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1.
岩藻聚糖硫酸酯体外抗氧化特性的研究 总被引：2，自引：2，他引：0
采用化学发光分析法研究岩藻聚糖硫酸酯清除超氧阴离子自由基 (O· -2 )及羟基自由基(· OH)的作用 ,并以高效液相色谱法研究对卵磷脂 (PC)过氧化的抑制能力。结果显示 :(1)三种岩藻聚糖硫酸酯组分均有清除活性氧自由基的能力 ,但清除超氧阴离子自由基的能力高于清除羟基自由基的能力。其中糖醛酸含量高和硫酸酯含量低的组分 清除自由基的能力最强 ,它对 O· -2 的IC50 为 0 .0 4 4 mg/m L,对· OH的 IC50 为 0 .0 6 2 mg/m L。 (2 )岩藻聚糖硫酯具有抑制 PC过氧化的能力。岩藻聚糖硫酸酯组分受活性氧损伤后 ,它们的抗氧化能力均降低。研究表明 ,岩藻聚糖硫酸酯具有较强的体外抗氧化的功能。 相似文献
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为了立体选择性合成全甲基化没食子儿茶素没食子酸酯(GCG),本文以全甲基化表没食子儿茶素没食子酸酯(EGCG)为原料,通过酯水解和Mitsunobu反应立体选择性地合成了构型C-3位翻转的目标产物全甲基化GCG。全甲基化GCG经水解得到5,7,3′,4′,5′-五氧甲基没食子儿茶素(GC),再经Mitsunobu反应得到全甲基化EGCG。经核磁和旋光验证,所合成的全甲基化EGCG与原料全甲基化EGCG相同。核磁测试证实两次Mitsunobu反应产物C-3均发生立体构型翻转。初步细胞实验表明1μmol/L全甲基化GCG与紫杉醇共同作用于P-gp高表达LCC6/MDR乳腺癌耐药细胞株时,紫杉醇IC50为10.1nmol/L,全甲基化GCG逆转倍数(RF)达到13.9。 相似文献
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甲壳质及壳聚糖的硫酸酯化反应是其化学修饰中最吸引人的研究内容之一。由于它们与肝素有相似骨架， 经硫酸酯化，引入-NHSO3H， - CH2OSO3H - OSO3H， COOH，-CH2COOH基团后得到甲壳质(壳聚糖)类肝素药物，显示出抗栓、抗凝血性能(李鹏程等，1998；曾宪放等，1992；Proeyanat et al.，2002)；如Muzzarelli(1985)早已预见并证实其抗凝血活性；莫斯科血液研究中心病理药理系止血组的研究人员也证明了壳聚糖硫酸酯具有抗凝血活性，他们将肝素与壳聚糖硫酸酯以1∶1的比例给家兔静脉注射各0.5mg/kg，与单纯用肝素1mg/kg对照组比较，其抗凝血效应基本接近，但对照组有出血副作用，而试验组无副作用，可以证明壳聚糖硫酸酯具有既抗凝血又止血的双重调节作用；Tokura等(1994)证实分子质量大小和脱乙酰度不同对磺化产物的抗凝血活力有影响，样品分子质量26000 Da的活性最高，脱乙酰度70%比45%的抗凝血活性更强，脱乙酰度为95%的壳聚糖磺化后抗凝血活性最弱。在中国这方面的研究相对较少，特别是分子中含硫的多寡对生物活性的影响还未见报道。本文作者就壳聚糖的磺化产物对大鼠抗栓、抗凝血进行了研究，表明壳聚糖硫酸酯具有一定的抗栓、抗凝血效果，且具有一定的量效关系，这对今后该方面的进一步研究提供了科学理论依据。 相似文献
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四种齐墩果酸皂苷的合成 总被引：1，自引：0，他引：1
为获得高活性齐墩果酸皂苷药物候选分子，报道了4种单糖(葡萄糖、半乳糖、木糖、阿拉伯糖)与齐墩果酸的D3羟基进行糖苷化反应合成皂苷的方法。在合成中采用糖的全苯甲酰化三氯乙酰亚胺酯为糖基供体，齐墩果酸烯丙醇酯为糖基受体，在三氟甲磺酸三甲基硅醇酯(TMSOTf)的作用下，糖基供体与齐墩果酸的C-3羟基进行糖苷化，以较高的收率和专一的立体选择性得到4种齐墩果酸单糖皂苷。化合物结构经核磁共振波谱得以确证。 相似文献
5.
