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1.
采用Mini-Beadbeater组织研磨器破碎的方法进行了提取霞水母刺丝囊细胞毒素的研究。首先制备霞水母刺丝囊细胞,然后研究破碎频率、破碎时间对提取霞水母刺丝囊细胞毒素浓度的影响。结果表明,组织研磨器能够在2—3min内破碎约90%的霞水母刺丝囊细胞,从而提高水母毒素的提取效率。SDS-PAGE结果显示,提取的水母毒...  相似文献   

2.
通过2009-2011年5月下旬-7月下旬辽东湾北部近海10m等深线内的大型水母调查数据,分析了辽东湾北部近海近三年中大型水母资源状况,并探讨了辽东湾大型水母的生态类型。结果显示:辽东湾北部近海大型水母种类主要有海蜇(Rhopilema esculentum)、沙蜇(Nemopilema nomurai)、白色霞水母(Cyanea nozakii)、海月水母(Aurelia sp.1),海蜇和沙蜇是优势种。海蜇幼水母阶段主要集中分布在5m等深线以内的近岸河口水域,随着个体增大有略向深水或密度较小的水域扩散的趋势,仍主要分布在5m等深线两侧水域,属于高温低盐种类。6月份调查海区中发现大量的沙蜇幼水母,随着沙蜇个体增大,7月份调查海区中沙蜇数量大幅度减少。辽东湾海月水母在南部海域出现较多,2010、2011年在北部近海部分海域出现。白色霞水母近几年来辽东湾出现较少,栖息在盐度较高的水域。辽东湾各种大型水母中,沙蜇的生长速度最快。辽东湾海蜇幼水母、沙蜇幼水母的海区出现时间要晚于黄、东海。  相似文献   

3.
为查清沙蜇大量暴发的原因,采用黄海北部的沙蜇亲体进行自然受精获得了数百万的螅状体,除海月水母和发形霞水母在消化腔和雌体的性腺中完成受精外,上述其余种类均为体外受精,霞水母属的2个种类的浮浪幼虫在形成螅状体前先形成一个具角质的浮浪体囊,沙蜇、海蜇、黄斑海蜇和Stomolophus meleagris螅状体繁殖新螅状体的唯一无性繁殖方式是足囊繁殖,霞水母可通过足囊和由匍匐茎形成囊胞两种方式繁殖新螅状体,海月水母(A.aurita)螅状体繁殖新螅状体的的无性繁殖方式包括足囊繁殖、出芽生殖、匍匐茎生殖、纵向分裂、内繁殖体和外繁殖体生殖,同时有直接发育现象,Rhizostoma pulmo的无性繁殖方式有足囊,出芽生殖,匍匐茎,浮浪体芽。  相似文献   

4.
白色霞水母蛋白抗氧化活性的初步研究   总被引:3,自引:1,他引:2       下载免费PDF全文
通过羟自由基清除作用、超氧自由基清除作用、还原能力和金属螯合能力等抗氧化活性实验,测定了白色霞水母蛋白的抗氧化活性。实验结果表明:白色霞水母蛋白具有较高的清除羟自由基的能力,蛋白质量浓度为41μg/mL时,清除率可达到85%;白色霞水母蛋白对超氧阴离子的清除作用较弱,蛋白质量浓度为77.7μg/mL时,清除率〈50%;白色霞水母蛋白有较强的还原能力,但没有螯合能力;白色霞水母蛋白的抗氧化活性受温度影响不大,且巯基含量不是影响抗氧化活性的主要因素。  相似文献   

