基于网络药理学探讨当归芍药散治疗复发性流产的作用机制          下载免费PDF全文

肖遥  李云君 《应用海洋学学报》2020,36(9):158

目的：利用网络药理学技术研究当归芍药散治疗复发性流产（RSA）的潜在作用机制。方法：通过TCMSP数据库寻找、筛选、预测当归芍药散中5味中药的化学活性成分和对应靶点，通过OMIM数据库、GeneCards 数据库获取与RSA相关的疾病靶点；利用Venny对当归芍药散药物靶点、RSA疾病靶点取交集、绘制韦恩图，从而预测当归芍药散-RSA潜在作用靶点；利用Cytoscape软件构建“当归芍药散—成分—RSA—靶点”网络；分别借助String平台、R软件对RSA药物-疾病潜在作用靶点构建蛋白相互作用网络、分析GO 生物过程、分析KEGG 通路。结果：当归芍药散主要通过芍药苷、芍药内酯苷、泽泻醇、儿茶素、川芎内酯A等30个活性成分作用于IL6、AKT1、JUN、TNF、PTGS2等84个与疾病相关的靶点。通过AGE-RAGE信号通路、肿瘤坏死因子信号通路（TNF）、白细胞介素（IL）-17信号通路、TOLL样受体信号通路、脂多糖反应等生物过程和代谢通路，调节MAPK信号通路和NF-κB信号通路，以达到平衡机体TNF、IL等炎性因子含量，避免了过度自身免疫的发生，从而改善妊娠结局。结论：本研究反应了当归芍药散具有多成分、多靶点、多通路的特点，为当归芍药散治疗复发性流产提供新思路和理论依据。  相似文献   

遥感技术在“6·17”丹巴堵江泥石流灾害链灾区应急救援抢险决策中的应用     

唐尧  王立娟  赵娟  张成信 《中国地质调查》2020,7(5):114-122

2020年6月17日,四川丹巴县半扇门镇梅龙沟发生泥石流,阻断小金川河,形成堰塞湖,造成重大财产损失与人员伤亡。为掌握第一手灾情,辅助开展应急救援,以国产高分二号卫星数据为主要数据源,结合无人机航空摄影数据和灾后现场调查等资料,开展了“6·17”丹巴堵江泥石流灾害链灾区应急调查与分析。研究认为: 灾区影响人口约6.5万人; 国网丹巴县供电公司应急救援队与小金县联通通信应急抢险救援队距离最近,半扇门中学等5所学校可作为临时安置避难场所备选; 受影响矿山企业6家、重要水库1座、水电站1座; 泥石流沟总体呈NW—SE向展布,掩没面积约14.27万m²; 堰塞湖湖面面积约1.03 km²,淹没区约49.8万m²,路基路面全毁路段约2.1 km,下游疑似灾害隐患点9处,因灾受损民居25处、桥梁5处; 优选了2条灾后通达性较好的通往泥石流重灾区的救援生命线。借助遥感技术深入分析泥石流灾害链影响人口等灾情先期研判、灾情灾损应急调查及应急救援生命通道优选,对我国西南山区类似条件地质灾害隐患点的应急抢险救援、防灾减灾工作具有重要的指导意义。  相似文献   

青岛近海物理-生物地球化学过程及其生态响应     

韦钦胜  王保栋  姚庆祯  于志刚  傅明珠  孙俊川  许博超  谢琳萍  辛明 《海洋学报(英文版)》2019,38(2):11-23

近些年来，夏季黄海浒苔大规模暴发，并在青岛近岸海域大面积聚集，引起了广泛的关注。本文基于在夏季和冬季所获得的多学科调查资料，重点研究了青岛近海的水文-生物地球化学过程及其生态影响，阐明了该海域物理-化学-生物等多参数之间的耦合响应。研究显示，夏季黄海冷水团的边界可扩展至青岛近岸海域，并在局部涌升至上层水体，形成沿岸上升流；该上升流可对上层营养盐产生一定的补充，进而促进浮游植物的繁殖，并于底层海域对应形成溶解氧（DO）和pH的低值。夏季青岛近海的上升流可能还有利于随南风漂移至此的浒苔的生长，并在一定程度上引起浒苔的局地旺发；同时，夏季该海域特定的锋面系统对浒苔聚集的影响也不容忽视。冬季黄海暖流在苏北浅滩外侧向山东半岛南部海域延伸，扩展至青岛近海的暖水舌与近岸低温水之间的锋面特征明显，而且在向岸暖水与近岸冷水间还对应形成了明显的营养盐和叶绿素（Chl-a）锋面。该项研究从多学科交叉的视角，增进了对青岛近海物理、化学和生物过程之间耦合关系的认识。  相似文献   

Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq,and cross-amplification to related species     

Niu  Sufang  Zhai  Yun  Wu  Renxie  Zhang  Haoran  Tian  Letian  Deng  Jiaxin  Xiao  Yao 《中国海洋湖沼学报》2019,37(1):245-255

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( H_o ) and expected heterozygosity ( H_e ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.

