To enhance the utilization efficiency of farmland irrigation water and reduce the leakage of water conveyance channels, the leakage process of channels was simulated dynamically. The simulated results were compared with data measured in laboratory experiments, and the performance of the model was evaluated. The results indicated that the simulated values of the model were consistent with the observation values, and the R2 values varied between 0.91 and 0.99. In addition, based on the laboratory experiments, a water supply system (Mariotte bottles) and soil box were built using plexiglass. Three influencing factors, namely, the channel form, soil texture and channel cross-sectional area, were varied to observe and calculate the resulting cumulative infiltration amount, infiltration rate and wetting front migration distance. HYDRUS-3D software was used to solve the three-dimensional soil water movement equation under different initial conditions. The results demonstrated that the U-shaped channel was more effective than the trapezoidal channel in increasing the utilization efficiency of the water resources. A U-shaped channel with a small channel cross-sectional area should be adopted and the soil particle size should be prioritized in the construction of water conveyance channels for farmlands. The simulation results were in agreement with the observed results, which indicates that HYDRUS-3D is a reliable tool that can accurately simulate the soil moisture movement in water conveyance channels. The research results can provide a reference for the design and operation of farmland irrigation systems. 相似文献
Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