5.
2011 年夏季胶州湾三种大型水母的种群动态研究   总被引:4,自引:3,他引:1  
2011年8月1日-9月30日,对胶州湾三种大型水母沙海蛰(Nemopilema nomurai)、白色霞水母(Cyanea nozakii)、海月水母(Aurelia aurita)的种群数量变动及空间分布情况开展了目测调查,同步获得了气象、水温、盐度、叶绿素a、浮游动物丰度、种类组成等数据,调查频率为每周2次。调查期间,海月水母表现出逐渐降低的趋势,沙海蛰与白色霞水母种群均于8月11日达到数量高峰,8月底及9月中旬之后依次衰落,至9月底,三种水母基本消失。三种水母的种群平均丰度变化范围分别为:沙海蛰0-230.8ind/km2;霞水母0-150.2ind/km2;海月水母0-123.4ind/km2。从分布区域来看,海月水母主要位于近岸区,沙海蜇主要分布于湾口和中部深水区,而白色霞水母则在高峰期和次高峰期分别集中于深水区和近岸区。结合往年资料,作者认为,海月水母种群能够在胶州湾内进行自我补充并完成其生活史,而胶州湾沙海蛰与霞水母的种群补充则可能主要依赖于湾外种群。另外,目测方法的准确性可能受到气象条件的影响。  相似文献   

6.
为了探究不同种类水母对浮游食物网的摄食影响,采用碳氮稳定同位素和脂肪酸标记法,研究分析了渤海红沿河海域常见的4种小型水母(伞径直径5 cm:卡玛拉水母Malagazzia carolinae、球形侧腕水母Pleurobrachia globosa、帽铃水母Tiaricodon coeruleus、半球美螅水母Clytia hemisphaerica和3种大型水母(伞径10—200cm:沙海蜇Nemopilema nomurai、海月水母Aurelia aurita)和白色霞水母Cyanea nozakii)的食物组成。结果表明,不同种类水母的食物组成存在差异。其中卡玛拉水母、球形侧腕水母、帽铃水母、半球美螅水母和白色霞水母均偏肉食食性,食物组成中动物性食物所占比例更高,高于海月水母与沙海蜇。在食物粒径谱上,帽铃水母和半球美螅水母较大粒径的食物比例均高于小粒径的悬浮有机物(POM)比例。卡玛拉水母和球形侧腕水母各个粒径食物比例接近。大型水母中,白色霞水母的食物中大粒径的浮游动物的比例高于海月水母,更高于沙海蜇。由此看来,小型水母和大型的白色霞水母的暴发会直接影响大中型浮游动物数量,海月水母的暴发对不同大小的浮游生物均会产生一定的影响。而沙海蜇的暴发会大量摄食1 mm的小型浮游生物和POM,对大中型浮游动物以及更高营养层生物(鱼类等)的影响可能是通过蜇伤以及饵料竞争导致的。因此,不同种类水母暴发对浮游生物的影响存在差异。本研究从摄食角度初步探究了水母对海洋生态系统物质循环和能量流动的影响,为水母暴发的灾害防治提供了数据支持。  相似文献   

7.
从霞水母Cyanea sp.中分离刺丝囊,提取刺丝囊毒素(NV),并研究了温度、pH和几种化学组分对其溶血活性稳定性的影响.实验结果表明,在4~37℃范围内NV的溶血活性与温度变化密切相关,毒素在4℃以下几乎没有发生溶血反应,在37℃以下反应45 min以后,溶血活性达到最大值;毒素的溶血活性对pH敏感,在pH 7.8时.溶血活性最强,溶血率为93.53%.EDTA、NaCl和还原型谷胱甘肽(GSH)对NV溶血活性都具有稳定作用.通过正交实验发现,GSH对溶血活性的影响最大,当提取液中含有1.0 mmol/L GSH和5.0 mmol/L NaCl时毒素的溶血活性最强.DTT(二硫苏糖醇)明显降低毒素的溶血活性,说明毒素中溶血蛋白的活性很可能与分布在蛋白质表面的二硫键有关.Ca2+虽然能够增强毒素的溶血活性但是对毒素并没有稳定作用,所以Ca2+可能只是毒素溶血活性的活化剂.甘油对毒素的稳定作用不明显.  相似文献   

