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1.
厦门湾沉积物中有害有毒甲藻休眠孢囊及塔玛亚历山大藻的潜在危害 总被引：7，自引：0，他引：7
本工作通过分析厦门湾表层沉积物中的甲藻休眠孢囊，发现了1种有毒塔玛亚历山大藻(Alexandrium tamarense)休眠孢囊，及两种有害甲藻休眠孢囊：锥状斯氏藻(Scrippsiella trochoidea)、具刺膝沟藻(Gonyaulax spinifera)．对塔玛亚历山大藻进行了培养。并初步研究了该藻的毒性及贝类对其毒素的积累．研究结果表明，厦门海域沉积物中的塔玛亚历山犬藻是有毒的．它的较适宜生长环境条件是：水温20～25℃．盐度为25～30，光照度为3000～50001x．塔玛亚历山大藻在每年的4月26日至6月10日、10月11日至11月20日可能会形成有害藻华，危害厦门海域的海洋生物并可能威胁到人类的健康和生命安全． 相似文献
2.
麻痹性贝毒PSP在紫贻贝体内的累积、转化与排出 总被引：13，自引：2，他引：13
于1998年9月在青岛鲁迅公园附近礁石区采集紫贻贝（Mytilus edulis)，采用实验室培养的方法，初步研究了塔玛亚历山大藻（Alexandrium tamarense,ATHK)产生的麻痹性贝毒（Paralytic Shellfish Poison,PSP)在其体内累积、转化与排出的规律。结果表明，在累积实验阶段，紫贻贝内脏的和肌肉中的PSP毒素含量均随实验时间的延长而逐渐增加，累积实验结束时，平均每只贝体内的PSP毒素含量为13.40nmol，毒性水平为12.24ugSTXEq/100g，紫贻贝内脏中的毒素含量远远高于肌肉，内脏中PSP毒素占贝体内PSP毒素总量的97.5%。在8天的排出实验阶段，贝体内的PSP毒素总量呈下降趋势，实验结束时，PSP毒素共排除了约50%，每天排除率约为9%。 相似文献
3.
中国沿海亚历山大藻(Alexandrium)核糖体rDNA部分序列分析及该藻属分子系统进化研究 总被引：4，自引：1，他引：4
分析了几株自南海及东海分离的亚历山大藻的rDNA部分序列信息,其中包括核糖体大亚基(LSU)rDNA的5′端D1-D2区序列,以及5.8SrDNA和ITS区序列;同时也对实验室保种的部分来自其它国家和地区的亚历山大藻相关序列进行了测序和分析,并以此作为序列分析中的参考。采用ClustalX及MEGA2软件对所得到的序列信息进行了综合分析与对比。结果表明,分离自南海的塔玛亚历山大藻(Alexandriumtamarense)和分离自东海的链状亚历山大藻(A.catenella),即便是在ITS区和LSUrDNA等高变区,其序列信息也完全一致。与基因库中搜索到的其它亚历山大藻rDNA序列信息相比较,中国沿海的塔玛/链状亚历山大藻序列更接近于塔玛复合种的“亚洲温带”基因型。对于分离自南海的另外两株未定种的亚历山大藻,通过对比序列信息,发现它们与相关亚历山大藻(A.affine)非常接近。分离于我国台湾地区的微小亚历山大藻(A.minutum)在序列上与分离自新西兰的藻株相似,而与分离自欧洲的微小亚历山大藻藻株相差较大。中国沿海亚历山大藻rDNA序列信息的获得为针对有毒藻种设计特异性核酸探针,发展灵敏快速的生物检测技术奠定了基础。 相似文献
4.
New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellateAlexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected
rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA,
were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report inAlexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp
(44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance,
91% sequence similarity) in comparison withProrocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains.
In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton,A tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic
ambiguities ofAlexandrium genus, particularly of the tamarensis complex. 相似文献
5.
渤海裸甲藻和链状亚历山大藻的麻痹性贝毒毒素分析 总被引：1，自引：0，他引：1
目的:分析渤海裸甲藻(Gymnodinium sp.)和链状亚历山大藻(Alexandrium catenella)的麻痹性贝毒毒素,为渤海天津海域的赤潮研究积累基础数据。方法:通过实验室培养裸甲藻和链状亚历山大藻,选取对数生长期、平台生长期的裸甲藻以及平台生长期的链状亚历山大藻,利用高效液相色谱法(HPLC)对这两种微藻进行麻痹性贝毒(PSP)毒素分析。结果:裸甲藻细胞内不含有麻痹性贝毒(PSP);链状亚历山大藻细胞内含有C毒素和GTX1-4毒素,该微藻每个细胞毒素含量约为10.81 fmol/cell。结论:裸甲藻细胞内虽不含有麻痹性贝毒(PSP),但不能排除其含有其它毒素的可能。链状亚历山大藻细胞内含有麻痹性贝毒(PSP),属于有毒微藻,需要对其进行密切监测。 相似文献
6.
