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1.
采用PHA、秋水仙素活体注射,取肾细胞空气干燥法制片,研究斑头鱼()和铠平鱼由(♀)的核型。其核型分别为2n=48,7m+33sm+8st,NF=88和2n=48,3m+2st+43t,NF=51。并且这两种鱼的核型中均有1条具次缢痕的染色体,因此形成异形染色体对。这是黄渤海鱼类染色体研究首次发现  相似文献   

2.
采用PHA体内注射肾细胞直接制片法研究青岛近海常见经济鱼类斜带髭鲷和横带髭鲷的核型.得出核型公式前者为:2n=48,2m+8sm+2st+36t,NF=58,后者为2n=48,2m+8sm+14st+24t,NF=58。斜带钱绸核型中t5和t16对染色体及横带货绸核型中t7对染色体为具次缢痕的染色体。两种鱼的核型特点符合典型的高位类群鱼类核型特征。  相似文献   

3.
采用PHA体内注射肾细胞直接法和银染法研究的核型及Ag-NOR带。提出的核型公式为2n=48,2m+8sm十2st+36t,NF=58。核型中第二对端部着丝点染色体(t染色体)为典型的具次缢痕染色体。银染结果显示次缢痕部位为银集核仁组织者区(Ag-Nu-cleolusorganizerregion,Ag-NORs),并表现出数目和结构多态性。此外,还讨论了的不同群体间核型的差异,认为不同群体间存在明显的多态性。  相似文献   

4.
海边香豌豆是生长在青岛海边的野生豆科植物,具有开发前途和应用价值。研究表明,它具有耐盐性、多年生、再生力强、抗逆性强、营养丰富等特点。其核型为K(2n)=14=6m+8sm。  相似文献   

5.
日本沼虾染色体及其核型的研究   总被引:23,自引:0,他引:23  
于1992年3月在上海青县采集日本沼虾,以改进的空气干燥法研究精巢细胞染色体及核型,特别较详细地研究了减数分裂前期I染色体的行为。结果表明,日本沼虾染色体数n=53,2n=104,共分为A,B,C,D4个染色体组;核型组成是N=37M+4ST+11T;初级精母细胞减数分裂前期I可分为细线期/偶线期,粗线期、双线期和终变期5个时期,双线期的二价体存在弥散阶段,减数分裂的同步率高,大多处于双线期,且双  相似文献   

6.
以鳃、肝胰腺、心脏、精巢、成熟卵、各期胚胎及溞状幼体等为材料,采用空气干燥法对日本[虫寻](Charybdis japonica)的染色体数目和核型进行了研究。结果表明,发育期的精巢最适宜进行日本[虫寻]染色体计数,用眼点期之后的胚胎和潘状幼体制片,适宜做核型分析。日本[虫寻]染色体的数目是2n=114,n=57。核型分析显示,日本[虫寻]有中部着丝粒染色体(m)共计17对(第1~17号),亚中部着丝粒染色体(sin)7对(第18~24号),亚端部着丝粒染色体(st)仅1对(第25号),端部着丝粒染色体(t)共计32对(第26~57号)。日本[虫寻]的核型公式为:2n=114—34m+14sm+2st+64t,染色体臂数NF=164,未能发现异形性染色体的存在。  相似文献   

7.
斑尾复鰕虎鱼的染色体研究   总被引:5,自引:0,他引:5  
利用腹腔注射秋水仙素制备肾细胞染色体的方法,对斑尾复鍜虎鱼的染 色体组型和C-带核型进行了研究。结果表明,其二倍体数目为 2n=44,核型为2M +2SM+4ST+34T+XY。X为端着丝粒染色体,Y为中着丝粒染色体。C-带核型显 示,每对染色体的着丝粒均具异染色质带,Y染色体的短臂全部为异染色质。  相似文献   

8.
斑尾复虾虎鱼的染色体研究   总被引:1,自引:0,他引:1  
利用腹腔注射秋水仙素制备肾细胞染色体的方法,对斑尾复虾虎鱼的染色体组型和C-带核型进行了研究。结果表明,其二倍体数目为2n=44,核型为2M+2SM+4ST+34T+XY。Y为端着丝粒染色体,Y为中着丝粒染色体。C-带核型显示,每对染色体的着丝粒均具异染色质,Y染色体的短臂全部为异染色质。  相似文献   

9.
用光镜和电镜技术初步探讨了翡翠贻贝Pernaviridis足丝器(Byssalappratus)的三大组成部分之间的空间结构关系,着重观察足丝柄部发生器(BSG)的片层结构,揭示小片具有典型的胶原原纤维电镜特征。此外,通过足丝丝部的表面结构观察,发现可用众多的突起来解释足丝表面对放射性碘的强机械吸附作用;利用足丝丝部横切片,计算出其长径约105μm,短径约40μm。  相似文献   

