Michel  Helen Crowley  Pia Hannewald  Pierino Lestuzzi  Donat Fäh 《Bulletin of Earthquake Engineering》2018,16(10):4639-4660

The development of fragility curves to perform seismic scenario-based risk assessment requires a fully probabilistic procedure in order to account for uncertainties at each step of the computation. This is especially true when developing fragility curves conditional on an Intensity Measure that is directly available from a ground-motion prediction equation. In this study, we propose a new derivation method that uses realistic spectra instead of design spectral shapes or uniform hazard spectra and allows one to easily account for the features of the site-specific hazard that influences the fragility, without using non-linear dynamic analysis. The proposed method has been applied to typical school building types in the city of Basel (Switzerland) and the results have been compared to the standard practice in Europe. The results confirm that fragility curves are scenario dependent and are particularly sensitive to the magnitude of the earthquake scenario. The same background theory used for the derivation of the fragility curves has allowed an innovative method to be proposed for the conversion of fragility curves to a common IM (i.e. spectral acceleration or PGA). This conversion is the only way direct comparisons of fragility curves can be made and is useful when inter-period correlation cannot be used in scenario loss assessment. Moreover, such conversion is necessary to compare and verify newly developed curves against those from previous studies. Conversion to macroseismic intensity is also relevant for the comparison between mechanical-based and empirical fragility curves, in order to detect possible biases.  相似文献   

Seismic fragility functions for code compliant and non-compliant RC SMRF structures in Pakistan     

Naveed Ahmad  Asif Shahzad  Qaisar Ali  Muhammad Rizwan  Akhtar Naeem Khan 《Bulletin of Earthquake Engineering》2018,16(10):4675-4703

Fragility functions are derived for low-rise code compliant & non-compliant special moment resisting frames (SMRFs). Non-compliant SMRFs those built in low strength concrete and lacking confining ties in joint panel zones, commonly found in developing countries. Shake table tests were performed on single-storey and two-storey 1:3 reduced scale representative frames to understand the damage mechanism and develop deformation-based damage scale. The non-compliant SMRF experienced column flexure cracking, longitudinal bar-slip in beam and observed with cover concrete spalling from the joint panels. The code compliant SMRF experienced flexure cracks in beam/column, and experienced joint cracking under extreme shaking. Numerical modeling technique is developed for inelastic modeling of reinforced concrete frame with beam bar-slip and joint damageability using SeismoStruct. Natural accelerograms were used to analyze the considered frames through incremental dynamic analyses in SeismoStruct. A probabilistic based approach was used to derive fragility functions for the considered frames. An example case study is presented for damageability evaluation of structures for earthquakes of various return periods (43, 72, 475, 2475 years).  相似文献   

Spatial variation and conditional simulation of seismic ground motion     

Gopala Krishna Rodda  Dhiman Basu 《Bulletin of Earthquake Engineering》2018,16(10):4399-4426

Spatially varying ground motion (SVGM) may have influence on certain civil engineering structures with spatially extended superstructure and/or substructures. Conditional simulation of spatially varying ground motion (CSSVGM) may be viewed from two different perspectives. Most procedures available in the literature neglect the spatial variability in auto-spectral density (ASD) and estimate the SVGM through cross-spectral density (CSD) which was computed using the empirical coherency models. This paper proposes a coherency model that accounts for the spatial variability of ASD. A framework has been developed for the CSSVGM, through the mapping of both proposed coherency model and ASD over the footprint of an array. Current framework (existing in the literature) accounts for only the phase variability of SVGM while proposed framework accounts for both phase and amplitude variability. Ground motion generated from both perspectives is then assessed with the data recorded over SMART1 and LSST arrays. For the purpose of assessment, a definition of target spectrum based on the direction of arrival is explored. The effect of choice of coherency model on the simulated spatially varying ground motion is investigated first. Spectra resulting from both the perspectives are assessed against the target spectrum. An attempt has been made to predict the SVGM for a future event using a coherency model calibrated against a past event and an estimate of ASD of the seed ground motion. Finally, the effect of form of ASD (of a seed ground motion) on SVGM simulated is investigated by considering the ASD in different forms. Simulating SVGM through the mapping of both coherency model and ASD seems to be more appropriate than through CSD.  相似文献