8.
左涛  王俊  吴强  袁伟  栾青杉 《海洋与湖沼》2016,47(1):195-204
2015年5月搭载“北斗”渔业调查船、使用渔拖网的采样方式,在整个黄海及东海北部进行系统的走航式大面调查,记录了30°N—39°N海区内的大型水母种类组成、伞径大小及生物量分布,估算和比较了大型水母与其他渔业生物的生物量。结果表明,5月整个调查区,大型水母的总生物量估算值5.9万t,绝大部分由黄海中部的多管水母和洋须水母生物量贡献所致。出现的大型水母种类伞径分布呈单峰型。不同种类的水母分布具有明显地理区域和水文偏好性。其中,洋须水母主要分布于黄海中、北部50m水深以深水域,多管水母主要分布于黄海中部50m水深以浅的西侧以及整个东海北部;沙海蜇多为幼体,分布于黄、东海交汇区31°N—33°N间;霞水母较为集中出现于31°N以南、123°N以西近海。各水母种类的高密区的底层水温按洋须水母、沙海蜇、四叶小舌水母、霞水母呈升高趋势。东海多管水母分布区的底层水温与沙海蜇相近;黄海多管水母分布区的底层水温较洋须水母略高。沙海蜇和四叶小舌水母较其他水母的适温范围宽。霞水母和洋须水母处于相对高盐区域。  相似文献   

9.
李洁  徐奎栋 《海洋与湖沼》2016,47(3):612-625
大型水母沙海蜇旺发已成为黄海夏季的一个常见的生态现象。然而,沙海蜇旺发与微小型浮游动物尤其是纤毛虫的相互作用关系依然不明。本研究基于2013年6月(水母旺发初期)、8月和9月(水母旺发期)三个航次的调查,对黄海水母高发的I(33°N)、G(34°N)、E(35°N)断面的表层微小型浮游动物的丰度及生物量分布变化与水母发生关系进行探讨。微型鞭毛虫丰度6月平均丰度和生物量分别为357ind./m L和3.92μg C/L,至8月降为145ind./m L和2.55μg C/L,9月回升至160ind./m L和2.84μg C/L。共鉴定纤毛虫31属64种,三个月份纤毛虫的种类组成差别不大。纤毛虫6月的平均丰度和生物量分别为2282ind./L和8.93μg C/L,至8月降为933ind./L和3.92μg C/L,9月回升至2319ind./L和6.55μg C/L。与丰度和生物量的较大变化不同,纤毛虫6月的平均生产力为12.74μg C/(L·d),8月降至7.39μg C/(L·d),9月略增至7.79μg C/(L·d)。桡足类无节幼体仅见于6月(10.59ind./L)和8月(8ind./L),在沙海蜇旺发的8月其平均生物量(0.08μg C/L)和生产力(0.032μg C/(L·d))远低于纤毛虫。本研究表明,在南黄海水母发生过程中,纤毛虫在微小型浮游动物的生物量和生产力中占据了主导地位,并受到水母旺发的直接和间接(级联效应)影响。  相似文献   

10.
孙明  董婧  王爱勇 《海洋与湖沼》2012,43(3):562-567
采用控制光照强度的方法,研究了不同光照强度对白色霞水母无性繁殖的影响。结果表明,400lx组螅状体的相对增长率、特定生长率和螅状体足囊、囊胞繁殖率均显著高于其它各试验组(P<0.05),呈现出400lx>800lx>1200lx>0lx的趋势。在设定的光照强度范围内,螅状体足囊、囊胞繁殖出现的时间随光照强度的减少而延迟。800lx组的足囊萌发率最高,但各试验组足囊萌发率之间无显著性差异(P>0.05)。试验期间始终是400lx组螅状体的横裂率最高,试验结束时,0lx组螅状体的横裂率最低。试验结果说明,适当的光照度对白色霞水母螅状体生长、螅状体足囊、囊胞繁殖和横裂生殖均有促进作用,强光条件或完全无光照条件不适宜白色霞水母螅状体的生长并可使横裂生殖延迟或受到抑制。  相似文献   

11.
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.  相似文献   

12.
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.  相似文献   

13.
The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.  相似文献   

14.
Coastal inundation associated with extreme sea levels is the main factor which leads to the loss of life and property whenever a severe tropical cyclonic storm hits the Indian coasts. The Andhra and Orissa coasts are most vulnerable for coastal inundation due to extreme rise in sea levels associated with tropical cyclones. Loss of life may be minimized if extreme sea levels and associated coastal flooding is predicted well in advance. Keeping this in view, location specific coastal inundation models are developed and applied for the Andhra and Orissa coasts of India. Several numerical experiments are carried out using the data of past severe cyclones that struck these regions. The simulated inland inundation distances are found to be in general agreement with the reported flooding.  相似文献   