10.
翡翠贻贝足丝器的组织结构观察   总被引:1,自引:0,他引:1  
石琼  陈舜华 《热带海洋》1996,15(2):13-16,T002
用光镜和电镜技术初步探讨了翡翠贻贝Perna viridis足丝器的三大组成部分之间的空间结构关系,着重观察足丝柄部发生器(BSG)的片层结构,揭示小片具有黄型的胶原原纤维电镜特征。此外,通过足丝丝部的表面结构观察,发现可用众多的突起来解释足丝表面对放射性碘的强机械吸附作用;利用足丝丝部横切片,计算出其长径约105μm,短径约40μm。  相似文献   

11.
Although the Danish Wadden Sea is of international importance for several bird species, large-scale blue mussel Mytilus edulis fishing took place from 1984–1987, ceasing thereafter due to low mussel stocks. Mussel fishing removes much of the blue mussel biomass, especially larger individuals. Hence we predict that intensive mussel fishing will affect their predators, such as the Eider Somateria mollissima, which is predominantly a blue mussel feeder by, 1) reducing the amount of blue mussels in their diet relative to alternative prey items, 2) exploitation of smaller blue mussel shell classes, 3) loss of body condition, 4) changing feeding distribution to aggregate to the remaining mussel stocks, and 5) decreasing numbers. Before winter 1986/87 blue mussel biomass was estimated at 40,600 tons, decreasing to 15,400 tons in 1987/88 due to mussel fishery. We collected Eiders in both periods to monitor their diet and body mass and used aerial surveys to determine changes in numbers and distribution. Between the two periods, blue mussels declined in the Eiders diet, numbers of Eiders with empty stomachs increased and the mean length of blue mussel taken by Eiders decreased. Eider body condition declined from 1986/87 to 1987/88, mostly the result of the reduction in numbers of individuals with blue mussel remains in their gizzards and in better body condition compared to those taking alternative food items or having empty gizzards. Eiders shifted their distribution from the southern part of the Danish Wadden Sea to the northern part, where the remaining blue mussel stocks were situated. Eider numbers were lowest in 1987/88, the year of lowest blue mussel stocks. We conclude that intensive mussel fishery affected the Eider's diet, reduced their body condition and affected distribution and abundance. The results also showed that availability of blue mussels may have a key role in building up and maintaining body condition in Eiders during winter.  相似文献   

12.
提出以贻贝的外套膜为主要原料,通过多种酶水解及其它工艺处理,研制出贻贝外套膜富硒液。分析表明,提取液富含天然有机硒、锌、铁、铜、锰微量元素及丰富的维吐素A、维生素E、必需氨基酸等多种天然营养物质。  相似文献   

13.
申望  叶茂  王日昕  石戈  赵淑江 《台湾海峡》2011,30(2):250-256
以筛选的2个可鉴别的地中海贻贝(Mytilus galloprovincialis)、厚壳贻贝(M.coruscus)及其杂交后代的PCR标记(扩增核基因Glu-5’特异片段PCR标记Me14/Me17和延伸因子1α第一个内含子区段PCR标记引物EFbisF/EFbisR),对舟山海域3个贻贝主产区(马鞍列岛海域、浪岗山列岛海域、中街山列岛海域)采集的贻贝样本的种类进行鉴别.研究结果显示:100个马鞍列岛海域贻贝个体和30个中街山列岛海域贻贝个体均为厚壳贻贝;而54个浪岗山列岛海域贻贝样本中,16个个体(29.6%)为厚壳贻贝,38个个体(70.4%)为地中海贻贝;3个群体中均未检测到2种贻贝的杂交个体.因此,推测舟山海域地中海贻贝自然分布仅局限于局部海区,但在适宜的海区地中海贻贝可取代厚壳贻贝成为贻贝床的优势物种,舟山海域贻贝床的动态变化值得进一步关注;贻贝样本中没有检测到地中海贻贝与厚壳贻贝的杂交个体,表明调查海域可能没有杂交贻贝分布或数量极少,地中海贻贝通过杂交、基因渐渗污染厚壳贻贝基因组的风险较小.  相似文献   