褐藻多糖硫酸酯是一种含有硫酸基的水溶性杂聚糖,其化学组成对抗氧化活性有较大的影响。本文对三种褐藻来源的褐藻多糖硫酸酯及其降解产物化学组成和抗氧化活性进行了研究,阐明了不同来源褐藻多糖硫酸酯体外抗氧化活性的构效关系。实验结果表明:1)分子量对抗氧化活性有较大影响,但是对不同褐藻来源的褐藻多糖硫酸酯的影响趋势并不一致。2)岩藻糖、硫酸基和糖醛酸含量对清除超氧阴离子自由基的影响与分子量有一定的关系。对于低分子量样品,岩藻糖和硫酸基含量与抗氧化活性成正相关。3)岩藻糖和硫酸根的比值对羟基自由基的清除能力有一定影响,比值越大,羟基自由基的清除能力越强。分子量、岩藻糖、硫酸基和糖醛酸的含量对褐藻多糖硫酸酯的抗氧化活性的影响依次减小。本研究为褐藻多糖硫酸酯在抗氧化剂保健品和功能食品中的应用提供了基础数据,也为裙带菜、羊栖菜和铜藻的高值化利用提供了理论依据。 相似文献
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灰树花糖蛋白中总糖含量的测定 总被引：7，自引：1，他引：7
探讨糖蛋白中总糖含量测定的简捷方法,以2种灰树花糖蛋白(GF6A,GF6B)为原料,分别以硫酸-苯酚法和次亚碘酸钠法对其中的总糖含量进行了比较分析。结果显示,应用前者方法测定多糖含量分别为85.45%和38.46%,采用后者方法测定结果分别为87.03%,36.51%,2种方法测定的结果相近,其中以硫酸-苯酚法因不需对样品进行预水解,方法简便、可靠,更适合于糖蛋白中总糖含量测定。 相似文献
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以喜树碱为母体,分别以甘氨酸和己二酸为连接臂与喜树碱的20-羟基以酯键缩合,再将其分别与半乳糖、甘露糖、乙酰氨基葡萄糖、乳糖的衍生物缩合定向半合成了3个水溶性(4-6)和1个脂溶性的喜树碱-糖缀合物(7),通过核磁共振氢谱、碳谱和高分辨质谱对化合物结构进行了确证。对已有的α构型喜树碱糖缀合物(1-3)和本文合成的β构型的喜树碱糖缀合物(4-7)的构效关系进行了初步的探讨,体外抗肿瘤实验表明,水溶性糖缀合物(1-6)具有与喜树碱相当的活性,脂溶性糖缀合物(7)的活性有明显下降。 相似文献
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以海带岩藻聚糖硫酸酯粗多糖为原料,采用Cu2+-H2O2自由基氧化体系结合超滤技术制备低分子量岩藻聚糖硫酸酯.通过比较粗糖质量和H2O2浓度对产物的分子量和化学组成的影响,确定制备高硫酸根高岩藻糖的低分子量岩藻聚糖硫酸酯的最佳条件是:4.5％ H2O2与0.026 7 mol/L Cu2+降解4.5g岩藻聚糖硫酸酯,得到Fa3(Mw7.68kDa)和Fb3(Mw 3.89kDa)2个组分,其硫酸根含量较高,为30.32％和32.48％.Fa3组分主要由岩藻糖(64.25％)和半乳糖(30.74％)组成,而Fb3的硫酸根和半乳糖含量高于Fa3,但岩藻糖含量下降.比较发现,H2O2浓度由4.5％上升为9％时,制备的低分子量岩藻聚糖硫酸酯的分子量、岩藻糖含量和硫酸根含量都下降.研究发现Fa3和Fb3对流感病毒神经氨酸酶具有较强的抑制作用,尤其是Fa3组分活性较高.说明分子量对岩藻聚糖硫酸酯的神经氨酸酶抑制活性影响要大于硫酸根含量,而且Fa3组分对流感病毒神经氨酸酶的抑制活性也可能与其单糖组成较均一有关. 相似文献
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本实验采用群体感应抑制(quorum sensing inhibitory, QSI)活性导向法, 对分离自山东威海近海底泥的真菌WH7-2开展代谢产物研究。综合菌落形态和转录间隔区 (Internal Transcribed Spacer, ITS)全序列分析, 菌株WH7-2鉴定为腐皮镰刀菌Fusarium solani。综合运用多种色谱方法, 从该真菌大米发酵产物的活性部位中分离得到11个化合物。分别鉴定为 (2E, 5E)-3, 5, 7-三甲基-2, 5-辛二烯酸 (1)、不饱和脂肪酸酯混合物 (2—4)、镰红菌素-3-甲醚 (5)、脱水镰红菌素 (6)、(22E)-5α, 8α-过氧化麦角甾-6, 22-二烯-3β-醇(7)、(22E)-5α, 8α-过氧化麦角甾-6, 9 (11), 22-三烯-3β-醇(8)、3β-羟基-胆甾-5-烯-7-酮 (9)、6β-羟基-胆甾-4-烯-3-酮 (10)和(22E)-胆甾-5, 22-二烯-3β-醇 (11)。其中不饱和脂肪酸酯混合物(2—4)具有QSI活性, 其他化合物无QSI活性。除5和6外, 其他化合物均为首次从腐皮镰刀菌F. solani中发现。 相似文献
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港口内靠码头系泊船运动的计算 总被引：11，自引：1，他引：11
本文以英国南海岸处Ｓｈｏｒｅｈａｍ港内系泊船为例，研究了港口内靠码头系泊船运动的数值计算问题。该船的实船实验和模型实验已经完成，为验证本文计算结果提供了依据。运动方程在时域内求解。在计算船体脉冲响应函数时，引入了船体阻尼系数在频率很低时的渐近表达式和一迭代算法。分析了港内共振波浪和其对船体的作用力。讨论了靠码头系泊船运动的非线性特征。计算结果与实验结果符合很好。 相似文献
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An elliptic jet and a square jet flowing into a counterflow with different jet-to-current velocity ratios are investigated by using realizable k–ε model. Some computed mean velocity and scalar features agree reasonably well with experimental measurements, and more features are obtained by analyzing the computed results. After fluid issues from a nozzle, it entrains ambient fluid, and its velocity and concentration on the centerline decay with the distance downstream from the potential core (l0). The decay ratio increases with the decreasing jet-to-current velocity ratio α. For an elliptic jet, the evolution of the excess velocity half-width b and the concentration half-width