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基于SLAF-seq技术的黑棘鲷微卫星标记开发及其在鲷科鱼类中的通用性研究     

吴仁协  肖瑶  牛素芳  翟云  张浩冉  陈伟勇  黎晓 《海洋与湖沼》2019,50(2):365-377

黑棘鲷(Acanthopagrusschlegelii)是广泛分布于西北太平洋的暖温性中下层经济鱼类,也是我国沿海重要的海洋捕捞鱼类和增养殖对象。然而,目前有关黑棘鲷的微卫星标记研究报道较少,难以对其种质资源状况作出精确评估。本研究采用SLAF-seq技术测序共获得22489个二至六碱基重复的黑棘鲷微卫星序列,短重复序列(二、三碱基)占总微卫星序列的90.8%,长重复序列(四至六碱基)占有9.2%。经过157对随机合成引物的多态性筛选,开发出49个高多态性的黑棘鲷微卫星标记,其中短重复序列位点有25个,长重复序列位点有24个。每个位点的等位基因数(Na)为2—20(均值为8.3),观测杂合度(Ho)和期望杂合度(He)分别为0.097—0.938和0.122—0.922(均值分别为0.663和0.701),多态信息含量(PIC)为0.118—0.897(均值为0.655)。经Bonferroni校正后,有47个位点符合哈迪-温伯格平衡(HWE),各位点间未检测到连锁不平衡现象,仅2个位点偏离HWE。结果表明,所开发的大部分微卫星标记具有高多态性,蕴含的遗传信息含量较为丰富,能够为黑棘鲷的种群遗传资源评估提供数量充足、类型多样的有效分子标记。跨物种扩增结果显示,有43个黑棘鲷微卫星标记可在9种鲷科鱼类中成功扩增,其中28个标记在太平洋棘鲷(Acanthopagruspacificus)、黄鳍棘鲷(Acanthopagruslatus)和澳洲棘鲷(Acanthopagrusaustralis)中具有较好的通用性,2个标记在平鲷(Rhabdosargussarba)、蓝点赤鲷(Pagruscaeruleostictus)、真赤鲷(Pagrusmajor)、二长棘犁齿鲷(Evynnis cardinalis)及黄牙鲷(Dentex hypselosomus)中具有通用性。这些通用性标记可为阐明鲷科属、种间的系统进化关系和棘鲷属鱼类的群体遗传学分析提供新的标记来源和研究角度。  相似文献   

基于污染负荷核算的岷江流域总磷污染成因分析及对策     

温泉  马迎群  时瑶  迟明慧  秦延文  刘志超  杨晨晨 《地学前缘》2020,27(4):332-339

总磷是长江流域水环境污染的首要超标因子,岷江作为长江上游流量最大的支流,总磷污染严重,对长江总磷污染贡献较大。为了解岷江流域总磷污染,采用排污系数法,计算得到2016年岷江流域污染源总磷入河量为1 154 t,以农村生活污染负荷占比最高(51.3%),其次为城镇生活源(28.7%)、农业非点源(8.24%)、工业源(9.57%)、畜禽养殖源(1.21%),城市径流源(0.99%)最低;在空间上岷江流域总磷污染负荷呈中游(64.2%)>下游(32.6%)>上游(3.1%)的特点,与岷江干流总磷浓度变化趋势相符,其中成都市总磷污染负荷最高(51.2%),与区域人口密度高、生产和生活活动密集有关。结合资料收集和现场调查,岷江流域总磷污染成因主要包括农村生活污染治理缺口较大、城镇生活污染处理基础设施建设不足、工业企业密布、部分支流总磷污染严重、水污染治理导向不全面。针对岷江总磷污染负荷分布特征及成因,提出“上游保护优质水体、中游治理重污染水体、下游恢复不达标水体”的分区污染防治对策,统筹流域监管体制机制,强化岷江流域水环境保护和治理。  相似文献   

The Effects of Ocean Acidification on Feeding and Contest Behaviour by the Beadlet Anemone <Emphasis Type="Italic">Actinia equina</Emphasis>     

Tess Olivia Bamber  Angus Charles Jackson  Robert Philip Mansfield 《Ocean Science Journal》2018,53(2):215-224