15.
16.
《海洋学报(英文版)》2014,(9):F0003-F0003
<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology,  相似文献   

17.
Grain size and water content in box-core sediments from the Clarion-Clipperton fracture zone (C-C zone) in the northeast equatorial Pacific were analyzed in detail to understand the downcore variations across a hiatus between Quaternary and Tertiary layers. Grain-size distributions in the topmost core sediments show two modes: a coarse mode (peaked at 50 μm) and a fine mode (at 2-25 μm). The coarse mode disappears gradually with depth accompanied by the dissolution of siliceous fossil tests, whereas the fine mode coarsens due to the formation of authigenic minerals. Water content increases abruptly across a color boundary between an upper pale brown layer and a lower dark brown layer that is the hiatus between Quaternary and Tertiary layers. Abundant smectites and microvoid molds, which are created by the prolonged fossil dissolution in the underlying sediment, are attributed for the abrupt downcore variation of water content. Overall variations in grain size and water content in the topmost core sediments in the western C-C zone are possibly constrained by the dissolution of biogenic siliceous fossils. Variations in geotechnical properties related to these changes must be considered in the design of nodule collectors.  相似文献   

18.
This article concerns an interrelation between the sea levels and the western boundary flow near a tectonic boundary in a local zone in the Northwestern Pacific. In this zone, sea level variations at stations located on the coast facing the Pacific are studied to find the interrelation between variations of the Kurosio flow as an index of the distance of the flow axis off a specific coast. The result is discussed after data processing of the monthly means of the sea levels, and a notice is taken of variations caused by active crustal upheavals during a seismic event, a local earthquake.  相似文献   

19.
This study tests the hypothesis that the in situ void ratio of surficial marine sediments may be predicted from shear wave velocity-depth data with a reliability equal to that of other methods currently available. Shear wave velocity is fundamentally controlled by the number of grain-to-grain contacts per unit volume of material and by the effective stress across those contacts. In this study, three previously established empirical formulae are used to predict void ratio from velocity-depth data. Field data were acquired along a transect off the northern Californian coast across which water depth increased from 35 to 70 m and seafloor sediment type varied from sand to silty-sand, respectively. A towed seafloor sled device was used to collect shear wave refraction data, and a marked, systematic decrease in velocity was observed along the line, ranging from 35-70 m/s for the coarse, near-shore material to 25-40 m/s for the finer, offshore deposits. Void ratios predicted from these velocities were compared with data measured directly from box-core samples. Of the formulae used for prediction, two agree remarkably well with the control data. Both predicted and control values increase from 0.6-0.8 for the sandy material to 1.1-1.5 for the silty-sand. Thus, this study does not disprove the hypothesis set and demonstrates the potential of field shear wave velocity-depth data as a means of delineating spatial variation in void ratio for surficial marine sediments in a remote, nondestructive manner.  相似文献   

20.
Particulate samples were collected from the Changjiang river system during a flood period, in May 1997, and POC, stable isotope and lipids associated with particles were examined. Results showed the decrease (0.84% ~ 1.88%) of organic carbon content from the upper reaches to the estuary.δ13C values of particulate organic carbon was in the range of -24.9×10-3 to -26.6×10-3, which were close to the isotopic signature of continental C3 vegetation. Total particulate n-alkanes concentrations varied from 1.4 to 10.1μg/dm3,or from 23.7 to 107μg/g of total suspended matter. Fatty acids were present in all the samples, from 1.4 to 5.4μg/dm3, with saturated and unsaturated straight-chain and branched compounds in the carbon number range from C12 to C30. Both δ13C and the ratio of carbon content to nitrogen content indicate the predominance of terrestrial inputs (soil organic matter) among the particles. The biomarker approach has been used to identify the relative portion of terrigenous and autochthonous fraction in the particulate samples. The distribution of fatty acids suggests a striking phytoplanktonic and microbial signal in most particle samples. The terrestrial alkanes are used to estimate the contribution of terrestrial inputs along the mainstream.  相似文献   

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