bc merely remains constant near the jet exit on major-axis plane while they increase linearly on the minor-axis plane. However, the half-widths on the major-axis and minor-axis plane become proportional to the axial distance downstream after equaling each other. For a square jet, b and bc increase linearly with the distance downstream from the jet exit, but the spread ratio is larger on the middle plane than that on the diagonal plane before they equal each other. The radial extent of the dividing streamline rs or the mixing boundary rsc increases linearly downstream, and decreases exponentially after reaching a peak at xb. The ratio on the minor-axis plane is larger than that on the major-axis plane for an elliptic jet. The characteristics are the same for the square jet. b, bc, rs, and rsc on two corresponding planes become equal to each other more rapidly for the square jet than for the elliptic jet, because the sharp corner of the square nozzle induces secondary structures that are more intense. The distributions of the excess axial velocity and scalar concentration exhibit self-similarity for either the elliptic jet or square jet in the region of l0 < x < xb. On the cross section, four counter-rotating pairs of vortices, which enhance the entrainment between the jet and counterflow, form at the four corners of the square jet or at the two ends of the major-axis plane of the elliptic jet. The recirculation pattern formed by these axial vortices is more complex for the square jet than that for the elliptic jet. The turbulent kinetic energy k have large value in the region near the jet exit and stagnation point. The maximum value of k for the square jet is larger than that of the elliptic jet near the jet exit. This results in the square jet mixing more strongly than the elliptic jet. 相似文献
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提出一种基于序贯预测误差方法（ＳＰＥ）的多层神经网络（ＭＮＮ）的学习算法。经模拟计算，它比传统的基于最陡下降方法的误差反传（ＳＤＢＥＰ）算法具有更好的收敛性能。并对这两种算法进行了模拟计算的比较． 相似文献
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This paper deals with the study of moisture budget of the typhoon No. 7507. The results show that the mass convergence produced by divergent wind has made the maximum contribution to precipitation, especially at the lower level, accounting for about 70% of the total precipitation, and that the advection term of water vapor is very small. The vertical flux term is to transport water vapor from the lower level to the middle and upper levels, resulting in the increase of water vapor accumulation at these levels. Evaporation term also plays an important role in precipitation, accounting for about 13% of the total precipitation and 23% of the horizontal moisture convergence. The moisture of the typhoon comes mainly from its southern boundary and the secondary inflow of moisture is from its western or eastern boundary. All the inflow layers of these three boundaries are very deep, reaching up to 300 hPa, while the strongest inflow occurs below 700 hPa. In the northern boundary of the typhoon, we obtained that 相似文献
16.