Increasing concentrations of atmospheric carbon dioxide are causing oceanic pH to decline worldwide, a phenomenon termed ocean acidification. Mounting experimental evidence indicates that near-future levels of CO₂ will affect calcareous invertebrates such as corals, molluscs and gastropods, by reducing their scope for calcification. Despite extensive research into ocean acidification in recent years, the effects on non-calcifying anthozoans, such as sea anemones, remain little explored. In Western Europe, intertidal anemones such as Actinia equina are abundant, lower trophic-level organisms that function as important ecosystem engineers. Changes to behaviours of these simple predators could have implications for intertidal assemblages. This investigation identified the effects of reduced seawater pH on feeding and contest behaviour by A. equina. Video footage was recorded for A. equina feeding at current-day seawater (pH 8.1), and the least (pH 7.9) and most (pH 7.6) severe end-of-century predictions. Footage was also taken of contests over ownership of space between anemones exposed to reduced pH and those that were not. No statistically significant differences were identified in feeding duration or various aspects of contest behaviour including initiating, winning, inflating acrorhagi, inflicting acrorhagial peels and contest duration. Multivariate analyses showed no effect of pH on a combination of these variables. This provides contrast with other studies where anemones with symbiotic algae thrive in areas of natural increased acidity. Thus, novel experiments using intraspecific contests and resource-holding potential may prove an effective approach to understand sub-lethal consequences of ocean acidification for A. equina, other sea anemones and more broadly for marine ecosystems.  相似文献   

An interacting new holographic dark energy in the framework of fractal cosmology     

Ehsan Sadri  Martiros Khurshudyan 《Astrophysics and Space Science》2018,363(11):230

In this paper, we study an interacting holographic dark energy model in the framework of fractal cosmology. The features of fractal cosmology could pass ultraviolet divergencies and also make a better understanding of the universe in different dimensions. We discuss a fractal FRW universe filled with the dark energy and cold dark matter interacting with each other. It is observed that the Hubble parameter embraces the recent observational range while the deceleration parameter demonstrates an accelerating universe and a behavior similar to \(\Lambda \mbox{CDM}\). Plotting the equation of state shows that it lies in phantom region for interaction mode. We use \(\mathit{Om}\)-diagnostic tool and it shows a phantom behavior of dark energy which is a condition of avoiding the formation of black holes. Finally we execute the StateFinder diagnostic pair and all the trajectories for interacting and non-interacting state of the model meet the fixed point \(\Lambda \mbox{CDM}\) at the start of the evolution. A behavior similar to Chaplygin gas also can be observed in statefinder plane. We find that new holographic dark energy model (NHDE) in fractal cosmology expressed the consistent behavior with recent observational data and can be considered as a model to avoid the formation of black holes in comparison with the main model of NHDE in the simple FRW universe. It has also been observed that for the interaction term varying with matter density, the model generates asymptotic de-Sitter solution. However, if the interaction term varies with energy density, then the model shows Big-Rip singularity. Using our modified CAMB code, we observed that the interacting model suppresses the CMB spectrum at low multipoles \(l<50\) and enhances the acoustic peaks. Based on the observational data sets used in this paper and using Metropolis-Hastings method of MCMC numerical calculation, it seems that the best value with \(1\sigma \) and \(2\sigma \) confidence interval are \(\Omega _{m0}=0.278^{+0.008~+0.010} _{-0.007~-0.009}\), \(H_{0}=69.9^{+0.95~+1.57}_{-0.95~-1.57}\), \(r_{c}=0.08^{+0.02~+0.027}_{-0.002~-0.0027}\), \(\beta =0.496^{+0.005~+0.009} _{-0.005~-0.009}\), \(c= 0.691^{+0.024~+0.039}_{-0.025~-0.037}\) and \(b^{2}=0.035\) according to which we find that the proposed model in the presence of interaction is compatible with the recent observational data.  相似文献   

Sunspot Positions and Areas from Observations by Pierre Gassendi     

Mikhail Vokhmyanin  Nadezhda Zolotova 《Solar physics》2018,293(11):150

Solar activity behaviour on the eve of the Maunder minimum may provide important information on the period of further suppression of sunspot population. We analyse sunspot positions and areas in the 1630s extracted from rare drawings published by Pierre Gassendi in Opera Omnia. This work was published in two different editions, the first in Lyon and the second almost 70 years later in Florence. The drawings published in Lyon are found to be slightly different from those published in Florence, which produces a discrepancy in the position of spots of a few degrees, while sunspot group areas may differ by a factor of two. We reveal that the orientation of the drawings in the book is not always the same as might be seen in the telescope. We conjecture that the time of Gassendi’s observations covers the beginning of a new Schwabe cycle in the southern hemisphere. The differential rotation rate in the 1630s is also assessed and discussed.  相似文献   

Low resolution spectroscopy of selected Algol systems     

Shanti Priya Devarapalli  Rukmini Jagirdar  M. Parthasarathy  D. K. Sahu  Vijay Mohan  B. C. Bhatt  Vineet S. Thomas 《Astrophysics and Space Science》2018,363(4):69

The analysis of spectroscopic data for 30 Algol-type binaries is presented. All these systems are short period Algols having primaries with spectral types B and A. Dominant spectral lines were identified for the spectra collected and their equivalent widths were calculated. All the spectra were examined to understand presence of mass transfer, a disk or circumstellar matter and chromospheric emission. We also present first spectroscopic and period study for few Algols and conclude that high resolution spectra within and outside the primary minimum are needed for better understanding of these Algol type close binaries.  相似文献