采用麻痹性贝类毒素小白鼠生物测定法 ,研究了在可控生态条件下两株海洋细菌S1 0 、P42 对塔玛亚历山大藻生长及其产毒量的影响。结果表明 ,菌株S1 0 在较高浓度下对藻细胞的生长有明显的抑制作用 ,在较低浓度下抑藻生长作用较弱 ,不同浓度的菌株S1 0 均能有效地抑制藻细胞内麻痹性贝类毒素的产生 ,且在较低浓度下效果较好 ;菌株P42 对该藻的作用恰好与S1 0 相反 ,在较低浓度下明显抑制藻细胞的生长 ,不同浓度的菌株P42 也能有效地抑藻产毒 ,且在较高浓度下作用较明显。实验用的藻株毒力约为 ( 0 .95— 1 2 .1 4)× 1 0 - 6MU/cell,属于低毒藻株 ,该藻株在培养第 1 4天达到毒性最高峰 ,峰值为 1 2 .1 4× 1 0 - 6MU/cell,之后逐渐下降。讨论了海洋细菌在赤潮生物防治中的应用前景 相似文献
7.
A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum. 相似文献
8.
CO2加富对塔玛亚历山大藻叶绿素荧光参数及产毒的影响 总被引：2，自引：2，他引：0
由大气中CO_2浓度升高引起的海洋酸化,是全球性的重大环境问题之一。本研究采用实验生态学的方法,以塔玛亚历山大藻(Alexandrium tamarense)为研究对象,分析其在CO_2加富条件下叶绿素荧光动力学参数及产毒特征的变化。调制叶绿素荧光结果显示,CO_2加富对塔玛亚历山大藻的PSⅡ最大光化学量子产量(Fv/Fm)、最大相对电子传递效率(r ETRmax)有显著影响(P0.05),且随着培养时间的增长Fv/Fm、r ETRmax均降低,对半饱和光强(Ik)、快速光曲线初始斜率(α)却无显著影响(P0.05)。结果说明CO_2加富能促进塔玛亚历山大藻的PSⅡ最大光化学量子产量,提高其最大光能转换效率和相对最大电子传递效率。高效液相色谱法分析结果显示,该株塔玛亚历山大藻主要产漆沟藻毒素1(GTX1)、漆沟藻毒素4(GTX4)、N-磺酰氨甲酰基毒素(C1)及N-磺酰氨甲酰基毒素(C2)四种PSTs毒素,CO_2加富不改变主要麻痹性贝毒(PSTs)的种类组成,但能显著提高氨基甲酸酯类毒素(GTX1、GTX4)产量(P0.05),而降低N-磺酰氨甲酰基类毒素(C1、C2)产量(P0.05),说明加富能使塔玛亚历山大藻所产毒素发生转化,进而影响藻细胞的整体毒性。 相似文献
9.
Distribution of dinoflagellate cysts in Yellow Sea sediments 总被引：1，自引：1，他引：0
To investigate the distribution,abundance,and species composition of dinoflagellate cysts in the Yellow Sea,surface sediment samples were collected at 37 sites,including the Korean dump site.Twenty-one dinoflagellate cyst taxa were identified,with the assemblages dominated mainly by Spiniferites bulloideus,Operculodinium centrocarpum,and cyst of Alexandrium catenella/tamarense type.A high frequency of O.centrocarpum in the Yellow Sea was observed for the first time,and it is likely that this can be attributed to the dynamics of the Yellow Sea Cold Water Mass and the Changjiang(Yangtze) River runoff.Total cyst concentrations ranged from 23 to 48 442 cysts/g dry weight,and high cyst concentrations were recorded adjacent to the dumping site.This result suggests that anthropogenic activities such as ocean dumping stimulate the growth of dinoflagellates in the Yellow Sea,which in turn leads to high levels of dinoflagellate cyst production. 相似文献
10.
A. Lincoln MacKenzie 《新西兰海洋与淡水研究杂志》2014,48(3):430-465
New Zealand's reputation as a supplier of high quality food products is vital to the national economy; international consumers are acutely aware of food safety issues and markets are increasingly demanding higher standards. Filter feeding bivalves are particularly sensitive to the nature of the environment in which they are grown, and quality assurance is a major preoccupation of the shellfish aquaculture industry. With the exception of a couple of incidents, most notably the Gymnodinium catenatum blooms in 2000–2003, paralytic shellfish toxin (PST) contamination has, to date, not had an important effect on the economics and sustainability of the industry. However, the dinoflagellate species responsible for producing these toxins are not uncommon in New Zealand coastal phytoplankton communities, and it is important that awareness of the potential risk is maintained. This review summarises what we know about the causes and incidence of PST contamination from research and monitoring over the last 20 years, since it was first identified in New Zealand. It describes the dynamics of major events and their consequences, and evaluates what is likely to happen in the future as aquaculture expands into new areas with known histories of this problem. 相似文献