A Time-Domain Coupled Model for Nonlinear Wave Forces on A Fixed Box-Shaped Ship in A Harbor 总被引：2，自引：1，他引：1
A 2-D time-domain numerical coupled model is developed to obtain an efficient method for nonlinear wave forces on a fixed box-shaped ship in a harbor.The domain is divided into an inner domain and an outer domain.The inner domain is the area beneath the ship and the flow is described by the simplified Euler equations.The other area is the outer domain and the flow is defined by the higher-order Boussinesq equations in order to consider the nonlinearity of the wave motions.Along the interface boundaries between the inner domain and the outer domain,the volume flux is assumed to be continuous and the wave pressures are equal.Relevant physical experiment is conducted to validate the present model.It is shown that the numerical results agree with the experimental data.Compared with the coupled model with the flow in the inner domain governed by the Laplace equation,the present coupled model is more efficient and its solution procedure is more simple,which is particularly useful for the study on the effect of the nonlinear wave forces on a fixed box-shaped ship in a large harbor. 相似文献
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中国隆头鱼之一新属新种——尖尾新丝隆头鱼 总被引：1，自引：0，他引：1
在过去几年的西沙群岛鱼类区系调查中,我们曾在西沙群岛的东岛和永兴岛附近水域,先后三次采到5尾前所未曾见过的隆头鱼类标本,经过比较研究后,认为是一新属、新种,定名为“尖尾新丝隆头鱼”,(Neocirrhilabrus oxyurus gen. et sp. nov.)现描述如下: 相似文献
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王荣良 《中国海洋大学学报(自然科学版)》1988,(1)
本文对J.Maynard Smith提出的物种竞争模式: x=x(a-bx-cy-kxy) y=y(e-fx-gy-lxy)作了定性分析,并给出两物种共存或其中任一物种死亡,而另一物种趋于确定值的条件。 相似文献
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An edge wave is a kind of surface gravity wave basically travelling along a shoaling beach. Based on the periodic assumption in the longshore direction, a second order ordinary differential equation is obtained for numerical simulation of the cross-shore surface elevation. Given parameters at the shoreline, a cross-shore elevation profile is obtained through integration with fourth-order Runge-Kutta technique. For a compound slope, a longshore wavenumber is obtained by following a geometrical approach and solving a transcendental equation with an asymptotic method. Numerical results on uniform and compound sloping beaches with different wave periods, slope angles, modes and turning point positions are presented. Some special scenarios, which cannot be predicted by analytical models are also discussed. 相似文献
20.
This is a numerical study on the time development of surface waves generated by a submerged body moving steadily in a two-layer fluid system, in which a layer of water is underlain by a layer of viscous mud. The fully nonlinear Navier-Stokes equations are solved on FLUENT with the Volume-of-Fluid (VOF) multiphase scheme in order to simulate the free surface waves as well as the water-mud interface waves as functions of time. The numerical model is validated by mimicking a reported experiment in a one-layer ... 相似文献