14.
The aim of this investigation was to develop appropriate methods for quantitative assessment of blue mussel beds. Combined methods of remote sensing, ground truth investigation and sampling in the field were applied to mature and young intertidal mussel beds. Three variables were measured to obtain reliable quantitative estimates of biomass and abundance: the total area covered by a bed; the cover, i.e. the area of mussel-covered patches in relation to the total mussel bed area; and the proportion, i.e. the area covered by mussels within the patches in relation to the total area of mussel patches. Cover and proportion of intertidal blue mussel beds were measured in the field by the transection method. Aerial photographs enabled the total area of the beds to be determined and large-scale surveys of mussel beds to be carried out synchronously. By examining large-scale aerial photographs it was possible also to determine the cover by remote sensing. The proportion could not be derived from aerial photographs, even from pictures at the largest scale tested during this investigation (scale 1:2500). By statistical methods it was estimated that 12 sub-samples (each covering 177 cm2) from mature beds, and 12 sub-samples (each covering 38 cm2) from young beds are needed to obtain reliable data on biomass and abundance.  相似文献   

15.
贻贝营半固着生活,依靠足丝将自己牢固地黏附在底物上.足丝的结构和性能对贻贝的正常生命活动至关重要.为研究热液区极端环境下深海贻贝足丝的结构和性能,采集了印度洋龙旂深海热液区的贻贝(Bathymodiolus marisindicus)足丝,对其力学特性、结构特征和组分进行了分析.力学性能测试发现与近海贻贝(Mytilu...  相似文献   

16.
本文在分析了十足目动物染色体研究现状的基础上,对染色体研究的常规技术(包括G带技术、银染技术)进行改进,并应用于锯缘青蟹染色体研究.主要研究内容为染色体形态和数目、染色体核型、银染特征及减数分裂过程中染色体的行为等.发现锯缘青蟹染色体数目为2n=98,n=49;染色体核型为2N=40M 22SM 36T.NF=168;结果表明银染技术能揭示中期染色体的遗传特征等内容.本文还对经改进的染色体研究技术在锯缘青蟹染色体研究中的应用效果进行了分析和讨论。  相似文献   

17.
Concerns about the environmental impacts of mariculture have grown in recent years in response to the rapid expansion of the industry. The blue mussel (Mytilus edulis) is the main product of shellfish mariculture in the Northeast Atlantic and Baltic Sea, with approximately one third of the harvest cultured using suspended longlines within sheltered marine areas. The main aim of this study was to examine the interactions, and assess the impacts (if any) of mussel suspension culture on the seabird and seal community, employing a simultaneous study of culture and control sites. The study spanned a 20-month period (from November 2001 to August 2003) and encompassed six sites in Bantry Bay (Southwest Ireland).There was no significant difference in species richness between mussel and control sites. Similarly, species diversity did not significantly differ between the mussel and control sites although control sites were generally more diverse than mussel sites, the latter particularly dominated by large numbers of Laridae.Significantly higher numbers of Phalacrocoracidae, Laridae and Alcidae were recorded in mussel sites than in control sites. However, no significant difference was found between Gaviidae or common seal (Phoca vitulina) numbers in mussel and control sites. Seasonal patterns of abundance were similar in mussel and control sites, with peak numbers of most species groups occurring in spring.Mussel suspension culture does not appear to have an adverse effect on the abundance of seabirds or common seals in this area. The safe perching platforms provided by suspension culture floats, combined with a number of other factors, contribute to an increased abundance of a number of seabird species, particularly Laridae. The possible interactions between vertebrate predators and mussel suspension aquaculture are discussed and possible explanations for the increased seabird abundance observed in these areas are offered.  相似文献   

18.
近距离照射联合贻贝提取物对荷瘤鼠治疗效果的研究   总被引:1,自引:0,他引:1  
观察了近距离照射联合贻贝提取物对U14鼠免疫功能的影响,以期探讨贻贝提取物在放射治疗中的作用。结果表明,贻贝提取物使荷瘤鼠因近距离照射而下降的免疫指标明显回升,包括脾脏NK细胞杀伤活性,丝裂原诱发的淋巴细胞转化强度和外周血白细胞总数及脾指数,亦有效增强放疗的治疗效果。  相似文献   

19.
The paper preliminarily discusses the effects of cadmium pollution on the alkaline phosphatase of mussel, mytilue viridid. The results show the cadmium pollution has a inhibition on the alkaline phosphatase, and the degree of inhibition rises with the increases of concerntration if cadmium in seawater. When the concentration is within the range of 1-500 ppb, the percentage of inhibited emzyme has a linear relationship with the logarithm of cadmium concentration. The results also prove that the salinity may increase the effects of cadmium on emzyme. The inhibition which may be indirect or secondary effects is reversible.Mussels play a purification role in the waste water containing cadmium, the purification rate of which may as high as 125 mg Cd2 gram (mussel)/day. This is a prospective method in purification and removing of cadmium.The study of effects of cadmium pollution on the alkaline phosphatase of mussel shows that in the study of effects of pollutants on aquatic organisms, biochemical index may  相似文献   

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